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Anti rab37

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Anti-Rab37 is a primary antibody that targets the Rab37 protein. Rab37 is a member of the Rab family of small GTPases, which are involved in the regulation of vesicular transport and membrane trafficking within cells. The Anti-Rab37 antibody can be used in various laboratory techniques, such as Western blotting, immunoprecipitation, and immunohistochemistry, to detect and study the Rab37 protein.

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Lab products found in correlation

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2 protocols using anti rab37

1

Protein Isolation and Western Blot Analysis

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Total protein was isolated from lung tissues and RAW264.7 cells using RIPA lysis buffer (Beyotime, Shanghai, China). The protein concentration was measured using a BCA kit (KeyGen Biotech). An equal volume per sample was subjected to 10% SDS-PAGE and the proteins were transferred onto PVDF membranes (Millipore, Bedford, MA). The membranes were blocked in 5% non-fat milk and probed with anti-GGPPS1 (1:1000, Santa Cruz Biotechnology, Santa Cruz, CA), anti-IL-1β (1:1000, Abcam), anti-IL-6 (1:1000, Abcam), anti-IL-18 (1:200, Abcam), anti-TNF-α (1:1000, Abcam), anti-LC3B (1:200, Abcam), anti-ATG5 (1:1000, Cell Signaling Technology, Danvers, MA), anti-Beclin1 (1:1000, Cell Signaling Technology), anti-Rab37 (1:500, Abcam), and anti-Actin (1:1000, Santa Cruz Biotechnology). After washing, the membranes were probed with HRP-conjugated secondary antibodies (1:2000, Abcam) and the signal was detected by the classical ECL method.
Wang Z., Chen M., Pan X., Wang L., Yin C., Lin Q., Jiang J., Zhang Y, & Wan B. (2022). Knockout of GGPPS1 restrains rab37-mediated autophagy in response to ventilator-induced lung injury. Human Cell, 35(3), 871-884.
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2

Imaging Flow Cytometry for Immune Cell Analysis

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For imaging flow cytometry experiments: anti-CD8 APC-Cy7 (BD Biosciences, San Jose, CA or Tonbo Biosciences, San Diego, CA) and anti-CD56 PE-CF594 (BD Biosciences) were used for surface staining; all intracellular stains included anti-perforin D48 PE or BV421 (Biolegend, San Diego, CA) and anti-perforin δG9 FITC (Biolegend) and one of the following unconjugated antibodies: anti-CD71 (Cell Signaling Technology, Danvers, MA), anti-granzyme B (BD Biosciences), anti-rab3D (Abcam, Cambridge, MA), anti-rab4 (Pierce Antibodies, Waltham, MA), anti-rab5 (Cell Signaling Technology), anti-rab7 (Santa Cruz Biotechnology, Dallas, TX), anti-rab8 (Cell Signaling Technology), anti-rab11a (Cell Signaling Technology), anti-rab27a (Abcam), anti-rab35 (Abcam), anti-rab37 (Abcam), anti-syntaxin6 (Cell Signaling Technology), anti-syntaxin7 (R&D systems, Minneapolis, MN), anti-vti1b (Abcam), anti-VAMP3 (Abcam), anti-VAMP4 (Abcam), or anti-SNAP23 (Abcam). The unconjugated antibodies were detected using goat anti-rabbit AF647, chicken anti-goat AF647, or donkey anti-sheep AF647 secondary antibodies (Invitrogen, Carlsbad, CA). For confocal microscopy, perforin D48 FITC (Abcam), perforin δG9 AF647 (Biolegend), and goat anti-rabbit or donkey anti-sheep AF568 secondary antibodies (Invitrogen) were used, along with the selected rab or SNARE antibody.
Lesteberg K.E., Orange J.S, & Makedonas G. (2017). Recycling endosomes in human cytotoxic T lymphocytes constitute an auxiliary intracellular trafficking pathway for newly synthesized perforin. Immunologic research, 65(5), 1031-1045.
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