LAD2 cells (3 × 105) were incubated with Spike-RBD protein (5 μg/mL, Genscript, Z03483) in adherent buffer (1 mM CaCl2, 2 mM MgCl2 and 5% BSA, pH 7.4) for 1 h at 4 °C. The cells were then fixed with 4% paraformaldehyde (Sigma–Aldrich) for 30 min at room temperature and stained with anti-His-tag antibodies (Abmart, M30111S). Subsequently, the cells were stained with goat anti-mouse Alexla Fluor 488-conjugated secondary antibodies (Invitrogen, A11001), and were detected with flow cytometry (BD Accuri C6) and analyzed with the FlowJo 7.6.1 software. In some experiments, LAD2 cells were prior-blocked with anti-ACE2 antibody (5 μg/mL, R&D Systems, AF933) for 1 h at 37 °C before the incubation with Spike-RBD protein.
Goat anti mouse alexla fluor 488 conjugated secondary antibodies
Manufactured by Thermo Fisher Scientific
Goat anti-mouse Alexa Fluor 488-conjugated secondary antibodies are fluorescently-labeled antibodies that bind to mouse primary antibodies. They are used in immunoassays and other applications to detect and visualize target proteins.
Automatically generated - may contain errors
2 protocols using goat anti mouse alexla fluor 488 conjugated secondary antibodies
1
Spike-RBD Protein Binding to LAD2 Cells
2
Spike Protein Binding to LAD2 Cells
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LAD2 cells (3×10 5 ) were incubated with Spike-RBD protein (5 μg/mL, Genscript, Z03483) in adherent buffer (1mM CaCl 2 , 2mM MgCl 2 and 5% BSA, pH 7.4) for 1h at 4 °C. The cells were then fixed with 4% paraformaldehyde (Sigma-Aldrich) for 30 min at room temperature and stained with anti-His-tag antibodies (Abmart, M30111S). Subsequently, the cells were stained with goat anti-mouse Alexla Fluor 488-conjugated secondary antibodies (Invitrogen, A11001), and were detected with flow cytometry. In some experiments, LAD2 cells were prior-treated with 0.25% trypsin (without EDTA) for 10 min at 37 °C or prior-blocked with anti-ACE2 antibody (5 μg/mL, R&D Systems, AF933) for 2 h at 37 °C before the incubation with Spike-RBD protein.
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