Cellstar 96-well culture microtiter plates, 10 and 50 mL tubes were from Greiner (Alphen a/d Rijn, the Netherlands). Sheath fluid (FM3D) and drive fluid (MAGPIX) were both purchased from Luminex (Austin, USA). The following mycotoxins and metabolites were purchased from Biopure (Tulln, Austria): FB1, FB2, FB3, OTA, OTB, AFB1, aflatoxin B2 (AFB2), aflatoxin G1 (AFG1), aflatoxin G2 (AFG2), aflatoxin M1 (AFM1), T-2, HT-2, DON, D3G, 3-acetyl-DON (3ADON), 15-acetyl-DON (15ADON), NIV, ZEN, α-zearalenol (α-ZEL) and β-zearalenol (β-ZEL). Zearalenone-14-β-D-glucopyranoside (Z14G), α-zearalenol-14-β-D-glucopyranoside (α-ZELG), β-zearalenol-14-β-D-glucopyranoside (β-ZELG) and zearalenone 14-sulfate (Z14S) were produced according to [52 ] or isolated from Fusarium inoculated rice. Syringeless filter devices (Mini-UniPrep, PTFE) for sample clean-up were purchased from GE Healthcare (Rotterdam, the Netherlands). Acetonitrile (ACN) and methanol (MeOH) were purchased from Biosolve (Valkenswaard, the Netherlands), formic acid (FA) from Merck (Amsterdam, the Netherlands) and ammonium formate (AMF) from Fluka Analytical (Steinheim, Germany). All other chemicals were purchased from VWR International (Amsterdam, the Netherlands).
Acetonitrile acn
Manufactured by Biosolve
Sourced in Netherlands, France
Acetonitrile (ACN) is a clear, volatile, and flammable organic solvent. It is commonly used as a mobile phase component in high-performance liquid chromatography (HPLC) and as a solvent in various laboratory applications.
Automatically generated - may contain errors
Lab products found in correlation
Formic acid, by Merck Group (8 mentions)
Methanol meoh, by Biosolve (6 mentions)
Formic acid, by Biosolve (6 mentions)
Sequencing grade trypsin, by Promega (5 mentions)
Ammonium acetate, by Merck Group (4 mentions)
Sodium chloride (nacl), by Merck Group (4 mentions)
Milli q water purification system, by Merck Group (4 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (3 mentions)
Formic acid, by Avantor (3 mentions)
Trifluoroacetic acid tfa, by Merck Group (3 mentions)
Iodoacetamide, by Merck Group (3 mentions)
Sodium hydroxide (naoh), by Merck Group (3 mentions)
Urea, by Merck Group (3 mentions)
Sodium dodecyl sulfate (sds), by Carl Roth (3 mentions)
Calcium chloride cacl2, by Merck Group (3 mentions)
Bicinchoninic acid bca assay kit, by Thermo Fisher Scientific (3 mentions)
Sequencing grade modified trypsin, by Promega (3 mentions)
Methanol, by Biosolve (2 mentions)
Oasis prime hlb plate, by Waters Corporation (2 mentions)
Glu c, by Roche (2 mentions)
38 protocols using acetonitrile acn
1
Quantification of Mycotoxins using MAGPIX
2
Intact Antibody Structural Analysis
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The therapeutic Abs, rituximab (MabThera), obinutuzumab (GAZYVA), and eculizumab (Soliris) were gifts from Genmab (The Netherlands), and trastuzumab (Herceptin) and anti‐Her2/anti‐CD3‐bispecific were supplied in‐house at Genentech, Inc. Excluding the bispecific, all Ab samples were obtained from expired batches. Prior to use, Ab integrity was checked by native MS to ensure against any post‐translational modifications or structure changes (checked by charge state distribution), and the Abs were expected to be of high integrity. All amino acid sequences searched lacked the N‐terminal signal peptides and are provided in Table S1 . Dithiothreitol (DTT), iodoacetamide (IAA), ammonium acetate (AMAC), acetic acid, formic acid (FA), 8 m tris(hydroxymethyl)aminomethane (Tris), methanol (MeOH), and Cathepsins L and D were purchased from Sigma‐Aldrich (St Louis, MO); phosphate buffer was purchased from Lonza Group AG (Basel, CH). Acetonitrile (ACN) was purchased from Biosolve BV (North Brabant, NL) and Fisher Scientific (Hampton, NH).
