Log In Sign up
The largest database of trusted experimental protocols

Navious

Manufactured by Beckman Coulter
Visit Supplier
Sourced in United States

The Navious is a high-performance liquid chromatography (HPLC) system designed for efficient separation, identification, and quantification of complex mixtures. It features advanced technology for precise and reliable analysis across a wide range of applications.

Automatically generated - may contain errors

Lab products found in correlation

Opti mem 1, by Thermo Fisher Scientific (1 mentions) Lipofectamine rnaimax, by Thermo Fisher Scientific (1 mentions) Rnase a, by Merck Group (1 mentions) Kaluza software, by Beckman Coulter (1 mentions) Annexin 5 pe detection kit 1, by BD (1 mentions)

Close spelling variants of this product

Navious EX Navious flow cytometer

7 protocols using navious

1

Cell Cycle Analysis by Flow Cytometry

Check if the same lab product or an alternative is used in the 5 most similar protocols
Daoy (2×105/well) and MEB-Med-8A (3×105 / well) cells respectively were plated in 6-well cell culture dishes. After 48h of treatment with the respective MKI the cells were exposed to 16 nM Hoechst 33342 and incubated for 45min at 37°C. Both floating and attached cells were harvested and analyzed by flow cytometry (Navious, Beckman Coulter). Dead cells were stained by Propidium Iodid (PI). After gating on live cells, single cells were gated using width and area parameters from Hoechst 33342. The area parameter histogram was used to determine the percentage of cells in G1, S and G2M phases.
Craveiro R.B., Ehrhardt M., Holst M.I., Pietsch T, & Dilloo D. (2014). In comparative analysis of multi-kinase inhibitors for targeted medulloblastoma therapy pazopanib exhibits promising in vitro and in vivo efficacy. Oncotarget, 5(16), 7149-7161.
+ Open protocol
+ Expand
2

Lipofectamine RNAiMAX Transfection Efficiency

Check if the same lab product or an alternative is used in the 5 most similar protocols
Lipofectamine RNAiMAX (Invitrogen, USA) and four sequences of ITGA5 siRNAs (GeneSolution, Qiagen, Germany) were added to OPTIMEM-I (GIBCO, USA). AllStars Neg. siRNA AF488 served as control. Transfection efficiency was evaluated using flow cytometry (Navious, Beckman Coulter).
Shochet G.E., Brook E., Bardenstein-Wald B., Grobe H., Edelstein E., Israeli-Shani L, & Shitrit D. (2020). Integrin alpha-5 silencing leads to myofibroblastic differentiation in IPF-derived human lung fibroblasts. Therapeutic Advances in Chronic Disease, 11, 2040622320936023.
+ Open protocol
+ Expand
3

Cell Cycle Analysis of 5-FU and CUDC-907 Treatment

Check if the same lab product or an alternative is used in the 5 most similar protocols
HCT116 cells were treated with dimethyl sulfoxide (DMSO) as control, 5-FU, CUDC-907, or combination of 5-FU and CUDC-907. On day 5, cell pellets were collected and washed with phosphate-buffered saline (PBS), and then were resuspended in 1 ml of FACS buffer (PBS/0.5% BSA), and then 3 ml of ice-cold 70% ethanol was added to fix the cells for 1 h on ice. Cell pellets were subsequently centrifuged, and re-suspended in 500 μL of PBS supplemented with 40 µg/mL RNAse A (Sigma) and 50 µg/mL propidium iodine (PI), before being analyzed using a Navious flow cytometer (Beckman Coulter, Miami, FL, USA). Staining was detected in the green fluorescence channel (FL1) and the data were analyzed by Kaluza software (Beckman Coulter).
Hamam R., Ali D., Vishnubalaji R., Alsaaran Z.F., Chalisserry E.P., Alfayez M., Aldahmash A, & Alajez N.M. (2017). Enhanced Efficacy of 5-Fluorouracil in Combination with a Dual Histone Deacetylase and Phosphatidylinositide 3-Kinase Inhibitor (CUDC-907) in Colorectal Cancer Cells. Saudi Journal of Gastroenterology : Official Journal of the Saudi Gastroenterology Association, 23(1), 34-38.
+ Open protocol
+ Expand
4

