Metagenomic DNA from fecal samples was extracted using the MO BIO PowerFecal DNA Isolation Kit (Qiagen) and stored in our repository at −80°C until the metagenomics analysis.15 (link) Samples were processed in an automated, high throughput manner using the QiaCube DNA/RNA Purification System (Qiagen) with bead beating in 0.1 mm glass bead plates. Isolated DNA was quantified and normalized using the Quant-iT Picogreen dsDNA Assay Kit. Shotgun metagenomic libraries were prepared with a procedure adapted from the Nextera Library Prep Kit (Illumina). Libraries were subsequently pooled and assessed using the Agilent Bioanalyzer. Sequencing was performed on either an Illumina NextSeq 550 (1 × 150 bp, NextSeq 500/550 High Output v2 kit) or an Illumina NovaSeq 6000 (1 × 100 bp, NovaSeq 6000 S2 Reagent Kit).
Qiacube dna rna purification system
Manufactured by Qiagen
The QiaCube DNA/RNA Purification System is a fully automated platform for the extraction and purification of nucleic acids (DNA and RNA) from various sample types. The system utilizes optimized reagents and protocols to provide reliable and consistent results.
Automatically generated - may contain errors
Lab products found in correlation
Nextseq 550, by Illumina (2 mentions)
Mo bio power fecal dna isolation kit, by Qiagen (2 mentions)
Nextseq 500 550 high output kit v2, by Illumina (2 mentions)
Novaseq 6000 s2 reagent kit, by Illumina (2 mentions)
Agilent bioanalyzer, by Agilent Technologies (2 mentions)
Novaseq 6000, by Illumina (2 mentions)
Nextera library prep kit, by Illumina (2 mentions)
2 protocols using qiacube dna rna purification system
1
Metagenomic DNA Extraction and Sequencing Protocol
2
High-Throughput Fecal Metagenome Sequencing
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Metagenomic DNA from fecal samples was extracted using the MO BIO PowerFecal DNA Isolation Kit (Qiagen). Samples were processed in an automated high‐throughput manner using the QiaCube DNA/RNA Purification System (Qiagen) with bead beating in 0.1‐mm glass‐bead plates. Isolated DNA was quantified and normalized using the Quant‐iT Picogreen Double‐Stranded DNA Assay Kit. Shotgun metagenomic libraries were prepared with a procedure adapted from the Nextera Library Prep Kit (Illumina). Libraries were subsequently pooled and assessed using the Agilent Bioanalyzer. Sequencing was performed on either an Illumina NextSeq 550 (1 × 150 base pairs [bp]; NextSeq 500/550 High Output v2 kit) or an Illumina NovaSeq 6000 (1 × 100 bp; NovaSeq 6000 S2 Reagent Kit).
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