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Pf431396

Manufactured by Selleck Chemicals
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Sourced in United States

PF431396 is a laboratory equipment product. It is designed for use in scientific research and analysis. The core function of this product is to provide a specialized tool for conducting various experiments and tests in a laboratory setting.

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Lab products found in correlation

Pf 573228, by Selleck Chemicals (2 mentions) Ccg 1423, by Selleck Chemicals (1 mentions) Y 27632, by Cell Signaling Technology (1 mentions) Pf 562271, by Selleck Chemicals (1 mentions) Pd98059, by Cell Signaling Technology (1 mentions) Verteporfin, by Merck Group (1 mentions) Sb431542, by Selleck Chemicals (1 mentions) U0126, by Cell Signaling Technology (1 mentions) Vs 6063, by Selleck Chemicals (1 mentions) Recombinant human hgf, by R&D Systems (1 mentions) Dasatinib, by Selleck Chemicals (1 mentions) Nocodazole, by Merck Group (1 mentions) Paclitaxel, by Selleck Chemicals (1 mentions) P fak tyr397, by Cell Signaling Technology (1 mentions) Blebbistatin, by Merck Group (1 mentions) Y 27632 2hcl, by Selleck Chemicals (1 mentions) Tae226, by Selleck Chemicals (1 mentions) Ml141, by Selleck Chemicals (1 mentions) Bosutinib, by Selleck Chemicals (1 mentions) Epothilone b, by Selleck Chemicals (1 mentions)

5 protocols using pf431396

1

Chemical Reagents for Signaling Pathways

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Chemical reagents U0126 (Cell Signaling Technology, #9903), PD98059 (Cell Signaling Technology, #9900), Y-27632 (Cell Signaling Technology, #13624), SB431542 (Selleckchem, S1067), CCG1423 (Selleckchem, S7719), verteporfin (Sigma, SML0534). VS-6064 (Selleckchem, S7654), PF431396 (Selleckchem, S7644), PF562271 (Selleckchem, S2890), and Vanadate (New England Biolabs, P0758) were used at doses and times indicated in figure legends. All compounds were dissolved in DMSO.
Sato T., Verma S., Andrade C.D., Omeara M., Campbell N., Wang J.S., Cetinbas M., Lang A., Ausk B.J., Brooks D.J., Sadreyev R.I., Kronenberg H.M., Lagares D., Uda Y., Pajevic P.D., Bouxsein M.L., Gross T.S, & Wein M.N. (2020). A FAK/HDAC5 signaling axis controls osteocyte mechanotransduction. Nature Communications, 11, 3282.
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2

Recombinant HGF Assay with Inhibitors

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Recombinant human HGF was purchased from R&D Systems (Minneapolis, MN, USA). PF-573228, PF-431396, and VS-6063 were purchased from Selleck Chemicals (Houston, TX, USA) and the stock solutions were prepared in DMSO and stored at -20°C.
Kanteti R., Mirzapoiazova T., Riehm J.J., Dhanasingh I., Mambetsariev B., Wang J., Kulkarni P., Kaushik G., Seshacharyulu P., Ponnusamy M.P., Kindler H.L., Nasser M.W., Batra S.K, & Salgia R. (2018). Focal adhesion kinase a potential therapeutic target for pancreatic cancer and malignant pleural mesothelioma. Cancer Biology & Therapy, 19(4), 316-327.
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3

