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Neuro medium

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Neuro-medium is a cell culture medium designed for the growth and maintenance of primary neural cells, including neurons and glial cells. It provides the necessary nutrients and growth factors to support the specific requirements of neural cell cultures.

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Lab products found in correlation

Neurobrew 21, by Miltenyi Biotec (3 mentions) Nerve growth factor (ngf), by Inotiv (1 mentions) Glutamax, by Thermo Fisher Scientific (1 mentions) Poly l lysine (pll), by Merck Group (1 mentions) Laminin, by Merck Group (1 mentions)

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MACS Neuro Medium (27 citations)

5 protocols using neuro medium

1

Murine DRG Explant Culture for Axon Degeneration

Cited 1 time
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Murine DRG explants dissected from embryonic day (E) 13 C56BL/6J mice were cultured on poly-L-lysine (PLL) and laminin-coated 24-well plates in Neuro-medium (Miltenyi Biotec) containing 10% FBS and 25 ng/ml nerve growth factor (NGF) (Harlan Bioproducts). After 24 h, the culture medium was changed to Neuro-medium supplemented with 2% Neuro-Brew-21 (Miltenyi Biotec), 25 ng/ml NGF, and 1 mM Glutamine in addition to a mixture of 1 mM 5′-fluoro-2′-deoxyuridine and 1 mM uridine to remove non-neuronal cells. The in vitro Wallerian degeneration of axons was introduced by removing cell bodies at 10–14 d in vitro using a pipette tip.5,6 (link)
Wakatsuki S, & Araki T. (2017). Specific phospholipid scramblases are involved in exposure of phosphatidylserine, an “eat-me” signal for phagocytes, on degenerating axons. Communicative & Integrative Biology, 10(2), e1296615.
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2

In Vitro Wallerian Degeneration of Murine DRG Axons

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Murine DRG explants dissected from embryonic day 13 C56BL/6J mice were cultured on poly-l-lysine– and laminin-coated 24-well plates in Neuro medium (Miltenyi Biotec) containing 10% FBS and 25 ng/ml NGF (Envigo). After 24 h, the culture medium was changed to Neuro medium supplemented with 2% Neuro-Brew-21 (Miltenyi Biotec), 25 ng/ml NGF, and 1 mM glutamine in addition to a mixture of 1 mM 5′-fluoro-2′-deoxyuridine and 1 mM uridine to remove nonneuronal cells. The in vitro Wallerian degeneration of axons was introduced by removing cell bodies at 10–14 d in vitro using a pipette tip (Wakatsuki et al., 2011 (link), 2015 (link)).
Wakatsuki S., Tokunaga S., Shibata M, & Araki T. (2017). GSK3B-mediated phosphorylation of MCL1 regulates axonal autophagy to promote Wallerian degeneration. The Journal of Cell Biology, 216(2), 477-493.
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3

Isolation and Phenotyping of Spinal Cord Cells

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Fresh spinal cord tissue was dissected following transcardial perfusion with PBS was weighed and placed in Neuro Medium (Miltenyi Biotec). Medium was removed and tissues were individually passed through 70 µm strainers, washed with 30% percoll to pellet cells and myelin-containing supernatant was carefully removed. Cells were resuspended in 200 µl flow cytometry staining buffer (FCSB; 1% FCS, 0.01% NaN3 in PBS) prior to cell surface staining with antibodies to CD45 (clone 104; 1:400), CD11b (clone M1/70; 1:400), CD3 (clone 145-2C11; 1:200) and CD4 (clone GK1.5; 1:200). Foxp3 expression was determined in fresh cells from Foxp3-eGFP or Foxp3-GFP-DTR mice. To calculate cell numbers, singlets were identified by FSC-H vs FSC-A, and CD45+ cells were gated for subsequent analyses of CD11b, CD3, CD4 and Foxp3-eGFP. All flow cytometry antibodies were from eBioscience. Data were acquired on a FACSCanto II and analyzed using FlowJo software.
Dombrowski Y., O’Hagan T., Dittmer M., Penalva R., Mayoral S.R., Bankhead P., Fleville S., Eleftheriadis G., Zhao C., Naughton M., Hassan R., Moffat J., Falconer J., Boyd A., Hamilton P., Allen I.V., Kissenpfennig A., Moynagh P.N., Evergren E., Perbal B., Williams A.C., Ingram R.J., Chan J.R., Franklin R.J, & Fitzgerald D.C. (2017). Regulatory T cells promote myelin regeneration in the Central Nervous System. Nature neuroscience, 20(5), 674-680.
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4

Isolation and Phenotyping of Spinal Cord Cells

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Fresh spinal cord tissue was dissected following transcardial perfusion with PBS was weighed and placed in Neuro Medium (Miltenyi Biotec). Medium was removed and tissues were individually passed through 70 µm strainers, washed with 30% percoll to pellet cells and myelin-containing supernatant was carefully removed. Cells were resuspended in 200 µl flow cytometry staining buffer (FCSB; 1% FCS, 0.01% NaN3 in PBS) prior to cell surface staining with antibodies to CD45 (clone 104; 1:400), CD11b (clone M1/70; 1:400), CD3 (clone 145-2C11; 1:200) and CD4 (clone GK1.5; 1:200). Foxp3 expression was determined in fresh cells from Foxp3-eGFP or Foxp3-GFP-DTR mice. To calculate cell numbers, singlets were identified by FSC-H vs FSC-A, and CD45+ cells were gated for subsequent analyses of CD11b, CD3, CD4 and Foxp3-eGFP. All flow cytometry antibodies were from eBioscience. Data were acquired on a FACSCanto II and analyzed using FlowJo software.
Dombrowski Y., O’Hagan T., Dittmer M., Penalva R., Mayoral S.R., Bankhead P., Fleville S., Eleftheriadis G., Zhao C., Naughton M., Hassan R., Moffat J., Falconer J., Boyd A., Hamilton P., Allen I.V., Kissenpfennig A., Moynagh P.N., Evergren E., Perbal B., Williams A.C., Ingram R.J., Chan J.R., Franklin R.J, & Fitzgerald D.C. (2017). Regulatory T cells promote myelin regeneration in the Central Nervous System. Nature neuroscience, 20(5), 674-680.
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5

Cortical Neuron Culture from Embryonic Mice

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Cerebral hemispheres were removed separately from embryonic day 14–16 C56BL/6J mice. Cells were dissociated with papain and seeded at a density of 4 × 105 cells/well onto 24-well plates coated with poly-L-lysine (Merck) and laminin (Merck) in DMEM containing 10% FBS. From the third day in vitro (DIV), the cells were maintained in Neuro-medium (Miltenyi Biotec) containing 2% Neuro-Brew-21 (Miltenyi Biotec) and 1 mM GlutaMAX (Thermo Fisher Scientific). The cells were cultured for 14–16 d and used for immunoblot or immunostaining experiments.
Wakatsuki S., Takahashi Y., Shibata M., Adachi N., Numakawa T., Kunugi H, & Araki T. (2021). Small noncoding vault RNA modulates synapse formation by amplifying MAPK signaling. The Journal of Cell Biology, 220(2), e201911078.
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