Medulloblastoma Cell Apoptosis Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
Medulloblastoma cells were stained with CFSE according to the supplier's instructions. Daoy (3×105/well), MEB-Med8A (5×105/well) D283 Med (5×105/well) and D341 Med (5×105/well) cells were seeded in 6-well cell culture dishes in complete medium. After overnight culture, the cells were treated with MKIs at concentrations corresponding to patient plasma levels for a 24h, 48h or 72h period. Thereafter floating and attached cells were collected and stained with 7-AAD and Annexin V-Antibody (Annexin V-PE Detection Kit I, BD Bioscience) and analysed by flow cytometry (Navious, Beckman Coulter). Proliferation was traced by CFSE staining and apoptosis was detected by combined 7AAD/Annexin V staining and calculated in percent of control.
Craveiro R.B., Ehrhardt M., Holst M.I., Pietsch T, & Dilloo D. (2014). In comparative analysis of multi-kinase inhibitors for targeted medulloblastoma therapy pazopanib exhibits promising in vitro and in vivo efficacy. Oncotarget, 5(16), 7149-7161.
+ Open protocol
+ Expand
5

Beta-Galactose Glycan Analysis by FC

Check if the same lab product or an alternative is used in the 5 most similar protocols
Glycan pattern was analyzed by flow cytometer (FC) (Navious, Beckman Coulter) using ricinus communis agglutinin (RCA, 0.5 mg/mL, Vector, Burlingame, CA, USA) which binds beta (β)-galactose, as described in the Online Supplementary Methods.
Marini I., Aurich K., Jouni R., Nowak-Harnau S., Hartwich O., Greinacher A., Thiele T, & Bakchoul T. (2019). Cold storage of platelets in additive solution: the impact of residual plasma in apheresis platelet concentrates. Haematologica, 104(1), 207-214.
+ Open protocol
+ Expand
6

Cell Cycle Analysis of Daoy and MEB-Med-8A Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Daoy (2 × 105/6 well) and MEB-Med-8A (3 × 105/well) cells respectively were plated in 6-well cell culture dishes. After 48 h of treatment with GDC-0941 the cells were exposed to 16 nM Hoechst 33342 and incubated for 45min at 37°C. Both floating and attached cells were harvested and analyzed by flow cytometry (Navious, Beckman Coulter). Dead cells were stained by propidium iodide. After gating on live cells, single cells were gated using width and area parameters from Hoechst 33342. The area parameter histogram was used to determine the percentage of cells in G1, S and G2M phases.
Ehrhardt M., Craveiro R.B., Holst M.I., Pietsch T, & Dilloo D. (2014). The PI3K inhibitor GDC-0941 displays promising in vitro and in vivo efficacy for targeted medulloblastoma therapy. Oncotarget, 6(2), 802-813.
+ Open protocol
+ Expand
7

Cell Cycle and Apoptosis Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Transfected cells were harvested , xed , stained and analyzed by ow cytometry (Navious, Beckman Coulter) for cell cycle pro le determination [21] . Apoptosis was measured by staining with 7AAD/APC. The results were analyzed using Flowjo software (CT, USA).
Li Y., Wu D., Hou J., Zhang J., Zeng X., Chen L., Wan X., Wu Z., Wang J., Wang L., Yang D., Chen J., Xu Z., Jia L., Liu Q., Kuang Z., Jiang G., Zhang H., Luo J., Li W., Zou X., Zhang F., & Zhou D. (2020). Characterization of a Novel MAP4K4-SASH1 Kinase Cascade Regulating Breast Cancer Tumorigenesis and Metastasis.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!