Accu chek active glucose strips

Blood glucose levels were measured every 2 weeks from a drop of blood by tail incision using Accu-Chek blood glucose meter and Accu-Chek Active glucose strips (ROCHE, Switzerland). Whereas, glycated hemoglobin (HbA1c) was assessed using the BIOHRMES HbA1c Analyzer (BIOHERMES, USA) at the 8th week following the first injection, and the 6th week following the second injection. Besides, the intraperitoneal glucose tolerance test (IPGTT) was performed using the Accu-Check blood glucose meter. Briefly, after six hours of fasting, mice body weights and fasting blood glucose levels were recorded (t = 0 min). Mice were then injected with 20% glucose. The volume of the glucose injection was calculated according to the formula: volume = 10 × lean body mass (g)⁴¹ . Blood glucose levels were then measured at 15, 30, 60 and 120 min post-injection.

Experimental DM was induced by intraperitoneal (IP) injection of freshly prepared alloxan dissolved in sterile saline (0.9%) every 48 h for three times at a dose of 180 mg/kg. Fasting glucose levels in the blood samples obtained from the tail of each mouse 72 h after the last alloxan injection were measured with Accu-Chek Active glucose strips in Accu-Chek Active Test Meter (Roche, USA). The glucose levels were expressed as mg/dL. Glucose (5%) was added to mice drinking water. The mice with blood glucose level more than 200 mg/dL were considered to be diabetic and were used in the experiment.