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Dionex ase 350 accelerated solvent extractor

Manufactured by Thermo Fisher Scientific
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The Dionex™ ASE 350 Accelerated Solvent Extractor is a lab equipment designed for automated extraction of organic compounds from solid and semisolid samples. It uses elevated temperature and pressure to enhance the extraction process, providing efficient and reproducible results.

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Lab products found in correlation

Savant sc250exp speedvac concentrator, by Thermo Fisher Scientific (2 mentions) Dionex 350 150 extraction cell filters, by Thermo Fisher Scientific (2 mentions) Savant speedvac concentrator sc250 exp, by Thermo Fisher Scientific (1 mentions) Whatman no 40 filter paper, by Cytiva (1 mentions) Rapidvap vacuum evaporation system, by Labconco (1 mentions) Fp628 nitrogen analyzer, by Leco (1 mentions)

20 protocols using dionex ase 350 accelerated solvent extractor

1

Cranberry Fruit and Leaf Extraction Protocol

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Example 1

Dried cranberry fruit powder (Vaccinium macrocarpon) (60 g) was loaded into three 100 ml stainless steel tubes and extracted twice using a solvent of 70% ethanol in DI water with a Thermo Scientific™ Dionex™ ASE 350 Accelerated Solvent Extractor at a temperature of 80° C. and pressure of 1500 psi. The extract solution was automatically filtered and collected. The combined ethanol extract solution was evaporated with a rotary evaporator under vacuum to give a crude 70% ethanol fruit extract (‘E1’).

Dried ground cranberry leaf powder (Vaccinium macrocarpon) (140 g) was loaded into seven 100 ml stainless steel tubes and extracted twice using a solvent of 70% ethanol in DI water with a Thermo Scientific™ Dionex™ ASE 350 Accelerated Solvent Extractor at a temperature of 80° C. and pressure of 1500 psi. The extract solution was automatically filtered and collected. The combined ethanol extract solution was evaporated with a rotary evaporator under vacuum to give a crude 70% ethanol leaf extract (‘E2’).

The extraction results are provided in the following Table 1—

TABLE 1
Extraction of Cranberry fruit and Cranberry leaf
PlantExtraction
Plant PartExtract IDPowder (g)Extract Weight (g)Yield (wt %)
FruitE16027.4045.67%
LeafE214023.7516.96%

US11147849B2. Botanical modulator of metabolic disorders (2021-10-19). Innophos, Inc. [US]. Inventors: Jatinder Rana [US], Kylie Mitchell [US].
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2

Cranberry Extract Yield Optimization

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Example 1

Dried Cranberry fruit powder (Vaccinium macrocarpon) (60 g) was loaded into three 100 ml stainless steel tubes and extracted twice using a solvent of 70% ethanol in DI water with a Thermo Scientific™ Dionex™ ASE 350 Accelerated Solvent Extractor at a temperature of 80° C. and pressure of 1500 psi. The extract solution was automatically filtered and collected. The combined ethanol extract solution was evaporated with a rotary evaporator under vacuum to give a crude 70% ethanol fruit extract (E1).

Dried ground Cranberry leaf powder (Vaccinium macrocarpon) (140 g) was loaded into seven 100 ml stainless steel tubes and extracted twice using a solvent of 70% ethanol in DI water with a Thermo Scientific™ Dionex™ ASE 350 Accelerated Solvent Extractor at a temperature of 80° C. and pressure of 1500 psi. The extract solution was automatically filtered and collected. The combined ethanol extract solution was evaporated with a rotary evaporator under vacuum to give a crude 70% ethanol leaf extract (E2).

The extraction results are provided in the following Table 1—

TABLE 1
Extraction of Cranberry fruit and Cranberry leaf
PlantExtractPlantExtractExtraction
PartIDPowder (g)Weight (g)Yield (wt %)
FruitE16027.4045.67%
LeafE214023.7516.96%

US11141445B2. Botanical antioxidants (2021-10-12). Innophos, Inc. [US]. Inventors: Jatinder Rana [US], Kylie Mitchell [US].
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3

Accelerated Solvent Extraction of Cranberry Fruit and Leaf

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Example 1

Dried cranberry fruit powder (Vaccinium macrocarpon) (60 g) was loaded into three 100 ml stainless steel tubes and extracted twice using a solvent of 70% ethanol in DI water with a Thermo Scientific™ Dionex™ ASE 350 Accelerated Solvent Extractor at a temperature of 80° C. and pressure of 1500 psi. The extract solution was automatically filtered and collected. The combined ethanol extract solution was evaporated with a rotary evaporator under vacuum to give a crude 70% ethanol fruit extract (‘E1’).

Dried ground cranberry leaf powder (Vaccinium macrocarpon) (140 g) was loaded into seven 100 ml stainless steel tubes and extracted twice using a solvent of 70% ethanol in DI water with a Thermo Scientific™ Dionex™ ASE 350 Accelerated Solvent Extractor at a temperature of 80° C. and pressure of 1500 psi. The extract solution was automatically filtered and collected. The combined ethanol extract solution was evaporated with a rotary evaporator under vacuum to give a crude 70% ethanol leaf extract (‘E2’).

