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Akt phospho s473 mouse monoclonal antibody

Manufactured by Proteintech
Sourced in United States

The AKT-phospho-S473 mouse monoclonal antibody is a laboratory reagent used to detect the phosphorylation of the serine 473 residue of the AKT protein. This antibody can be utilized in various immunoassay techniques, such as Western blotting, to study the activation and regulation of the AKT signaling pathway.

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2 protocols using akt phospho s473 mouse monoclonal antibody

1

Herceptin Nanoparticle Fabrication

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Herceptin was purchased from Roche Pharma (Schweiz) Ltd. PEG (MW =1000 and 1500) and Sn(Oct)2 were obtained from Sigma-Aldrich. LA and GA were purchased from Hangzhou Medzone Biotech Ltd. (China). Cyanine5.5 N-hydroxysuccinimide ester (Cy5.5-NHS) was obtained from Lumiprobe. An anti-CD69 polyclonal antibody was obtained from Bioss Antibodies Ltd. (USA), and an AKT-phospho-S473 mouse monoclonal antibody was purchased from Proteintech Group, Inc. Other reagents were obtained from Sinopharm Chemical Reagent Co. and directly used.
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2

Antibodies and Primers for Protein Analysis

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The primary antibodies were purchased from Proteintech Group Inc. (IL, USA), including CD31 rabbit polyclonal antibody (#11265-1-AP), VEGF rabbit polyclonal antibody (#19003-1-AP), Akt rabbit polyclonal antibody (#10176-2-AP), Akt-phospho-S473 mouse monoclonal antibody (#66444-1-Ig), HIF-1α rabbit polyclonal antibody (#20960-1-AP), and GAPDH mouse monoclonal antibody (#60004-1-Ig). The primary antibody against canstatin (#DF3550, rabbit polyclonal antibody) was obtained from Affinity Biosciences Inc. (OH, USA). The primary antibody against survivin rabbit polyclonal antibody (#GB11177) was purchased from Servicebio Inc. (Wuhan, China). The HRP-linked goat anti-mouse IgG secondary antibody (#SA00001-1) was purchased from Proteintech Group Inc. (IL, USA). The HRP goat anti-rabbit IgG secondary antibody (#7074) was purchased from Cell Signaling Technology Co. (Danvers, MA, USA). The primers used in this study were synthesized by Takara Biotechnology Co., Ltd (Dalian, China).
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