The zeta potential of pseudomonal LPS (10 mg/ml) in the absence and presence of OligoG CF-5/20 (2 mg/ml) was determined using a Zetasizer Nano ZS (Malvern Instruments) with disposable capillary cells (DTS1061 Malvern Instruments) in 0.01 M NaCl, pH 5, 7 and 9, at 25 °C (n = 10).
Dts1061
Manufactured by Malvern Panalytical
Sourced in United Kingdom, Germany
The DTS1061 is a differential thermal analysis (DTA) instrument manufactured by Malvern Panalytical. It is designed to measure the thermal behavior of materials as a function of temperature and time. The DTS1061 can detect phase transitions, chemical reactions, and other thermal events in a sample.
Automatically generated - may contain errors
Lab products found in correlation
Zetasizer nano z, by Malvern Panalytical (14 mentions)
Pcs8501, by Malvern Panalytical (2 mentions)
Polyvinyl alcohol (pva), by Merck Group (1 mentions)
Zetasizer nano series, by Malvern Panalytical (1 mentions)
Spectrostar nano, by BMG Labtech (1 mentions)
Spectrum two, by PerkinElmer (1 mentions)
Nano zs90, by Malvern Panalytical (1 mentions)
Dts1232, by Malvern Panalytical (1 mentions)
Nano z, by Malvern Panalytical (1 mentions)
Zen3600, by Malvern Panalytical (1 mentions)
Zetasizer nano zs instrument, by Malvern Panalytical (1 mentions)
Zeta potential nano zs, by Malvern Panalytical (1 mentions)
22 protocols using dts1061
1
Zeta Potential of Pseudomonal LPS
2
Characterization of Magnetic Nanoparticles
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For all used MNP formulations, the hydrodynamic diameter and the zeta potential were determined using a Zetasizer Nano ZS and DTS1061 disposable capillary cells (both Malvern Instruments GmbH, Germany). The hydrodynamic diameter (z-average) was measured in quintuplicates by dynamic light scattering, and the zeta potential (ζ-potential), representing the particle’s surface charge, was measured in triplicates.
The SAR of the used MNPs was determined as described elsewhere using an AMF (H =15.4 kA/m, f =435 kHz).11 (link),28 For this purpose, temperatures were acquired by a fiber optic temperature sensor and a fiber optic thermometer (TS5 and FOTEMPMK-19, both OPTOCON AG, Germany), and the relevant iron concentrations were determined in triplicates by atomic absorption spectroscopy (AAS). The SAR of immobilized MNPs, as occurring after cellular internalization and/or binding, was measured after their immobilization in polyvinyl alcohol (PVA 10%, w/v, Sigma-Aldrich).11 (link),29 (link)–31 (link) High resolution electron microscopy (HRTEM) pictures of MNPs were obtained as described elswere.11 (link)
The SAR of the used MNPs was determined as described elsewhere using an AMF (H =15.4 kA/m, f =435 kHz).11 (link),28 For this purpose, temperatures were acquired by a fiber optic temperature sensor and a fiber optic thermometer (TS5 and FOTEMPMK-19, both OPTOCON AG, Germany), and the relevant iron concentrations were determined in triplicates by atomic absorption spectroscopy (AAS). The SAR of immobilized MNPs, as occurring after cellular internalization and/or binding, was measured after their immobilization in polyvinyl alcohol (PVA 10%, w/v, Sigma-Aldrich).11 (link),29 (link)–31 (link) High resolution electron microscopy (HRTEM) pictures of MNPs were obtained as described elswere.11 (link)
3
Polyplex Characterization by DLS
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The size of the polyplexes and the surface charge were measured with a dynamic light scattering (DLS) spectroscopy (Zetasizer Nano ZS, Malvern Instruments, MA, USA) using a He-Ni laser with a wavelength of 633 nm at a scattering angle of 173°C. Approximately 1,200 μL of polyplex solution containing 5 μg of DNA was prepared at various weight ratios (1, 5, 10, 20, and 40). The mixtures were vortexed for 60 seconds before measurement. The particle size measurement was performed in triplicate at 25°C. Zeta potential measurements were performed using a capillary zeta potential cell (DTS 1061, Malvern Instruments) in automatic mode.
