Log In Sign up
The largest database of trusted experimental protocols

Multiprobe

Manufactured by PerkinElmer
Visit Supplier

The MultiProbe is a versatile liquid handling system designed for automated sample preparation and assay processing in life science laboratories. It features multiple pipetting channels and a modular design to handle a variety of microplate formats and liquid handling tasks with precision and efficiency.

Automatically generated - may contain errors

Lab products found in correlation

Multiprobe liquid, by PerkinElmer (2 mentions) Genios, by Tecan (1 mentions) Sequencing grade modified porcine trypsin, by Promega (1 mentions)

Spelling variants of this product

MultiProbe II (12 citations) Multiprobe II Plus HTSEX robot (2 citations) MultiProbe liquid (2 citations) Multiprobe II plus liquid handling system (2 citations) Multiprobe II EX Robotic Liquid Handling System (2 citations)

4 protocols using multiprobe

1

Pooled Deletion Strain Growth Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
Pooled deletion strains were diluted to starting OD600 of 0.0625 in 700 μl and were grown in duplicate wells on the same plate for five doublings in a Tecan Genios (Tecan Systems, Inc.) spectrophotometer at 30°C. Cells were manually harvested (synthetic media and drug screens) or automatically collected (YPG screens) using a Packard Multiprobe (PerkinElmer) liquid handler and stored at −20°C in a 48-well plate for no longer than 1 day. For the amino acid dropout experiments, each pooled collection was grown in SC medium or rich medium (YPD) as the control condition, and synthetic medium with an individual amino acid of interest dropped out as the experimental condition. For the YPG experiments, 3% glycerol was the experimental condition and YPD was the control condition.
Acton E., Lee A.H., Zhao P.J., Flibotte S., Neira M., Sinha S., Chiang J., Flaherty P., Nislow C, & Giaever G. (2017). Comparative functional genomic screens of three yeast deletion collections reveal unexpected effects of genotype in response to diverse stress. Open Biology, 7(6), 160330.
+ Open protocol
+ Expand
2

Tryptic Digestion and Mass Spectrometry

Check if the same lab product or an alternative is used in the 5 most similar protocols
Bands of interest were extracted from gels and placed in 96-well plates and then washed with water. Tryptic digestion was performed on a liquid handling robot (MultiProbe, Perkin Elmer) according to the manufacturer’s specifications. Briefly, proteins were reduced with 10 mM DTT and alkylated with 55 mM iodoacetamide. Trypsin digestion was performed using 126 nM of modified porcine trypsin (Sequencing grade, Promega, Madison, WI) at 37°C for 18 h. Digestion products were extracted using 1% formic acid, 2% acetonitrile followed by 1% formic acid, 50% acetonitrile. The recovered extracts were pooled, vacuum-centrifuge-dried and then resuspended into 12 µL of 0.1% formic acid, and 5 µL was analysed by mass spectrometry. Protein digestion and mass spectrometry analyses were performed by the Proteomics Platform of the CHU de Québec Research Center (Quebec, Qc, Canada).
Benmoussa A., Ly S., Shan S.T., Laugier J., Boilard E., Gilbert C, & Provost P. (2017). A subset of extracellular vesicles carries the bulk of microRNAs in commercial dairy cow’s milk. Journal of Extracellular Vesicles, 6(1), 1401897.
+ Open protocol
+ Expand
3