3
Zearalenone and Metabolites Biotransformation
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ZEN (CAS registry number 17924‐92‐4; ≥99.0%), α‐ZEL (CAS registry number 36455‐72‐8; >98%), β‐ZEL (CAS registry number 71030‐11‐0; >98%), were purchased from Sigma‐Aldrich (Schnelldorf, Germany). Test chemicals were prepared in dimethyl sulfoxide (DMSO; CAS 67‐68‐5) purchased from Merck (Darmstadt, Germany). Pooled rat and human liver S9 fractions were purchased from Corning (Woburn, MA, USA) and pooled rat and human intestinal S9 fractions were purchased from Xenotech (Kansas City, KS, USA). Uridine 5‐diphosphoglucuronide trisodium salt (UDPGA; CAS registry number 63700‐19‐6) was obtained from Carbosynth (Berkshire, UK). Trizma base (Tris, CAS registry number 77‐86‐1) and alamethicin (from Trichoderma viride; CAS 27061‐78‐5) were obtained from Sigma‐Aldrich (Steinheim, Germany). Magnesium chloride hexahydrate (MgCl2∙6H2O; CAS registry number 7791‐18‐6) and formic acid (FA; CAS registry number 64‐18‐6) were obtained from VWR International (Amsterdam, The Netherlands). Phosphate‐buffered saline (PBS, pH 7.4), was obtained from Gibco (Paisley, UK). Ultra performance liquid chromatography/mass spectrometry (UPLC/MS) grade methanol (MeOH; CAS registry number 67‐56‐1) and acetonitrile (ACN; CAS registry number 75‐05‐8) were purchased from Biosolve (Valkenswaard, The Netherlands).
4
Analytical quantification of yohimbine and metabolite
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Yohimbine hydrochloride was kindly provided by Herband Pharmachemicals (Gengenbach, Germany). Pierre Fabre laboratoires (Toulouse, France) kindly donated 11-OH-yohimbine (5.1 mg, 98% purity) and internal standard (IS; [ 13 C 2 H 3 ]-yohimbine) was purchased from Alsachim (Illkirch, France). Yohimbine tablets for oral microdoses (Yohimbinum hydrochloricum D4 R ) were purchased from Deutsche Homöopathie Union (Karlsruhe, Germany). Boric acid and ammonium acetate were obtained from Sigma-Aldrich (Darmstadt, Germany). Acetonitrile (ACN), isopropanol, acetic acid, formic acid (FA), sodium hydroxide, methyl tert-butyl ether (TBME) and methanol were purchased from Biosolve BV (Valkenswaar, The Netherlands) in the highest available purity (ULC/MS grade). For calibration standards, quality controls (QCs) and validation-purpose drug-free plasma from healthy subjects were obtained at the local blood bank.
5
Docetaxel Polymer Conjugate Synthesis
6
Reagent Procurement for Proteomic Analysis
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Unless otherwise specified, all chemicals and reagents were obtained from Sigma-Aldrich (Steinheim, Germany). GluC was obtained from Roche (Indianapolis, IN). The Oasis PRiME HLB plate was purchased from Waters (Etten-Leur, the Netherlands). Formic acid (FA) was purchased from Merck (Darmstadt, Germany). Acetonitrile (ACN) was purchased from Biosolve (Valkenswaard, The Netherlands). Milli-Q was produced by an in-house system (Millipore, Billerica, MA), phosphoSTOP (Roche, Woerden, the Netherlands) and complete mini EDTA free (Roche, Woerden, the Netherlands).
