Glucose
Manufactured by Randox
Sourced in Sweden
Glucose is a laboratory equipment used to measure the concentration of glucose in a sample. It is a crucial tool for monitoring and managing various health conditions, including diabetes.
Automatically generated - may contain errors
6 protocols using glucose
1
Serum Metabolite Quantification
2
Metabolic Biomarkers in Skeletal Muscle
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Blood and gastrocnemius muscle were collected in non-fasting animals the day after the 8-week MR investigation. In order to exclude any bias related to physiological and behavioral circadian oscillations between animals, all sacrifices were performed the same day and within a 60-min time frame starting six hours after light. Transcardiac blood samples (0.25 ml) were obtained during an anesthetic epoch. Plasma was immediately collected after blood centrifugation (15 min at 4,000 rpm) in EDTA-treated tubes. Animals were euthanized by cervical dislocation following isoflurane anesthesia and gastrocnemius muscles were quickly removed, freeze-clamped with liquid nitrogen-chilled metal tongs and stored at -80°C.
Plasmatic concentrations of insulin, glucose and non-esterified fatty acids (NEFA) were measured using insulin (Mercodia, Uppsala, Sweden), glucose (Randox Laboratories, Crumlin, Antrim, UK) and NEFA (Roche Diagnostics, Roche Applied Science, Mannheim, Germany) determination kits.
ATP content was determined in 40-60 mg of freeze-clamped muscle homogenized in 1.2 mL of ice-cold 0.6 M perchloric acid using a Polytron PT2100 (Kinematica AG, Luzern, Switzerland). After incubation (15 min at 4°C), the homogenate was centrifuged (15 min, 2000 x g, 4°C), and the supernatant was neutralized with K 2 CO 3 , placed for 30 min at 4°C and centrifuged (
Plasmatic concentrations of insulin, glucose and non-esterified fatty acids (NEFA) were measured using insulin (Mercodia, Uppsala, Sweden), glucose (Randox Laboratories, Crumlin, Antrim, UK) and NEFA (Roche Diagnostics, Roche Applied Science, Mannheim, Germany) determination kits.
ATP content was determined in 40-60 mg of freeze-clamped muscle homogenized in 1.2 mL of ice-cold 0.6 M perchloric acid using a Polytron PT2100 (Kinematica AG, Luzern, Switzerland). After incubation (15 min at 4°C), the homogenate was centrifuged (15 min, 2000 x g, 4°C), and the supernatant was neutralized with K 2 CO 3 , placed for 30 min at 4°C and centrifuged (
3
Periparturient Metabolic Profiling in Cows
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Blood samples were obtained from the coccygeal vein of all cows on days -14, -7, 0, 7, 14, and 21 relatives to calving using evacuated glass tubes containing EDTA (10.5 mg, Monoject; Sherwood Medical, St. Louis, MO, USA(. The collected blood samples were kept at 4°C, and within 1h after sampling centrifuged at 3000×g for 10 min. The harvested plasma was stored at -8°C for follow-up examinations. Plasma concentration of glucose (ParsAzmoon Co., Tehran, Iran), non-esteri ed fatty acids (NEFA) and beta-hydroxybutyrate (Randox Laboratories Ltd., London, UK) were assessed using commercial kits according to the manufacture's procedures. The NEFA and BHBA inter-assay coe cients were 6.1 and 4.4%, while their intra-assay coe cients were 4.3 and 3.9%, respectively. Blood concentrations of insulin (Diaplus Inc., USA), growth hormone (Monobind Inc Lake Forest, CA, USA), and IGF-1 (Hangzhou Eastbiopharm Co., Ltd., USA) were measured using ELISA kits according to the manufacturer's instructions. Inter-assay and intra-assay coe cients of variation for insulin assay were 7.5 and 5.4%.
4
Peripartum Metabolic and Hormonal Profiles
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Blood samples were collected at 13:00 h from the coccygeal vein of all cows on days –14, –7, 0, 7, 14 and 21 relative to calving using evacuated glass tubes containing EDTA (10.5 mg, Monoject; Sherwood Medical, St. Louis, MO, USA). The collected blood samples were kept at 4°C, within 1 h after sampling, centrifuged at 3000×g for 10 min, and the harvested plasma was stored at −20°C until further evaluation. Plasma concentration of glucose (ParsAzmoon Co., Tehran, Iran), NEFA and beta‐hydroxybutyrate (Randox Laboratories Ltd., London, UK) were assessed using commercial kits according to the manufacturer's procedures. The NEFA and beta‐hydroxybutyrate (BHBA) inter‐assay coefficients were 6.1% and 4.4%, whereas their intra‐assay coefficients were 4.3% and 3.9%, respectively. Blood concentrations of insulin (Diaplus Inc., USA), GH (Monobind, Inc., Lake Forest, CA, USA) and IGF‐1 (Hangzhou Eastbiopharm Co., Ltd., USA) were measured using their corresponding ELISA kits as described in the manufacturer's instructions. Inter‐assay and intra‐assay coefficients of variation were 7.5% and 5.6% for insulin, 5.1% and 3.2% for GH, and 6.8% and 5.4% for IGF‐1 assays.
5
Blood Biomarker Measurement Protocol
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Fasting blood samples were collected to measure plasma triglyceride (TG), total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), glucose (Randox Laboratories. Ltd., U.K.), creatinine, alanine transaminase (ALT), and aspartate aminotransferase (AST) (Fortress, Antrim, U.K.) levels. In addition, the treated cells and liver tissue lysates were collected to measure TG and TC.
6
Comprehensive Assessment of Metabolic Biomarkers
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