Regulation of Cytoskeletal Dynamics

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We used the following reagents: IMB5046, Vincristine and Vinblastine from J&K Scientific Ltd.; Colchicine from SERVA Feinbiochemica; SiR‐tubulin, SiR‐actin and Rho Inhibitor I (CT04) (C3 exoenzyme covalently linked to a cell penetrating moiety) from Cytoskeleton Inc; PF‐573228 (PF‐228), PF‐431396, TAE226, Y‐27632 2HCl, Dasatinib, Bosutinib, EHop‐016, SB203580, ML141, Paclitaxel, Epothilone B and cRGD (Arg‐Gly‐Asp) peptide from Selleck Chemicals; Blebbistatin, ML‐7, ML‐9 and Nocodazole from Sigma Aldrich; all chemicals used were of analytical grade. Following antibodies were used: p‐FAK(Tyr397)(D20B1), FAK(D2R2E), p‐MLC(Ser19)(3675), MLC(D18E2), p‐HSP27(Ser82)(D1H2F6), HSP27(D6W5V) and GEF‐H1(55B6) from Cell Signaling Technology; β‐Actin(TA‐09), FITC‐conjugated anti‐Mouse IgG, HRP‐conjugated anti‐Mouse IgG and HRP‐conjugated anti‐Rabbit IgG from Zhongshan Golden Bridge Biotechnology.
Zheng Y., Gong J, & Zhen Y. (2020). Focal adhesion kinase is activated by microtubule‐depolymerizing agents and regulates membrane blebbing in human endothelial cells. Journal of Cellular and Molecular Medicine, 24(13), 7228-7238.
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4

Inhibition of FAK signaling in HCC

Cited 1 time
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SOR, PND1186 and PF431396 (Selleck Chemicals, Houston, TX, USA) and TAE226 (kindly provided by Novartis Pharm) were dissolved in dimethyl sulfoxide and stored at − 80 °C until time of use. HCC cells were exposed to different concentration of drugs for different times as detailed in the results.
Cells were lentivirally silenced for FAK as already described [13 (link)].
Romito I., Porru M., Braghini M.R., Pompili L., Panera N., Crudele A., Gnani D., De Stefanis C., Scarsella M., Pomella S., Levi Mortera S., de Billy E., Conti A.L., Marzano V., Putignani L., Vinciguerra M., Balsano C., Pastore A., Rota R., Tartaglia M., Leonetti C, & Alisi A. (2021). Focal adhesion kinase inhibitor TAE226 combined with Sorafenib slows down hepatocellular carcinoma by multiple epigenetic effects. Journal of Experimental & Clinical Cancer Research : CR, 40, 364.
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5

Detailed Signaling Pathway Profiling

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Anti-phospho-S473-Akt, anti-phospho-T308-Akt, anti-Akt1, anti-total Akt, anti-phospho-4E-BP1, anti-4E-BP1, anti-phospho-S6K1, anti-S6K1, anti-phospho-S6, anti-S6, anti-phospho-FoxO3, anti-FoxO3, anti-phospho-GSK3 α/β, anti-GSK3 α/β, anti-phospho-IGF1R, anti-IGF1R, anti-phospho-IRS, anti-IRS, anti-phospho-ERK1/2, anti-ERK1/2, anti-TSC2, anti-mTOR, anti-Rictor, and anti-phospho-tyrosine antibodies were purchased from Cell Signaling Technology. Anti-phospho-S422-SGK and anti-Grb10 antibodies were obtained from Santa Cruz Biotechnology. Rapamycin, Torin1, Ku-0063794, BKM-120, GDC-0941, AZD8931, Erlotinib, BMS754807, OSI906, PF431396, PF573228, and SB273005 were purchased from Selleckchem. Cpd22 and PDGFR inhibitor III were purchased from Calbiochem. Anti-integrin α2, anti-integrin αV, and anti-integrin α5 antibodies for flow cytometry were obtained from BD Biosciences. BTT3033 and anti-integrin α2 antibodies for immunoblot analysis were obtained from Tocris and SantaCruz Biotechnology, respectively. Anti-actin and anti-vinculin antibodies, N-methyl-2-pyrrolidone, PEG400, and lentiviral shRNA plasmids were purchased from Sigma.
Yoon S.O., Shin S., Karreth F.A., Buel G.R., Jedrychowski M.P., Plas D.R., Dedhar S., Gygi S.P., Roux P.P., Dephoure N, & Blenis J. (2017). Focal adhesion- and IGF1R-dependent survival and migratory pathways mediate tumor resistance to mTORC1/2 inhibition. Molecular cell, 67(3), 512-527.e4.
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