Lc qtof

Manufactured by Agilent Technologies

Sourced in United States

The Agilent LC-QTOF is a high-performance liquid chromatography-quadrupole time-of-flight mass spectrometer. It is designed to provide accurate mass measurements and high-resolution mass analysis for a wide range of applications.

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Lab products found in correlation

Zorbax rrhd eclipse plus c18 column, by Agilent Technologies (1 mentions) 8453 spectrophotometer, by PerkinElmer (1 mentions) Dip 700 polarimeter, by Jasco (1 mentions) 1600 series ftir, by PerkinElmer (1 mentions) Lc 20at solvent delivery module, by Shimadzu (1 mentions) Spdm20a vp diode photodiode array detector, by Shimadzu (1 mentions) Scl 20a vp system controller, by Shimadzu (1 mentions) Circular dichroism spectrometer, by Applied Photophysics (1 mentions) Lc tof, by Agilent Technologies (1 mentions)

6 protocols using lc qtof

Quantitative Analysis of Pseudomonas Metabolites

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This experiment was also performed by LC/Q-ToF (Agilent) using ZORBAX RRHD Eclipse Plus C18 column (Agilent) in MS mode, with 1 μL injection of experimental samples and standards for pyochelin, phenazine-1-carboxylic acid, and rhamnolipids. The mobile phase was water (A) and acetonitrile (B), both with 0.1% (v/v) formic acid. The elution procedure was 5% B between 0 and 1 min; 5−95% B between 1 and 11 min; 95% B between 11 and 13 min; 95−5% B between 13 and 14 min; and 95% B between 14 and 16 min with a flow rate of 0.4 mL/min.
The standards were dissolved in methanol to make stock solutions at a concentration of 1 g/L for pyochelin, 0.1 g/L for phenazine-1-carboxylic acid, and 10 g/L for rhamnolipids. The stock solutions were diluted to appropriate concentrations for sample preparation.

Donadt T.B., Wu Y., Lang J., Li S, & Yang R. (2021). Design of Polymeric Thin Films to Program Microbial Biofilm Growth, Virulence and Metabolism. Biomacromolecules, 22(12), 4933-4944.

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Comparative Metabolomics Analysis of Colorectal Cancer

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The data of the same 18 CRC samples and 20 healthy controls, collected in our previous study,^{15 (link)} were used to demonstrate the performance of a traditional large targeted MS assay (with ∼160 metabolites in the detection list). Although not exactly the same, the LC conditions of that assay were very similar to those of the Agilent LC–QQQ or LC–Q-TOF in this study. The experimental methods were detailed in our previous paper.^{15 (link)}

Gu H., Zhang P., Zhu J, & Raftery D. (2015). Globally Optimized Targeted Mass Spectrometry: Reliable Metabolomics Analysis with Broad Coverage. Analytical chemistry, 87(24), 12355-12362.

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LC-MS Analysis of Metabolite Profiles

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LC–MS analyses were performed on a LC-QTOF (Agilent). Water and acetonitrile with 0.1 % formic acid were considered as mobile phase at a flow rate of 300 nL/min. The gradient system increases from 5 % B to 20 % B linearly for 15 min, then maintains at 20 % B for 10 min before increasing to 100 % B in 1 min and maintaining at 8 min before the next sample [35 ]. The injection sample volume was 10 μL with a run time of 32 min. To reduce any carry-over, two blank samples (3 μL of mobile phases A and B at a 95:5 ratio) were run between the experimental sample injections. The MS system was operated under positive electro-spray ionization (ESI). The chromatographic peaks, signal reproducibility and analyze stability were all checked by analyzing pooled QC samples at regular intervals throughout the batch.

Jothi R., Kamaladevi A., Muthuramalingam P., Malligarjunan N., Karutha Pandian S, & Gowrishankar S. (2024). Untargeted metabolomics uncovers prime pathways linked to antibacterial action of citral against bacterial vaginosis-causing Gardnerella vaginalis: An in vitro and in vivo study. Heliyon, 10(6), e27983.

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Spectroscopic Characterization of Organic Compounds

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Optical rotations were measured on a Jasco-DIP-700 polarimeter at the sodium line (589 nm). UV spectra were obtained on a Hewlett-Packard 8453 spectrophotometer and IR spectra were measured as a thin film on a CaF₂ disc using a Perkin Elmer 1600 series FTIR. ECD measurements were obtained on a Chirascan Circular Dichroism Spectrometer with the samples dissolved in MeOH and placed in a 1 cm quartz cuvette with a solvent subtraction for baseline correction. NMR spectra were acquired on a Varian Inova Unity 500 MHz spectrometer operating at 500 (¹H) or 125 (¹³C) MHz using the residual solvent signals as an internal reference. Samples were in 3 mm Shigemi tubes during NMR analyses. High-resolution mass spectrometry data were obtained on an Agilent LC-TOF or LC-QTOF with ES ionization. Gradient separations used a Shimadzu system consisting of LC-20AT Solvent Delivery Modules, an SPD-M20A VP Diode Photodiode Array Detector, and a SCL-20A VP System Controller. TLC analyses were performed on Si₆₀F₂₅₄ plates and visualized under UV or by heating after spraying with a 1% anisaldehyde solution in acetic acid:H₂SO₄ (50:1). Samples were weighted on a Mettler Toledo analytical balance.

Dai J., Philbin C.S., Wakano C., Yoshida W.Y, & Williams P.G. (2023). New Nostocyclophanes from Nostoc linckia. Marine Drugs, 21(2), 101.

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Compound Solubility Determination in Saline

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Compound solubility was determined
in a saline solution by equilibrating an excess of solid compound
(10 mg/mL) at 25 °C for 24 h with shaking. The samples were spun
down, and the supernatant was analyzed on LC-qTOF (Agilent Technologies)
with injection volumes of 1, 5, 10, and 20 μL. Calibration curves
were plotted by dissolving the compounds to a final concentration
of 1 mg/mL in DMSO. The solutions were further diluted to 100 μg/mL,
and different volumes were injected.

Koteva K., Xu M., Wang W., Fiebig-Comyn A.A., Cook M.A., Coombes B.K, & Wright G.D. (2023). Synthetic Biology Facilitates Semisynthetic Development of Type V Glycopeptide Antibiotics Targeting Vancomycin-Resistant Enterococcus. Journal of Medicinal Chemistry, 66(13), 9006-9022.

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Bioactive Compound Characterization by LC-QTOF

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The characterization of bioactive compounds was carried out by LC-QTOF (Agilent Technologies, Santa Clara, CA, USA, Model no: 6545 QTOF).

Sasidharan S., KP S., Bhaumik A., Kanti Das S, & Nair J H. (2022). Administration of Caesalpinia bonduc Seed Extracts Ameliorates Testosterone-Induced Benign Prostatic Hyperplasia (BPH) in Male Wistar Rats. Research and Reports in Urology, 14, 225-239.

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