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6 aminonicotinamide

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6-aminonicotinamide is a chemical compound that can be used as a laboratory reagent. It is a colorless crystalline solid with the molecular formula C6H7N2O. This compound is commonly used in research and development applications.

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Lab products found in correlation

Chloroquine, by Merck Group (3 mentions) Hoechst, by Thermo Fisher Scientific (2 mentions) Etoposide, by Merck Group (2 mentions) Doxorubicin, by Merck Group (2 mentions) N acetylcysteine, by Merck Group (2 mentions) Phospho histone h3, by Cell Signaling Technology (2 mentions) Quinacrine, by Merck Group (2 mentions) Cleaved parp, by Abcam (2 mentions) Phospho p44 42 mapk p erk, by Thermo Fisher Scientific (2 mentions) P44 42 mapk erk1 2, by Thermo Fisher Scientific (2 mentions) Atg12, by Santa Cruz Biotechnology (2 mentions) Abc232, by Merck Group (2 mentions) 1 13c glucose, by Cambridge Isotopes (2 mentions) 1 14c glucose and 6 14c glucose, by PerkinElmer (2 mentions) Rapamycin, by Merck Group (2 mentions) N acetyl l cysteine nac, by Merck Group (2 mentions) 2 deoxy d glucose, by Merck Group (2 mentions) L glutamine 13c5, by Merck Group (2 mentions) Tween 80, by Merck Group (2 mentions) Trolox, by Merck Group (2 mentions)

Close spelling variants of this product

6-Aminonicotinamide (6-AN) (19 citations)

11 protocols using 6 aminonicotinamide

1

Immunoblotting and Immunofluorescence Analysis of Autophagy Markers

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Commercial antibodies include: phospho-Histone H3 (Cell Signaling Technology (CST) 9701), cleaved caspase-3 Alexa Fluor 488 (CST 9669S), phospho-p44/42 MAPK (P-ERK) (Invitrogen 44–680G), p44/42 MAPK (ERK1/2) (Invitrogen 13–6200), ATG12 (CST 2010), ATG7 (Santa Cruz Biotechnology sc8668), p62 (Progen Biotechnik GP62C), tubulin (Sigma T6199), p53 (DO1, Calbiochem OP43), cleaved PARP (CST 9451), G6PD (Abcam ab993) LC3 5F10 for IF (Axxora NT0231-00). For immunobloting, we utilized an LC3 antibody which has been described previously and is now commercially available (EMD Millipore ABC232) (35 (link)). Chemicals include: chloroquine (CQ), quinacrine (Q), 6-aminonicotinamide (6-AN), N-acetyl Cysteine (NAC), etoposide (Et) and doxorubicin (DX), all from Sigma-Aldrich, staurosporine (STS, EMD Chemicals), 1-13C glucose (Cambridge Isotopes), 1-14C glucose and 6-14C glucose (Perkin Elmer), and Hoechst (Invitrogen).
Salas E., Roy S., Marsh T., Rubin B, & Debnath J. (2015). Oxidative Pentose Phosphate Pathway Inhibition Is A Key Determinant of Antimalarial Induced Cancer Cell Death. Oncogene, 35(22), 2913-2922.
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2

Monocyte Training and Restimulation Assay

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100 μL cells were added to flat bottom 96-well plates. After washing with warm PBS, monocytes were incubated with culture medium only as a negative control or 5 μg/mL of β-glucan, (β-1,3-(D)-glucan, kindly provided by Professor David Williams), 100 μM mono methyl-fumarate, or dimethyl-2-oxoglutarate (Sigma) for 24 hr (in 10% pooled human serum). Cells were washed once with 200 μL warm PBS and incubated for 5 days in culture medium with 10% serum and medium was changed once. Cells were restimulated with 200 μL RPMI, Escherichia coli LPS (serotype 055:B5, Sigma-Aldrich, 10 ng/mL), or Pam3Cys (EMC Microcollections, L2000, 10 μg/mL). After 24 hr, supernatants were collected and stored at −20°C (see Figure S1). In some experiments, cells were preincubated (before β-glucan training) for 1 hr with 10 nM rapamycin (Sigma), 50 μM BPTES (Sigma), 2 μg/mL Ceruline (Sigma), 100 μM 6-aminonicotinamide (Sigma), and 20 μM fluvastatin sodium hydrate (Sigma). Concentrations were selected as being the highest non-cytotoxic concentrations.
Arts R.J., Novakovic B., ter Horst R., Carvalho A., Bekkering S., Lachmandas E., Rodrigues F., Silvestre R., Cheng S.C., Wang S.Y., Habibi E., Gonçalves L.G., Mesquita I., Cunha C., van Laarhoven A., van de Veerdonk F.L., Williams D.L., van der Meer J.W., Logie C., O’Neill L.A., Dinarello C.A., Riksen N.P., van Crevel R., Clish C., Notebaart R.A., Joosten L.A., Stunnenberg H.G., Xavier R.J, & Netea M.G. (2016). Glutaminolysis and Fumarate Accumulation Integrate Immunometabolic and Epigenetic Programs in Trained Immunity. Cell metabolism, 24(6), 807-819.
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3

