Su5416

miRNA-30a KO mice were obtained under license from Nanjing Biomedical Research Institute of Nanjing University (project no. XM001304). miR-30a KO mice or age-matched WT controls (strain C57BL/6, 8 weeks of age) were randomly divided into 4 groups: (1) a vehicle-normoxia-treated WT group (WT+Nor/Veh) (n = 10); (2) a Su5416-hypoxia-treated WT group (WT+Su/Hx) (n = 10); (3) a vehicle-normoxia-treated miR-30a^−/− group (miR-30a KO+Nor/Veh) (n = 6); and (4) a Su5416-hypoxia-treated miRNA-30a^−/− group (miR-30a KO+Su/Hx) (n = 8). The WT+Su/Hx and miR-30a KO+Su/Hx groups were injected subcutaneously with Su5416 (20 mg/kg, TargetMol, Boston, MA, USA) weekly, which was suspended in CMC (0.5% [w/v] carboxymethylcellulose sodium, 0.9% [w/v] sodium chloride, 0.4% [v/v] polysorbate 80, 0.9% [v/v] benzyl alcohol in deionized water) as previously described.⁴² (link) Then, the animals were exposed to chronic normobaric hypoxia (10% O₂) in a chamber for 21 days. The WT+Nor/Veh and miR-30a KO+Nor/Veh groups received only vehicle, and then the animals were kept in room air. At study termination, mice were anesthetized and sacrificed. A schematic of the experimental design is illustrated in Figure S1A.