7
Detailed Metabolic Enzyme Assay Protocol
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Daidzein, S-equol, dimethyl
sulfoxide (DMSO), glycerol, alamethicin, uridine 5′-diphosphoglucuronic
acid (UDPGA), 3′-phosphoadenosine-5′-phosphosulfate
(PAPS), and tromethamine (Tris) were obtained from Sigma-Aldrich (Zwijndrecht,
The Netherlands). DHD and O-DMA were purchased from
Cayman Chemical (AA) and Plantech (Reading), respectively. Trifluoroacetic
acid (TFA), MgCl2, 37% HCl and NaOH were obtained from
VWR (Amsterdam, The Netherlands).
Acetonitrile (ACN) and methanol
were obtained from Biosolve BV (Valkenswaard, The Netherlands). Phosphate
buffer saline (PBS) was supplied by Gibco (Paisley). Para-Pak SpinCon
concentration system was bought from Meridian Bioscience (Schijndel,
The Netherlands). Human pooled liver S9 fractions from 25 individuals
(mixed gender) were supplied by Tebu-bio (Heerhugowaard, The Netherlands).
sulfoxide (DMSO), glycerol, alamethicin, uridine 5′-diphosphoglucuronic
acid (UDPGA), 3′-phosphoadenosine-5′-phosphosulfate
(PAPS), and tromethamine (Tris) were obtained from Sigma-Aldrich (Zwijndrecht,
The Netherlands). DHD and O-DMA were purchased from
Cayman Chemical (AA) and Plantech (Reading), respectively. Trifluoroacetic
acid (TFA), MgCl2, 37% HCl and NaOH were obtained from
VWR (Amsterdam, The Netherlands).
Acetonitrile (ACN) and methanol
were obtained from Biosolve BV (Valkenswaard, The Netherlands). Phosphate
buffer saline (PBS) was supplied by Gibco (Paisley). Para-Pak SpinCon
concentration system was bought from Meridian Bioscience (Schijndel,
The Netherlands). Human pooled liver S9 fractions from 25 individuals
(mixed gender) were supplied by Tebu-bio (Heerhugowaard, The Netherlands).
8
Analytical Workflow for Environmental Contaminants
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All standards were obtained from Sigma-Aldrich (Dr. Ehrenstorfer, Augsburg, Germany, purity > 99%). Methanol (MeOH), ammonia (35%), hydrochloric acid (37%), and formic acid (98%) were LCMS grade and purchased from Fischer Scientific (Illkirch Cedex, France). Ethyl acetate (EtAc), dichloromethane (DCM), and acetone were obtained from Merck (Darmstadt, Germany), and acetonitrile (ACN) was purchased from Biosolve (Dieuze, France), at analytical grade. HPLC water (Milli-Q) was produced from deionized water using a Millipore Milli-Q system (IQ 7000, Merck, Darmstadt, Germany) equipped with an LC-pak polisher (Merck, Darmstadt, Germany).
Deuterated standards were used to evaluate matrix effects from different samples, to evaluate the extraction efficiency on the various SPE phases, and to correct the retention time of the chromatograms. A mixed solution of 4 deuterated compounds (atenolol-d7, caffeine-d9, carbamazepine-d8, sulfamethoxazole-d4) was prepared in MeOH at a concentration of 10 mg/L for further injection in samples.
Before sampling and analysis, glassware was washed with TFD4 (Franklab, Montigny-le-Bretonneux, France), rinsed with deionized water, and calcined at 500 °C to remove any trace of organic contamination.
Deuterated standards were used to evaluate matrix effects from different samples, to evaluate the extraction efficiency on the various SPE phases, and to correct the retention time of the chromatograms. A mixed solution of 4 deuterated compounds (atenolol-d7, caffeine-d9, carbamazepine-d8, sulfamethoxazole-d4) was prepared in MeOH at a concentration of 10 mg/L for further injection in samples.
Before sampling and analysis, glassware was washed with TFD4 (Franklab, Montigny-le-Bretonneux, France), rinsed with deionized water, and calcined at 500 °C to remove any trace of organic contamination.
9
Proteomic Sample Preparation Protocol
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Ammonium acetate,
ammonium formate (AF), ethylenediaminetetraacetic acid (EDTA), iodoacetamide
(IAM), sodium deoxycholate (DOC), and tris(hydroxymethyl)aminomethane
(TRIS) were obtained from Sigma-Aldrich (Zwijndrecht, The Netherlands).
