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Stemsep human b cell enrichment kit

Manufactured by STEMCELL
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Sourced in Canada

The StemSep® Human B Cell Enrichment kit is a laboratory product designed to isolate human B cells from biological samples. It utilizes immunomagnetic particles to separate B cells from other cell types, allowing for their purification and subsequent analysis or experimentation.

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Lab products found in correlation

Lsrii flow cytometer, by BD (1 mentions)

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StemSep kit (3 citations)

2 protocols using stemsep human b cell enrichment kit

1

Purification of Human B Cells

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Human B cells were purified from freshly isolated peripheral blood mononuclear cells using the StemSep® Human B Cell Enrichment kit according to the manufacturer’s instructions (StemCell Technologies). The purity of the CD19+ fraction was typically >95% and was determined by CD19 expression by FACS.
Rozmus J., Kariminia A., Abdossamadi S., Storer B.E., Martin P.J., Lee S.J., Wolff D., Arora M., Cutler C, & Schultz K.R. (2018). Comprehensive B Cell Phenotyping Profile for Chronic Graft-Versus-Host Disease Diagnosis. Biology of blood and marrow transplantation : journal of the American Society for Blood and Marrow Transplantation, 25(3), 451-458.
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2

Isolation and Purification of Human B Cells

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Human B cells were purified after isolation of PBMCs as described above by negative selection using the StemSep® Human B Cell Enrichment kit according to the manufacturer’s instructions (StemCell Technologies, Vancouver, Canada). The purity of the CD19+ fraction was > 95% as determined by CD19 expression by FACS on an LSRII flow cytometer (BD Biosciences).
Novice T., Kariminia A., Del Bel K.L., Lu H., Sharma M., Lim C.J., Read J., Lugt M.V., Hannibal M.C., O’Dwyer D., Hosler M., Scharnitz T., Rizzo J.M., Zacur J., Priatel J., Abdossamadi S., Bohm A., Junker A., Turvey S.E., Schultz K.R, & Rozmus J. (2019). A Germline Mutation in the C2 Domain of PLCγ2 Associated with Gain-of-Function Expands the Phenotype for PLCG2-Related Diseases. Journal of Clinical Immunology, 40(2), 267-276.
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