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Anacardic acid

Manufactured by Cayman Chemical
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Anacardic acid is a naturally occurring organic compound that is commonly extracted from the shell of cashew nuts. It functions as an antioxidant and has been studied for its potential biological activities.

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Lab products found in correlation

Paragyline, by Cayman Chemical (2 mentions) Ms 275, by Merck Group (2 mentions) Rgfp966, by Selleck Chemicals (2 mentions) 5 azacytidine, by Cayman Chemical (2 mentions) Tmp269, by Selleck Chemicals (2 mentions) Pci 34051, by Selleck Chemicals (2 mentions) Importazole, by Cayman Chemical (1 mentions) Cytochalasin d, by Cayman Chemical (1 mentions) Hdac activity inhibition assay kit, by Epigentek (1 mentions) Nuclear extraction kit, by Epigentek (1 mentions) Garcinol, by Cayman Chemical (1 mentions) Histone h4 antibody, by Abcam (1 mentions) Goat anti mouse igg hrp, by Santa Cruz Biotechnology (1 mentions) Anti flag affinity gel, by Merck Group (1 mentions) Acetyl histone h3 antibody, by Cell Signaling Technology (1 mentions) Acetyl histone h4 antibody, by Cell Signaling Technology (1 mentions) Curcumin, by Cayman Chemical (1 mentions) Protease inhibitor cocktail, by Merck Group (1 mentions) Ecl plus western blotting detection reagent, by Thermo Fisher Scientific (1 mentions) Universal nuclease for cell lysis, by Thermo Fisher Scientific (1 mentions)

4 protocols using anacardic acid

1

Mechanotransduction and Epigenetic Regulation

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Nuclear protein extractions were isolated from 105 fibroblasts cultured on glass, PAAm, MeHA, or o-NB PAAm gels of varying stiffness for the indicated time points by using the nuclear extraction kit (EpiGentek, OP-0002, USA), according the manufacturer’s instructions. HAT and HDAC activity were measured by using the HAT activity/inhibition assay kit (EpiGentek, P-4003-48, USA) and HDAC activity/inhibition assay kit (EpiGentek, P-4034-96, USA), respectively. Following the manufacturer’s instructions, 5 μg of nuclear extract was added into the assay wells and incubated at 37°C for 60 min. After adding the color developer solution, the absorbance was measured using a plate reader (Infinite 200Pro, 30050303) at 450 nm. For inhibitor experiments, fibroblasts were cultured on glass and PA gels of varying stiffness for 1 day, followed by treatment with vehicle control (DMSO), anacardic acid (1 μM; Cayman chemical, 13144), VPA (500 μM; Cayman chemical, 13033), cytochalasin D (0.5 μM or indicated concentrations; Cayman chemical, 11330), importazole (10 μM; Cayman chemical, 21491), or BMS-5 (4 μM; Cayman chemical, 21072) for 24 hours before isolating nuclear protein extracts and measuring HAT or HDAC activity as aforementioned.
Song Y., Soto J., Wong S.Y., Wu Y., Hoffman T., Akhtar N., Norris S., Chu J., Park H., Kelkhoff D.O., Ang C.E., Wernig M., Kasko A., Downing T.L., Poo M.M, & Li S. (2024). Biphasic regulation of epigenetic state by matrix stiffness during cell reprogramming. Science Advances, 10(7), eadk0639.
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2

HDAC Inhibition and TNF-α Secretion Assay

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For the HDAC inhibition assay, cells were pretreated with TSA (5nM, Sigma-Aldrich). For the TNF-α secretion assay, human THP1 or murine macrophage RAW264.7 cells were treated with TSA, VPA (0.1 μM), Ms275 (5 μM), NIC (0.5 μM) HPOB (0.5 μM) (Sigma-Aldrich), TMP269 (0.1 μM), PCI-34051 (0.1 μM), RGFP966 (0.5 μM) (Selleckchem), paragyline (3 μM), 5'azacytidine (10 μM) or anacardic acid (10 μM) (Cayman Chemical). The concentration of HDAC inhibitors used did not induce cytotoxicity (Supplementary Fig.5 a-h). It is important to note that at high concentrations, HDAC inhibitors blocked TNF-α secretion (Supplementary Fig. 7b-i) 30 (link).
Bandyopadhaya A., Tsurumi A., Maura D., Jeffrey K.L, & Rahme L.G. (2016). A Quorum Sensing Signal Promotes Host Tolerance Training Through HDAC1-Mediated Epigenetic Reprogramming. Nature microbiology, 1, 16174.
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3

Biochemical Protocols for Protein Purification

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Commercially available Curcumin (#81025.1), Anacardic acid (#13144), Garcinol (#10566) and IsoGarcinol (#21164) were purchased from Cayman. Anti-Flag affinity gel (#A2220) and anti-Flag antibody conjugated with horseradish peroxidase (#A8592) were purchased from Sigma-Aldrich. Acetyl-Histone H3 Antibody (#9675) and acetyl histone H4 antibody (#13534) were purchased from Cell Signaling Technology. SHMT2 (mSHMT Antibody (F-11): sc-390641- Non-conjugated), ß-actin antibody (SC-47778 HRP) and goat anti-mouse IgG-HRP (sc-2005) were purchased from Santa Cruz. And histone H4 antibody (#ab197517) was purchased from Abcam. Triple Flag peptide for eluting Flag-tagged HDAC11 enzyme and protease inhibitor cocktail were purchased from Sigma-Aldrich. ECL plus western blotting detection reagent and universal nuclease for cell lysis were purchased from Thermo Scientific Pierce. Acyl peptides (myristoyl-H3K9) and Alk14 were synthesized.26 (link) The peptide sequence for the H3K9 peptide is KQTARK(myristoyl)STGGWW with uncapped N-terminal and SHMT2 peptide is SDEVK(myristoyl)AHLLAWW with capped acetyl N-terminal.
Son S.I., Su D., Ho T.T, & Lin H. (2020). Garcinol Is an HDAC11 Inhibitor. ACS chemical biology, 15(11), 2866-2871.
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4

HDAC Inhibition and TNF-α Secretion Assay

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For the HDAC inhibition assay, cells were pretreated with TSA (5nM, Sigma-Aldrich). For the TNF-α secretion assay, human THP1 or murine macrophage RAW264.7 cells were treated with TSA, VPA (0.1 μM), Ms275 (5 μM), NIC (0.5 μM) HPOB (0.5 μM) (Sigma-Aldrich), TMP269 (0.1 μM), PCI-34051 (0.1 μM), RGFP966 (0.5 μM) (Selleckchem), paragyline (3 μM), 5'azacytidine (10 μM) or anacardic acid (10 μM) (Cayman Chemical). The concentration of HDAC inhibitors used did not induce cytotoxicity (Supplementary Fig.5 a-h). It is important to note that at high concentrations, HDAC inhibitors blocked TNF-α secretion (Supplementary Fig. 7b-i) 30 (link).
Bandyopadhaya A., Tsurumi A., Maura D., Jeffrey K.L, & Rahme L.G. (2016). A Quorum Sensing Signal Promotes Host Tolerance Training Through HDAC1-Mediated Epigenetic Reprogramming. Nature microbiology, 1, 16174.
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