Preparation of Compound Stock Solutions

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Solid stocks were purchased from the indicated suppliers and prepared as concentrated stock solutions in the appropriate solvent: hydroxyurea (100 mM in dH2O) from Acros, 2-deoxygluosce (1 M in dH2O), oxamate (100 mM in dH2O), 6-aminonicotinamide (2.5 mM in DMSO), piperlongumine (20 mM in DMSO), simvastin (20 mM in DMSO), L-buthionine-sulfoxamine (50 mM in dH2O) or chloroquine (20 mM in dH2O) from Sigma; GSK 2837808A (10 mM in DMSO) and metformin (100 mM in DMEM) from TOCRIS bioscience and TH588 (10 mM in DMSO) from Selleckchem. V158411 and VER-246008 were from Vernalis Research and prepared as 20 mM DMSO stocks. Compounds were serially diluted in the appropriate solvent to 500× or 1000× final concentration then to 5× or 10× final concentration in complete media before addition to cells to yield a 1× final concentration.
, & Massey A.J. (2017). Modification of tumour cell metabolism modulates sensitivity to Chk1 inhibitor-induced DNA damage. Scientific Reports, 7, 40778.
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4

Autophagy modulators in cell signaling

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BafA1 was obtained from Wako Chemicals or AdipoGen Life Sciences. Concanamycin A was obtained from Santa Cruz Biotechnology. Pepstatin A, E-64-d and leupeptin were obtained from Peptide Institute (Osaka, Japan). Wortmannin and MG-132 were obtained from Calbiochem. Ammonium chloride, novobiocin sodium salt, 3-MA, spautin-1 and 6-aminonicotinamide were obtained from Sigma. Recombinant mouse IL-1β was obtained from Cell Signaling Technology. The TLR2 ligand FSL-1 was obtained from InvivoGen. Other reagents were obtained from Wako Chemicals.
Into T., Horie T., Inomata M., Gohda J., Inoue J.I., Murakami Y, & Niida S. (2017). Basal autophagy prevents autoactivation or enhancement of inflammatory signals by targeting monomeric MyD88. Scientific Reports, 7, 1009.
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5

Reagents and Vectors for SLC7A11 and G6PD Studies

Cited 2 times
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SLC7A11 cDNA-containing expression vectors were described in previous publications 14 (link), 22 (link), 43 (link). G6PD shRNA constructs in GIPZ vector and G6PD cDNA were obtained from the Functional Genomics Core Facility of The University of Texas MD Anderson Cancer Center. G6PD cDNA was subsequently cloned into the lentivirus vector pCDH-hygromycin with C-terminal Myc tag. All constructs were confirmed by DNA sequencing. D-[1, 2-13C2] glucose (#GLC-026), 2-deoxy-D-[1-2H] glucose, and D-[3-2H] glucose (#GLC-034) were obtained from Omicro Biochemical. L-[1, 2, 1’, 2’−14C]-Cystine (#NEC854010UC) was from Perkine Elmer. Epiandrosterone (#CS-5183) was obtained from Chemscene. Following reagents were obtained from Sigma: BAY-876 (#SML1774–25MG), L-Glutamine-13C5 (#605166–100MG), N-Acetyl-L-Cysteine (NAC) (#A9165), 2-Deoxy-D-glucose (#D8375–1G), Sulfasalazine (#S0883–10G), D-Penicillamine (#P4875), L-Penicillamine (#196312), Trolox (#238813), 4-Hydroxy-TEMPO (#176141), Tris(2-carboxyethyl)phosphine hydrochloride(TCEP) (#C4706), 2-Mercaptoethanol (2ME) (#M6250), Deferoxamine mesylate salt (DFO) (#D9533), Ferrostatin-1 (#SML0583), 6-Aminonicotinamide (#A68203–1G), Methyl cellulose (#M0512–100G), TWEEN 80 (# P1754–500ML). All reagents were dissolved according to manufacturers’ instructions.
Liu X., Olszewski K., Zhang Y., Lim E.W., Shi J., Zhang X., Zhang J., Lee H., Koppula P., Lei G., Zhuang L., You M.J., Fang B., Li W., Metallo C.M., Poyurovsky M.V, & Gan B. (2020). Cystine transporter regulation of pentose phosphate pathway dependency and disulfide stress exposes a targetable metabolic vulnerability in cancer. Nature cell biology, 22(4), 476-486.
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6