The extraction results are provided in the following Table 1—

TABLE 1
Extraction of Cranberry fruit E1 and Cranberry leaf E2
Plant Extract Plant Extract Extraction
PartIDPowder (g)Weight (g)Yield (wt %)
FruitE16027.4045.67%
LeafE214023.7516.96%

US11141446B2. Anti-inflammatory botanical extract (2021-10-12). Innophos, Inc. [US]. Inventors: Jatinder Rana [US], Kylie Mitchell [US].
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4

Phytochemical Extraction using Accelerated Solvent

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Recovery of phytochemicals was carried out using a Dionex ASE 350 Accelerated Solvent Extractor (Dionex Corp., Sunnyvale, CA, USA). Extractions were done with different experimental combinations among solvent composition (ethanol and water), temperatures and extraction time. All extractions were carried out at constant pressure (11 MPa) and under a N2 atmosphere.
To carry out the extractions, the solvents were previously degassed for 15 min to remove the dissolved oxygen in order to avoid any possible oxidation. For each extraction, 3 g of sample were mixed with 9 g of sand and loaded onto 33 mL stainless-steel extraction cells. The chosen configuration was sandwich type (5 g sand + mixture sample − sand + 5 g sand). Cellulose filters were placed at each end of the cell in order to prevent clogging of the metal frits. The extraction conditions described above were applied and the obtained extracts were collected in glass vials. These extracts were quickly cooled to room temperature, filtered, and vacuum evaporated using a Savant SpeedVac Concentrator SC250EXP (Thermo Scientific, Sunnyvale, CA, USA) and stored at −20 °C until HPLC analysis.
Fuentes J.A., López-Salas L., Borrás-Linares I., Navarro-Alarcón M., Segura-Carretero A, & Lozano-Sánchez J. (2021). Development of an Innovative Pressurized Liquid Extraction Procedure by Response Surface Methodology to Recover Bioactive Compounds from Carao Tree Seeds. Foods, 10(2), 398.
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5

Accelerated Solvent Extraction of Cherry Stem Bioactives

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The extraction was carried out using a Dionex ASE 350 Accelerated Solvent Extractor (Dionex Corp., Sunnyvale, CA, USA) equipped with solvent reservoirs, a pump, an oven, a cell tray, and a collection vessel tray, as described previously [19 (link)]. Briefly, 6 g of cherry stem powder was mixed with 12 g of sand and packed into a 34 mL stainless steel extraction cell. Moreover, in order to avoid possible blockage of the system by solid particles, cellulose filters and stainless steel frits were disposed at both sides of the extraction cell. The extraction was carried out with ethanol/water (1:1, v/v), at a temperature of 40 °C. The extraction was performed in static mode for 20 min at 1500 psi. The extract was collected in vials, filtered through 0.2 μm polytetrafluoroethylene (PTFE) syringe filters, and concentrated under vacuum at room temperature using a Savant SC250EXP SpeedVac Concentrator (Thermo Scientific, Waltham, MA, USA). The extracts were stored at −20 °C and protected from light exposure until use.
Agulló-Chazarra L., Borrás-Linares I., Lozano-Sánchez J., Segura-Carretero A., Micol V., Herranz-López M, & Barrajón-Catalán E. (2020). Sweet Cherry Byproducts Processed by Green Extraction Techniques as a Source of Bioactive Compounds with Antiaging Properties. Antioxidants, 9(5), 418.
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6

Accelerated Solvent Extraction of Comfrey Root

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The PLE was carried out using a Dionex ASE 350 Accelerated Solvent Extractor (Dionex Corp., Sunnyvale, CA, USA). The cells were equipped with a stainless-steel frit and a cellulose filter at the bottom to avoid passage of suspended particles into the collection vial. The PLE experiments were performed in a static mode at 1500 psi for 20 min with different ethanol:water mixtures from 0% to 100%) and temperatures (from 40 to 200 °C) to cover a wide range of dielectric constant. Dried comfrey root (6 g) was mixed with 12 g of sand and loaded into a 34 mL stainless steel extraction cell. The extraction conditions described above were applied and the extracts were collected in vials. The residual solvent was evaporated using Savant SC250EXP SpeedVac Concentrator (Thermo Scientific, Sunnyvale, CA, USA) and dried extracts were stored at −20 °C protected from light until analysis.
Nastić N., Borrás-Linares I., Lozano-Sánchez J., Švarc-Gajić J, & Segura-Carretero A. (2020). Comparative Assessment of Phytochemical Profiles of Comfrey (Symphytum officinale L.) Root Extracts Obtained by Different Extraction Techniques. Molecules, 25(4), 837.
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7

Extraction of Cashew Testa Bioactive Compounds

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Example 1

Dried cashew testa powder (Anacardium occidentale) (60 g) was loaded into three 100 ml stainless steel tubes and extracted twice using a solvent of 70% ethanol in DI water with a Thermo Scientific™ Dionex™ ASE 350 Accelerated Solvent Extractor at a temperature of 80° C. and pressure of 1500 psi. The extract solution was filtered and collected. The combined ethanol extract solution was evaporated with a rotary evaporator under vacuum to give a crude cashew testa extract.