4
Zeta Potential and Particle Size Analysis
5
Peptide Size Measurement in Solution
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
A 1 mL aliquot of Chp F solution was placed in a disposable capillary cell (DTS1061, Malvern Instruments Ltd., Malvern, UK). The size of the peptide in solution was measured at a fixed scattering angle of 173° at 25 °C using a Nano series zeta-sizer (Malvern Instruments Ltd.). Three replicate measurements were performed for each pH value, and the data presented is the average of these replicates with the standard deviation.
6
Bacterial Zeta Potential Measurements
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Bacteria (1 ml) from mid-exponential-phase cultures were washed twice with deionized water and diluted (107 CFU/ml) in 15 mM NaCl solutions adjusted to different pH values (1 to 7) with nitric acid. Bacterial suspensions (750 μl) were injected into a disposable capillary cell cuvette (DTS1061, Malvern Instruments) and the zeta potential was measured at 37°C in a ZetaSizer Nano ZS (Malvern Instruments), under an automated field voltage. Samples were measured in triplicate in three independent assays.
7
Citrate-Stabilized Gold Nanoparticle Synthesis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Citrate AuNPs were prepared as previously reported [49 ]. In a 250 mL flask, 2.5 mL of a 10 mM tetrachloroauric acid trihydrate (HAuCl4) in 90 mL water was heated to boil with vigorous stirring. Once boiling, 7.5 mL of preheated 1% sodium citrate was added rapidly. This solution was left to boil for an additional 10 min, at which point it was removed from heat and allowed to cool to room temperature while stirring. The size of the nanoparticles was determined from analysis of the dynamic light scattering (DLS) (Malvern Zetasizer Nano ZS). Zeta potential measurements were done using a clear zeta disposable capillary (Malvern DTS1061). The AuNPs were concentrated by centrifuge at 10 °C for 20 min before each use and the concentration was measured by SPECTROStarNano (BMG Labtech Inc.).
8
Zeta Potential Measurements of Nanoparticles
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Nanoparticle suspensions were transferred to zetasizer cuvettes (DTS1061, Malvern) and flushed with filtered deionised water. Zeta potential measurements were made in triplicate for nanoparticle suspensions in deionised water and PBS (0.001 M) (parameters used for dispersant deionised water dispersant: refractive index, 1.330; viscosity, 0.8872 cP; dielectric constant, 78.5 εr; Model Smoluchowski F (Ka), 1.5). All samples were equilibrated to 25 °C for 120 s prior to measurement. All measurements were conducted in triplicate.
9
Zeta Potential Analysis of Dried Powders
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
ζ-Potential
distributions of dried powders suspended in water were measured by
dynamic light scattering (DLS) with a Zetasizer Nano ZS (Malvern Ltd,
Worcestershire, U.K.) and were quantified by laser Doppler velocimetry
as electrophoretic mobility using a disposable electrophoretic cell
(DTS1061, Malvern Ltd., Worcestershire, U.K.). A total of 10 runs
of 30 s were performed for each measurement, and four measurements
were carried out for each sample. Zeta average values were obtained
by suspending the dry powders in the same media at a concentration
of 1.0 mg/mL. A total of 20 runs of 30 s each were performed for each
measurement and for each sample.
distributions of dried powders suspended in water were measured by
dynamic light scattering (DLS) with a Zetasizer Nano ZS (Malvern Ltd,
Worcestershire, U.K.) and were quantified by laser Doppler velocimetry
as electrophoretic mobility using a disposable electrophoretic cell
(DTS1061, Malvern Ltd., Worcestershire, U.K.). A total of 10 runs
of 30 s were performed for each measurement, and four measurements
were carried out for each sample. Zeta average values were obtained
by suspending the dry powders in the same media at a concentration
of 1.0 mg/mL. A total of 20 runs of 30 s each were performed for each
measurement and for each sample.
10
Characterization of MNP Formulations
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The hydrodynamic diameter of the used MNP formulations was measured in quintuplicates at a concentration of 50 μg/mL in bidistilled water by dynamic light scattering using a Zetasizer Nano ZS and DTS1061 disposable capillary cells (both Malvern Instruments GmbH, Herrenberg, Germany) with a measurement angle of 173° backscatter. Within this publication, the hydrodynamic diameter refers to the z-average, the most proper value for size determination provided by dynamic light scattering for small polydispersity indices (<0.3). Coincidently, the ζ-potential, which represents the particle’s surface charge, was measured in triplicates. The standard error of the mean was calculated by using the mean values of at least five independent experiments. Used particles showed a sufficient heating capability (specific absorption rate) for later in vivo applications as determined in earlier experiments.10 (link)
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!