Dako envision flex plus mouse link kit

Manufactured by Agilent Technologies

Sourced in United States

The Dako Envision Flex Plus Mouse Link Kit is a laboratory equipment product designed for use in immunohistochemistry (IHC) and in situ hybridization (ISH) procedures. It is intended to enhance the visualization of target antigens or nucleic acid sequences in biological samples.

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Lab products found in correlation

Dako autostainer link 48, by Agilent Technologies (2 mentions) Aperio at2 scanner, by Leica (1 mentions)

Close spelling variants of this product

Dako Envision Flex Kit Dako envision plus kit Dako EnVision Flex Envision Flex Plus kit DAKO Envision kit ENVISION FLEX Mouse+, EnVision FLEX kit Envision Plus kit EnVision FLEX Envision Plus

2 protocols using dako envision flex plus mouse link kit

Immunohistochemical Analysis of CRMP4 in Human Spinal Cord

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The Dako Autostainer Link 48 (Agilent, USA) was used for all human spinal cord immunohistochemistry. The CRMP4 antibody (Millipore AB5454) was used at 1:700 for 30 min at room temperature. Heat‐induced epitope retrieval was used prior to staining with Dako’s EnV Flex Low pH TRS. The Dako Envision Flex Plus Mouse Link Kit (Agilent, USA) to detect the antibody along with the Dako DAB (Agilent, USA). CRMP4 relative expression was semi‐quantified by scoring IHC spinal cord sections between 1 and 3, blindly: 1 = low expression, 2 = middle expression, and 3 = high expression.

Maimon R., Ankol L., Gradus Pery T., Altman T., Ionescu A., Weissova R., Ostrovsky M., Tank E., Alexandra G., Shelestovich N., Opatowsky Y., Dori A., Barmada S., Balastik M, & Perlson E. (2021). A CRMP4‐dependent retrograde axon‐to‐soma death signal in amyotrophic lateral sclerosis. The EMBO Journal, 40(17), e107586.

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Immunostaining and Whole-Slide Imaging Protocol

Cited 1 time

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Immunostaining was accomplished using the Dako Autostainer Link 48 (Agilent, USA). Anti-YTHDF2 antibody (Proteintech, #24744-1-AP, 1:300) or anti-m6A antibody (Synaptic Systems #202003, 1:100) were used with the Dako High pH Target Retrieval Solution (Tris/EDTA, pH 9; Agilent, USA) (20 minutes, 97°C) and the Dako Envision Flex Plus Mouse Link Kit (Agilent, USA) to detect the antibody along with the Dako DAB (Agilent, USA). Whole-slide images were generated by the University of Michigan Digital Pathology group within the Department of Pathology using a Leica Biosystems Aperio AT2 scanner equipped with a 0.75 NA Plan Apo 20x objective; 40x scanning is achieved using a 2x optical magnification changer. Resolution is 0.25 microns per pixel for 40x scans. Focus during the scan is maintained using a triangulated focus map built from individual focus points determined in a separate step before scanning is started. Proprietary software is used for image processing during acquisition. Image analysis performed using QuPath software^{91 (link)} and mean intensity calculated for stained cells.

McMillan M., Gomez N., Hsieh C., Bekier M., Li X., Miguez R., Tank E.M, & Barmada S.J. (2023). RNA methylation influences TDP43 binding and disease pathogenesis in models of amyotrophic lateral sclerosis and frontotemporal dementia. Molecular cell, 83(2), 219-236.e7.

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