Dunaliella tertiolecta Inhibition Assay

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Dunaliella tertiolecta UTEX 999 culture was obtained from UTEX. The inhibitors used in this study 3‐(3,4‐dichlorophenyl)‐1,1‐dimethylurea (DCMU; Catalog no: D2425), 2,5‐dibromo‐3‐methyl‐6‐isopropyl‐p‐benzoquinone (DBMIB; Catalog no: 71993), sodium malonate (Catalog no: 63409), 6‐aminonicotinamide (Catalog no: A68203), antimycin A (Catalog no: A8674), and aminooxyacetate (AOA; Catalog no: C13408‐1G) were all purchased from Sigma‐Aldrich. The oil used for preparation was a Macondo surrogate oil provided by BP Oil.
Kamalanathan M., Mapes S., Prouse A., Faulkner P., Klobusnik N.H., Hillhouse J., Hala D, & Quigg A. (2022). Core metabolism plasticity in phytoplankton: Response of Dunaliella tertiolecta to oil exposure. Journal of Phycology, 58(6), 804-814.
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7

Reagents and Vectors for SLC7A11 and G6PD Studies

Cited 2 times
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SLC7A11 cDNA-containing expression vectors were described in previous publications 14 (link), 22 (link), 43 (link). G6PD shRNA constructs in GIPZ vector and G6PD cDNA were obtained from the Functional Genomics Core Facility of The University of Texas MD Anderson Cancer Center. G6PD cDNA was subsequently cloned into the lentivirus vector pCDH-hygromycin with C-terminal Myc tag. All constructs were confirmed by DNA sequencing. D-[1, 2-13C2] glucose (#GLC-026), 2-deoxy-D-[1-2H] glucose, and D-[3-2H] glucose (#GLC-034) were obtained from Omicro Biochemical. L-[1, 2, 1’, 2’−14C]-Cystine (#NEC854010UC) was from Perkine Elmer. Epiandrosterone (#CS-5183) was obtained from Chemscene. Following reagents were obtained from Sigma: BAY-876 (#SML1774–25MG), L-Glutamine-13C5 (#605166–100MG), N-Acetyl-L-Cysteine (NAC) (#A9165), 2-Deoxy-D-glucose (#D8375–1G), Sulfasalazine (#S0883–10G), D-Penicillamine (#P4875), L-Penicillamine (#196312), Trolox (#238813), 4-Hydroxy-TEMPO (#176141), Tris(2-carboxyethyl)phosphine hydrochloride(TCEP) (#C4706), 2-Mercaptoethanol (2ME) (#M6250), Deferoxamine mesylate salt (DFO) (#D9533), Ferrostatin-1 (#SML0583), 6-Aminonicotinamide (#A68203–1G), Methyl cellulose (#M0512–100G), TWEEN 80 (# P1754–500ML). All reagents were dissolved according to manufacturers’ instructions.
Liu X., Olszewski K., Zhang Y., Lim E.W., Shi J., Zhang X., Zhang J., Lee H., Koppula P., Lei G., Zhuang L., You M.J., Fang B., Li W., Metallo C.M., Poyurovsky M.V, & Gan B. (2020). Cystine transporter regulation of pentose phosphate pathway dependency and disulfide stress exposes a targetable metabolic vulnerability in cancer. Nature cell biology, 22(4), 476-486.
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8