The extraction results are provided in the following Table 1—

TABLE 1
Extraction of cashew testa
Extraction Yield
Plant PartPlant Powder (g)Extract Weight (g)(wt %)
Testa6023.7839.63%

US11077160B2. Botanical antioxidants (2021-08-03). Innophos, Inc. [US]. Inventors: Jatinder Rana [US], Kylie Mitchell [US].
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8

Polycyclic Aromatic Hydrocarbons Analysis in Soil

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Chemical analyses were performed in the Chromatography Collective Use Center of the Institute of Biology, Federal Research Center and Komi Research Center of the Ural Branch of the Russian Academy of Sciences. The extraction of PAHs from the soils was made on a Dionex™ ASE™ 350 Accelerated Solvent Extractor (Thermo Fischer Scientific™, Waltham, MA, USA). The extraction was performed three times with a mixture of methylene chloride and acetone (1:1) mixture at 100 °C. The content of polycyclic aromatic hydrocarbons in the concentrates was determined on the basis of US EPA method 8310 (1996a) and certified national standard method of quantitative chemical analysis (PND F 16.1:2.2:2.3:3.62-09. 2009). The relative errors of the determination (p = 0.95, ± δ, %) depended on the measurement range and varied within 16–50 for naphthalene (NP), 20–40 for acenaphthene (ACE), 18–40 for fluorene (FL), 20–50 for phenanthrene (PHE), 18–50 for anthracene (ANT), 18–46 for fluoranthene (FLA) and pyrene (PYR), 20–42 for benzo[a]anthracene (BaA), 22–52 for chrysene (CHR), 22–42 for benzo[b]fluoranthene (BbF), 18–48 for benzo[k]fluoranthene (BkF), 18–50 for benzo[a]pyrene (BaP), 20–48 for dibenzo[a,h]anthracene (DahA), and 22–44 for benzo[g,h,i]perylene (BghiP) and indeno[1,2,3-c,d]pyrene (IcdP).
Startsev V., Gorbach N., Mazur A., Prokushkin A., Karpenko L, & Dymov A. (2022). Macrocharcoal Signals in Histosols Reveal Wildfire History of Vast Western Siberian Forest-Peatland Complexes. Plants, 11(24), 3478.
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9

Switchgrass Solvent Extraction and AFEX Pretreatment

Cited 1 time
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Untreated and AFEX-pretreated switchgrass were solvent extracted using a Dionex ASE 350 Accelerated Solvent Extractor (Thermo Scientific). Samples were placed in 100 mL stainless steel extraction cells and extracted at 1600 psi and 100 °C, 70 °C, and 77 °C for water, ethanol, and ethyl acetate, respectively [12 (link), 19 (link), 64 (link)]. The cells were heated for about 5 min to reach the target extraction temperature, followed by 7 min of static time to achieve maximum extraction. Three extraction cycles of 100 mL rinse volumes were used to extract compounds selectively using a single extraction cell. The extracted biomass was washed thrice, using 100 mL of room temperature distilled autoclaved water in each wash, to remove any residual solvent from the extraction process [31 (link)]. Washed biomass was dried in a custom-designed laminar airflow drying box for 3–6 days until the moisture content was less than 11%. Dried biomass was bagged in airtight Ziploc bags. AFEX pretreatment was then performed, as described previously, on solvent-extracted untreated biomass before enzymatic hydrolysis.
Chipkar S., Smith K., Whelan E.M., Debrauske D.J., Jen A., Overmyer K.A., Senyk A., Hooker-Moericke L., Gallmeyer M., Coon J.J., Jones A.D., Sato T.K, & Ong R.G. (2022). Water-soluble saponins accumulate in drought-stressed switchgrass and may inhibit yeast growth during bioethanol production. Biotechnology for Biofuels and Bioproducts, 15, 116.
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10

Cashew Testa Extraction and Yield Analysis

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Example 1

Dried cashew testa powder (Anacardium occidentale) (60 g) was loaded into three 100 ml stainless steel tubes and extracted twice using a solvent of 70% ethanol in DI water with a Thermo Scientific™ Dionex™ ASE 350 Accelerated Solvent Extractor at a temperature of 80° C. and pressure of 1500 psi. The extract solution was filtered and collected. The combined ethanol extract solution was evaporated with a rotary evaporator under vacuum to give a crude cashew testa extract.

The extraction results are provided in the following Table 1—

TABLE 1
Extraction of cashew testa
Extraction
Plant PartPlant Powder (g)Extract Weight (g)Yield (wt %)
Testa6023.7839.63%

US11590192B2. Botanical antioxidants (2023-02-28). Innophos, LLC [US]. Inventors: Jatinder Rana [US], Kylie Mitchell [US].
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