High-Throughput DNA Library Quantification

Check if the same lab product or an alternative is used in the 5 most similar protocols
After in-solution hybrid selection, libraries were quantified using qPCR (KAPA Biosystems) with probes specific to the ends of the adapters. This assay was automated using Agilent’s Bravo liquid handling platform. Based on qPCR quantification, libraries were normalized to 2nM and then denatured using 0.1 N NaOH using Perkin-Elmer’s MultiProbe liquid handling platform. A subset of the samples prepared using forked, indexed adapters was quantified using qPCR, normalized to 2nM using Perkin-Elmer’s Mini-Janus liquid handling platform, and pooled by equal volume using the Agilent Bravo. Pools were then denatured using 0.1 N NaOH. Denatured samples were diluted into strip tubes using the Perkin-Elmer MultiProbe.
Do R., Stitziel N.O., Won H.H., Jørgensen A.B., Duga S., Merlini P.A., Kiezun A., Farrall M., Goel A., Zuk O., Guella I., Asselta R., Lange L.A., Peloso G.M., Auer P.L., Girelli D., Martinelli N., Farlow D.N., DePristo M.A., Roberts R., Stewart A.F., Saleheen D., Danesh J., Epstein S.E., Sivapalaratnam S., Hovingh G.K., Kastelein J.J., Samani N.J., Schunkert H., Erdmann J., Shah S.H., Kraus W.E., Davies R., Nikpay M., Johansen C.T., Wang J., Hegele R.A., Hechter E., Marz W., Kleber M.E., Huang J., Johnson A.D., Li M., Burke G.L., Gross M., Liu Y., Assimes T.L., Heiss G., Lange E.M., Folsom A.R., Taylor H.A., Olivieri O., Hamsten A., Clarke R., Reilly D.F., Yin W., Rivas M.A., Donnelly P., Rossouw J.E., Psaty B.M., Herrington D.M., Wilson J.G., Rich S.S., Bamshad M.J., Tracy R.P., Cupples L.A., Rader D.J., Reilly M.P., Spertus J.A., Cresci S., Hartiala J., Tang W.H., Hazen S.L., Allayee H., Reiner A.P., Carlson C.S., Kooperberg C., Jackson R.D., Boerwinkle E., Lander E.S., Schwartz S.M., Siscovick D.S., McPherson R., Tybjaerg-Hansen A., Abecasis G.R., Watkins H., Nickerson D.A., Ardissino D., Sunyaev S.R., O’Donnell C.J., Altshuler D., Gabriel S, & Kathiresan S. (2014). Multiple rare alleles at LDLR and APOA5 confer risk for early-onset myocardial infarction. Nature, 518(7537), 102-106.
+ Open protocol
+ Expand
4

High-Throughput DNA Library Quantification

Check if the same lab product or an alternative is used in the 5 most similar protocols
After in-solution hybrid selection, libraries were quantified using qPCR (KAPA Biosystems) with probes specific to the ends of the adapters. This assay was automated using Agilent’s Bravo liquid handling platform. Based on qPCR quantification, libraries were normalized to 2nM and then denatured using 0.1 N NaOH using Perkin-Elmer’s MultiProbe liquid handling platform. A subset of the samples prepared using forked, indexed adapters was quantified using qPCR, normalized to 2nM using Perkin-Elmer’s Mini-Janus liquid handling platform, and pooled by equal volume using the Agilent Bravo. Pools were then denatured using 0.1 N NaOH. Denatured samples were diluted into strip tubes using the Perkin-Elmer MultiProbe.
Do R., Stitziel N.O., Won H.H., Jørgensen A.B., Duga S., Merlini P.A., Kiezun A., Farrall M., Goel A., Zuk O., Guella I., Asselta R., Lange L.A., Peloso G.M., Auer P.L., Girelli D., Martinelli N., Farlow D.N., DePristo M.A., Roberts R., Stewart A.F., Saleheen D., Danesh J., Epstein S.E., Sivapalaratnam S., Hovingh G.K., Kastelein J.J., Samani N.J., Schunkert H., Erdmann J., Shah S.H., Kraus W.E., Davies R., Nikpay M., Johansen C.T., Wang J., Hegele R.A., Hechter E., Marz W., Kleber M.E., Huang J., Johnson A.D., Li M., Burke G.L., Gross M., Liu Y., Assimes T.L., Heiss G., Lange E.M., Folsom A.R., Taylor H.A., Olivieri O., Hamsten A., Clarke R., Reilly D.F., Yin W., Rivas M.A., Donnelly P., Rossouw J.E., Psaty B.M., Herrington D.M., Wilson J.G., Rich S.S., Bamshad M.J., Tracy R.P., Cupples L.A., Rader D.J., Reilly M.P., Spertus J.A., Cresci S., Hartiala J., Tang W.H., Hazen S.L., Allayee H., Reiner A.P., Carlson C.S., Kooperberg C., Jackson R.D., Boerwinkle E., Lander E.S., Schwartz S.M., Siscovick D.S., McPherson R., Tybjaerg-Hansen A., Abecasis G.R., Watkins H., Nickerson D.A., Ardissino D., Sunyaev S.R., O’Donnell C.J., Altshuler D., Gabriel S, & Kathiresan S. (2014). Multiple rare alleles at LDLR and APOA5 confer risk for early-onset myocardial infarction. Nature, 518(7537), 102-106.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!