For colony formation assays, 1000 cells were seeded in the 6-well plates and incubated with normal growth medium for 14 days. Then cells were fixed and stained with 0.5% crystal violet for 15 min. Lastly, the colonies were imaged via a camera or a microscope. For the anchorage-independent colony formation assay, the cells were collected, resuspended in culturing media containing 10% FBS and 0.3% agarose (Amresco), and then seeded on top of 0.6% agarose gel containing 10% FBS in six-well plates. The cells were cultured in regular media for 3–4 weeks, and the colonies were stained with crystal violet and photographed.
Agarose
Agarose is a polysaccharide derived from seaweed that is commonly used as a gelling agent in various laboratory applications. It is a versatile material that forms a porous, semi-solid matrix when dissolved in water and allowed to cool. Agarose is often used in gel electrophoresis techniques to separate and analyze macromolecules such as DNA, RNA, and proteins based on their size and charge.
Lab products found in correlation
36 protocols using agarose
Cell Proliferation and Colony Formation Assays
For colony formation assays, 1000 cells were seeded in the 6-well plates and incubated with normal growth medium for 14 days. Then cells were fixed and stained with 0.5% crystal violet for 15 min. Lastly, the colonies were imaged via a camera or a microscope. For the anchorage-independent colony formation assay, the cells were collected, resuspended in culturing media containing 10% FBS and 0.3% agarose (Amresco), and then seeded on top of 0.6% agarose gel containing 10% FBS in six-well plates. The cells were cultured in regular media for 3–4 weeks, and the colonies were stained with crystal violet and photographed.
Soft Agar Colony Formation Assay
Soft Agar Colony Formation Assay
Virus Plaque Assay in MDCK Cells
Viral Plaque Assay in MDCK Cells
Cell Colony Formation Assay
Soft Agar Colony Formation Assay
Soft Agar Colony Formation Assay
Reagents and Equipment for Nucleic Acid Analysis
cDNA Synthesis and Gel Electrophoresis
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