Metabolite Extraction and Quantification Workflow

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Methyl tertiary-butyl ether (MTBE) used for lipid and metabolite extraction was purchased from Sigma-Aldrich. LC-MS grade water, LC-MS grade acetonitrile (ACN), and HPLC grade isopropanol (IPA) were purchased from Fisher Chemical. HPLC grade methanol (MeOH) was purchased from Pharmco-Aaper. Mobile phase buffer LC-MS grade formic acid and formic acetate were purchased from Sigma-Aldrich. Fully carbon labeled 13C6 glucose for isotopic labeling was purchased from Cambridge Isotope Laboratories. High glucose DMEM and glucose-free Dulbecco’s modified eagle medium (DMEM) were purchased from Gibco. For the drug treatments, 6-aminonicotinamide, 2-deoxyglucose, compound 968 and rapamycin were purchased from Sigma-Aldrich. A Cadenza CD-C18 column (150 × 2 mm) was purchased from Imtakt, and an XBridge Amide HILIC column (3.5 µm, 4.6 × 100 mm) was purchased from Waters.
Huang H., Yuan M., Seitzer P., Ludwigsen S, & Asara J.M. (2020). IsoSearch: An Untargeted and Unbiased Metabolite and Lipid Isotopomer Tracing Strategy from HR-LC-MS/MS Datasets. Methods and Protocols, 3(3), 54.
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9

Metabolic Modulators in Cell Culture

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Gemcitabine, 5-azacytidine, decitabine, fialuridine, trifluorouridine, capecitabine, and 5-fluorouracil were sourced from Cayman Chemical. 6-Aminonicotinamide, 2-deoxyglucose, and all nucleosides and nucleobases were obtained from Sigma-Aldrich. Compounds were dissolved into PBS (Gibco) and added to media to treat cells. AZD7507 CSF1R has been previously described (Scott et al., 2013 (link)). FCCP, oligomycin, rotenone, antimycin A were from Sigma-Aldrich and stocks were prepared in DMSO; etomoxir, carnitine, palmitate, were from Sigma-Aldrich and prepared in water; BSA fraction V was from Roche and prepared in water.
Halbrook C.J., Pontious C., Kovalenko I., Lapienyte L., Dreyer S., Lee H.J., Thurston G., Zhang Y., Lazarus J., Sajjakulnukit P., Hong H.S., Kremer D.M., Nelson B.S., Kemp S., Zhang L., Chang D., Biankin A., Shi J., Frankel T.L., Crawford H.C., Morton J.P., di Magliano M.P, & Lyssiotis C.A. (2019). Macrophage-Released Pyrimidines Inhibit Gemcitabine Therapy in Pancreatic Cancer. Cell metabolism, 29(6), 1390-1399.e6.
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10

Immunoblotting and Immunofluorescence Analysis of Autophagy Markers

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Commercial antibodies include: phospho-Histone H3 (Cell Signaling Technology (CST) 9701), cleaved caspase-3 Alexa Fluor 488 (CST 9669S), phospho-p44/42 MAPK (P-ERK) (Invitrogen 44–680G), p44/42 MAPK (ERK1/2) (Invitrogen 13–6200), ATG12 (CST 2010), ATG7 (Santa Cruz Biotechnology sc8668), p62 (Progen Biotechnik GP62C), tubulin (Sigma T6199), p53 (DO1, Calbiochem OP43), cleaved PARP (CST 9451), G6PD (Abcam ab993) LC3 5F10 for IF (Axxora NT0231-00). For immunobloting, we utilized an LC3 antibody which has been described previously and is now commercially available (EMD Millipore ABC232) (35 (link)). Chemicals include: chloroquine (CQ), quinacrine (Q), 6-aminonicotinamide (6-AN), N-acetyl Cysteine (NAC), etoposide (Et) and doxorubicin (DX), all from Sigma-Aldrich, staurosporine (STS, EMD Chemicals), 1-13C glucose (Cambridge Isotopes), 1-14C glucose and 6-14C glucose (Perkin Elmer), and Hoechst (Invitrogen).
Salas E., Roy S., Marsh T., Rubin B, & Debnath J. (2015). Oxidative Pentose Phosphate Pathway Inhibition Is A Key Determinant of Antimalarial Induced Cancer Cell Death. Oncogene, 35(22), 2913-2922.
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