Ammonia solution 32%, calcium chloride, hydrochloric acid 37%, and
phosphate-buffered saline (PBS) were purchased from Merck-Millipore
(Burlington, MA), and tris(2-carboxyethyl)phosphine (TCEP) was purchased
from Thermo Scientific (Rockford, IL). Ammonium bicarbonate (ABC)
and formic acid (FA) were obtained from Honeywell (Morristown, NJ).
Milli-Q water (MQ) was generated from a QGard2 system (at ≥18
MΩ) from Merck-Millipore (Burlington, MA). Sequencing-grade
modified porcine trypsin and sequencing-grade ArgC were purchased
from Promega (Madison, WI). Neuramidase A and S were obtained from
New England Biolabs (Ipswich, MA). HPLC-grade solvents methanol (MeOH)
and acetonitrile (ACN) were purchased from Biosolve (Valkenswaard,
The Netherlands). In all experiments a standard PSA sample was used
from Lee Biosolutions (St. Louis, MO) derived from a pool of human
semen. Stable-isotope-labeled (SIL, heavy amino acid is indicated
with an asterisk) and nonlabeled peptide standards were synthesized
in-house, dissolved in 5% MeOH, and stored at −80 °C until
further use.
ammonium formate (AF), ethylenediaminetetraacetic acid (EDTA), iodoacetamide
(IAM), sodium deoxycholate (DOC), and tris(hydroxymethyl)aminomethane
(TRIS) were obtained from Sigma-Aldrich (Zwijndrecht, The Netherlands).
Ammonia solution 32%, calcium chloride, hydrochloric acid 37%, and
phosphate-buffered saline (PBS) were purchased from Merck-Millipore
(Burlington, MA), and tris(2-carboxyethyl)phosphine (TCEP) was purchased
from Thermo Scientific (Rockford, IL). Ammonium bicarbonate (ABC)
and formic acid (FA) were obtained from Honeywell (Morristown, NJ).
Milli-Q water (MQ) was generated from a QGard2 system (at ≥18
MΩ) from Merck-Millipore (Burlington, MA). Sequencing-grade
modified porcine trypsin and sequencing-grade ArgC were purchased
from Promega (Madison, WI). Neuramidase A and S were obtained from
New England Biolabs (Ipswich, MA). HPLC-grade solvents methanol (MeOH)
and acetonitrile (ACN) were purchased from Biosolve (Valkenswaard,
The Netherlands). In all experiments a standard PSA sample was used
from Lee Biosolutions (St. Louis, MO) derived from a pool of human
semen. Stable-isotope-labeled (SIL, heavy amino acid is indicated
with an asterisk) and nonlabeled peptide standards were synthesized
in-house, dissolved in 5% MeOH, and stored at −80 °C until
further use.
10
Analytical Standards for Pharmacological Studies
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Acetonitrile (ACN) and methanol (MeOH) (LC-MS grade) were purchased from Biosolve (Valkenswaard, the Netherlands). Methanol (HPLC grade) and dimethylsulfoxide (DMSO, 99.9%, for spectroscopy) were ordered from Acros Organics (Geel, Belgium). Ethyl acetate (EtOAc, 99.9%) were purchased from VWR (Haasrode, Belgium), while Chem-Lab (Zedelgem, Belgium) supplied hexane (C6H14, >99%, p.a.). Hanks’ Balanced Salt Solution without phenol red (HBSS) was ordered from Lonza (Verviers, Belgium). Merck (Darmstadt, Germany) supplied sodium hydroxide (NaOH, pellets, p.a.) and disodiumtetraborate decahydrate (Na2B4O7.10H2O, borax, 99–103%). Ritonavir (RTV) powder was purchased from Sigma-Aldrich (St. Louis, MO, USA.) for analytical purposes. Lopinavir (LPV) powder was donated by Hetero Drugs Ltd. (Hyderabad, India). Water was purified using a Maxima System (Elga Ltd., High Wycombe Bucks, UK).
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