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Pa800 plus pharmaceutical analysis ce system

Manufactured by AB Sciex
Sourced in United States

The PA800 Plus Pharmaceutical Analysis CE system is a capillary electrophoresis (CE) instrument designed for pharmaceutical analysis. It provides high-resolution separation and detection of a wide range of analytes. The system features automated sample handling, temperature control, and data analysis software.

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2 protocols using pa800 plus pharmaceutical analysis ce system

1

CE-SDS Analysis of Various rAAV Serotypes

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CE-SDS analyses of VPs obtained from different rAAV serotypes were performed on a PA800 Plus Pharmaceutical Analysis CE system (SCIEX, Framingham, MA, USA) equipped with a laser-induced fluorescence (LIF) detector (488 nm excitation with 600 nm emission filter). CE-SDS separations were performed at 20 cm separation distance (bare fused silica capillary 50 µm inner diameter, 30 cm total length). All separations were performed at 25 °C. Samples were stored at 10 °C. A reverse polarity (cathode on the injection side) was employed, and voltage of 15 kV was applied. The samples were injected at 5.0 kV for 60 s (with a pre-injection of water at 20.0 psi for 0.4 min). The separating capillary was rinsed with 0.1 M NaOH for 10 min, 0.1 M HCl for 5 min, MilliQ-Water for 2 min, and finally SDS-MW separating matrix for 10 min. Data acquisition and analysis were performed using 32 KaratTM Software 10. These reference conditions were adapted from [23 ].
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2

Quantitative Analysis of rAAV6 Capsids

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The 7 × 1010 vg of the rAAV6 unbound fraction and 5 × 1010 vg of the rAAV6 bound fraction was mixed with 14.4 μL 10% SDS (Nippon Gene, Toyama, Japan) and 4.83 μL 2-mercaptoethanol (Nacalai Tesque), respectively. Each mixture was incubated at 70°C for 3 min, and the buffer was then exchanged twice with 70% matrix exchange solution (0.5 mL 2-mercaptoethanol and 9.5 mL 0.05% SDS diluted to 70% with MilliQ water) using an Amicon ultracentrifugal filter (Merck Millipore). The buffer-exchanged samples were incubated at 70°C for 3 min, and then 1 μL of 20-fold diluted 10-kD Internal Standard (SCIEX, Framingham, MA) in MilliQ water was added to make a total volume of 70 μL. A PA800 Plus Pharmaceutical Analysis CE system (SCIEX) equipped with a PDA (Photodiode Array) detector at 214 nm and 32 Karat software (v.10.3 Build 20, SCIEX) was used for all experiments. A bare fused-silica capillary (50 μm I.D., 30 cm total length, 20 cm effective length, SCIEX) was used for separation. Data acquisition and analysis were performed using 32 Karat software (v.10.3 Build 20, SCIEX).
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