Immunohistochemical staining was carried out according to a standard method. 3-μm tissue sections were deparaffinised in xylene and rehydrated through a graded alcohol series. Heating in a microwave oven in a target retrieval solution pH 9.0 (TRS High pH; Dako) for 30 min was used for antigen retrieval. Endogenous peroxidase activity was quenched with 0.3% hydrogen peroxide in methanol for 30 min. The sections were washed with TBS and incubated with primary antibodies against: Bmi-1 (Invitrogen, USA, dilution 1 : 600, Catalog number PA5-23308), and CK15 (Invitrogen, USA, dilution 1 : 400, Catalog number MA1-90926), Bcl-2 Oncoprotein (Dako, RTU- Flex, clone 124, Catalog number IR 614). After washing, an adequate EnVision-HRP detection system (Dako, Carpinteria, CA, USA) was used. 3,3’-diaminobenzidine was used as the chromogen. After counterstaining with Mayer’s haematoxylin, the slides were washed, dehydrated, cleared in xylene and coverslipped. Negative controls for immunohistochemical staining were prepared with primary antibodies replaced by the antibody diluent.
Bcl 2 oncoprotein
Manufactured by Agilent Technologies
Sourced in Denmark
The Bcl-2 Oncoprotein is a laboratory equipment product designed for research purposes. It is a protein involved in the regulation of apoptosis, a process of programmed cell death. The Bcl-2 Oncoprotein is a key component in the study of cellular mechanisms and plays a role in various areas of biological research.
Automatically generated - may contain errors
Lab products found in correlation
Envision hrp detection system, by Agilent Technologies (1 mentions)
Trs high ph, by Agilent Technologies (1 mentions)
Mum1p, by Agilent Technologies (1 mentions)
Mib 1, by Agilent Technologies (1 mentions)
Ber h2, by Agilent Technologies (1 mentions)
C myc, by Abcam (1 mentions)
Sp 350 digital camera, by Olympus (1 mentions)
3 protocols using bcl 2 oncoprotein
1
Immunohistochemical Staining for Bmi-1, CK15, and Bcl-2
2
Immunohistochemical Analysis of Lymphoid Neoplasms
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Excised tissue specimens were fixed in 10% formalin and embedded in paraffin wax. Serial sections from the paraffin blocks were stained with hematoxylin and eosin. Immunohistochemical analysis was performed according to standard procedures. The antibodies used are as follows: CD3 (1:50, PS1; Leica Biosystems, Newcastle upon Tyne, UK), CD5 (1:50, 4C7; Leica Biosystems), CD10 (1:50, 56C6; Leica Biosystems), CD20 (1:100, L26; Leica Biosystems), CD30 (1:30, Ber-H2; Dako, Glostrup, Denmark), BCL2 oncoprotein (1:50, 124; Dako), BCL6 oncoprotein (1:100, LN22, Leica Biosystems), multiple myeloma oncogene 1 (MUM1; 1:50, MUM1p; Dako), cyclin D1 (1:100, DCS-6; Nichirei Biosciences Inc., Tokyo, Japan), Ki-67 (1:100, MIB-1; Dako), and c-MYC (1:200, Y69; Abcam plc., Cambridge, UK). Positivity was evaluated using cut-off values of 40% for MYC and 20% for others.
3
Evaluating Protective Effects of HDDPiW-jSB on Hair Follicles
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The hair skin samples were xed in 10% neutral-buffered formalin solution (Chempur, Poland), treated with graded alcohol, xylol series, and blocked-in para n (Histowax, Histo-Lab. Ltd, Goteborg, Sweden). Obtained 4 μm sections of skin from para n blocks were stained according to the hematoxylin-eosin (H&E) method. These sections were evaluated under the microscope and images were obtained (Olympus Stream Start BX 51, Olympus SP 350 digital camera, Japan).
On the rst stage of study, the dose response of 10 mg/kg and 30 mg/kg DTX on hair follicles were evaluated. Then, the study was continued with 10 mg/kg DTX in order to reveal and interpret the possible protective effects of HDDPiW-jSB solution. Tissues were stained with H&E and follicles counted as anagen, catagen, telogen and terminal hair follicles in ve areas (x40) of each sample. Next, immunohistopathological staining of CD34 (Clone QBEnd10, Dako, Denmark), Bcl-2 oncoprotein (b cell leukemia protein-2) (Clone 124, Dako, Denmark), apoptosis-related cysteine protease (caspase 3) (mouse monoclonal antibody, Vision Biosystems Novocastra, Newcastle, United Kingdom) were performed in three areas of each sample (x400). Olympus Stream Start BX51 microscope and Image J software were used for the analyses.
On the rst stage of study, the dose response of 10 mg/kg and 30 mg/kg DTX on hair follicles were evaluated. Then, the study was continued with 10 mg/kg DTX in order to reveal and interpret the possible protective effects of HDDPiW-jSB solution. Tissues were stained with H&E and follicles counted as anagen, catagen, telogen and terminal hair follicles in ve areas (x40) of each sample. Next, immunohistopathological staining of CD34 (Clone QBEnd10, Dako, Denmark), Bcl-2 oncoprotein (b cell leukemia protein-2) (Clone 124, Dako, Denmark), apoptosis-related cysteine protease (caspase 3) (mouse monoclonal antibody, Vision Biosystems Novocastra, Newcastle, United Kingdom) were performed in three areas of each sample (x400). Olympus Stream Start BX51 microscope and Image J software were used for the analyses.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!