Rats received an injection (IP) of [3H](2R,6R)-HNK (2 μCi/g), at the indicated time point and brain, blood, and tissues were collected for radiometric analyses. The brains were flash frozen in isopentane (Sigma-Aldrich) and stored at −80°C until use. The blood was collected and after clotting centrifuged (13,000 rpm, 10 min at RT) and serum was collected. The tissues were solubilized with Solvable™ (PerkinElmer) and bleached with hydrogen peroxide (Sigma-Aldrich). Serum and tissue samples were dissolved in scintillation cocktail and radioactivity counts were determined in a Beckman LS 60000TA scintillation counter (BeckmanCoulter, Indianapolis, IN, USA). The brains were flash frozen in isopentane. Then the brain tissue was sectioned (10 μm) on a cryostat (Leica, Germany) and thaw mounted onto ethanol-washed glass slides. Slides were air dried and placed in a Hypercassette™ (Amersham Biosciences) and covered with a BAS-TR2025 Storage Phosphor Screen (FujiFilm, Japan). The slides were exposed to the screen for 10–14 days and imaged using a phosphorimager (Typhoon FLA 7000; GE Healthcare).
Bas tr2025 storage phosphor screen
Manufactured by Fujifilm
Sourced in Japan
The BAS-TR2025 Storage Phosphor Screen is a piece of lab equipment designed for the storage and readout of X-ray and radiation data. It utilizes storage phosphor technology to capture and store images, which can then be scanned and digitized for further analysis.
Automatically generated - may contain errors
3 protocols using bas tr2025 storage phosphor screen
1
Pharmacokinetics of Radiolabeled Compound
2
Radiolabeled Ligand Binding Assay
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Flash frozen tissue (both rodents and the monkey) was sectioned (20 µm) on a cryostat (Leica, Germany) and thaw mounted onto ethanol-washed glass slides. Slides were pre-incubated (10 min, RT) in incubation buffer (50 mM Tris-HCl pH 7.4 with 10 mM of MgCl2), then slides were incubated (60 min) in incubation buffer containing [3H]clozapine (3.5 nM), [3H]C21 (10 nM, 41 Ci/mmol, Novandi, Sweden) or [3H]C13 (3.5 nM, 13 Ci mmol−1, Novandi, Sweden) with or without increasing amounts of the indicated cold ligands (Tocris (clozapine, C21) or custom synthesis). Slides were air dried and placed in a Hypercassette™ (Amersham Biosciences) and covered with a BAS-TR2025 Storage Phosphor Screen (FujiFilm, Japan). The slides were exposed to the screen for 5–7 days and imaged using a phosphor imager (Typhoon FLA 7000; GE Healthcare).
3
Biodistribution of Radiolabeled HNK
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Rats received an injection (IP) of [ 3 H](2R,6R)-HNK (2 μCi/g), at the indicated time point and brain, blood, and tissues were collected for radiometric analyses. The brains were flash frozen in isopentane (Sigma-Aldrich) and stored at -80°C until use. The blood was collected and after clotting centrifuged (13,000 rpm, 10 min at RT) and serum was collected. The tissues were solubilized with Solvable ™ (PerkinElmer) and bleached with hydrogen peroxide (Sigma-Aldrich). Serum and tissue samples were dissolved in scintillation cocktail and radioactivity counts were determined in a Beckman LS 60000TA scintillation counter (BeckmanCoulter, Indianapolis, IN, USA). The brains were flash frozen in isopentane. Then the brain tissue was sectioned (10 μm) on a cryostat (Leica, Germany) and thaw mounted onto ethanol-washed glass slides. Slides were air dried and placed in a Hypercassette ™ (Amersham Biosciences) and covered with a BAS-TR2025 Storage Phosphor Screen (FujiFilm, Japan). The slides were exposed to the screen for 10-14 days and imaged using a phosphorimager (Typhoon FLA 7000; GE Healthcare).
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