Modular preanalytics system

Manufactured by Roche

Sourced in Switzerland

The Modular Preanalytics System is a laboratory equipment designed to automate the preanalytical processes in clinical laboratories. It streamlines the handling and preparation of samples prior to analysis, ensuring consistent and reliable results. The system is modular, allowing for customization to meet the specific needs of individual laboratories.

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Lab products found in correlation

F4 80, by Abcam (1 mentions) Anti mouse f4 80, by Bio-Rad (1 mentions)

8 protocols using modular preanalytics system

Serum ALT and AST Quantification

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Alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activities were measured in serum from mice according to standard methods (UV test at 37 °C) using a Roche Modular preanalytics system (Roche, Grenzach, Germany).

Sonntag R., Gassler N., Bangen J.M., Trautwein C, & Liedtke C. (2014). Pro-apoptotic Sorafenib signaling in murine hepatocytes depends on malignancy and is associated with PUMA expression in vitro and in vivo. Cell Death & Disease, 5(1), e1030-.

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Histological and Biochemical Evaluation of NAFLD

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Conventional hematoxylin-eosin (H&E) and sirius red stainings were performed according to established protocols and necrotic area fraction was quantified with ImageJ [16 (link)]. NAFLD activity score (NAS) was assessed by a medically qualified investigator blinded to the treatment groups, and colometric tests were conducted for hydroxyproline. Immunohistochemistry stainings for F4/80 (Abcam) were performed on paraffin-embedded liver sections [51 (link)]. Alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activities were measured (UV test at 37 °C) in serum (Roche Modular pre-analytics system, Rotkreuz, Switzerland).

Puengel T., Lefere S., Hundertmark J., Kohlhepp M., Penners C., Van de Velde F., Lapauw B., Hoorens A., Devisscher L., Geerts A., Boehm S., Zhao Q., Krupinski J., Charles E.D., Zinker B, & Tacke F. (2022). Combined Therapy with a CCR2/CCR5 Antagonist and FGF21 Analogue Synergizes in Ameliorating Steatohepatitis and Fibrosis. International Journal of Molecular Sciences, 23(12), 6696.

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Liver Histological Analysis Protocols

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Alanine (ALT) activities (UV test at 37°C) were measured from serum (Roche Modular preanalytics system, Rotkreuz Switzerland). Conventional H&E, Sirius red, and Ladewig staining were performed according to standard protocols [20] (link). Steatosis was scored by an experienced pathologist blinded to experimental data, as previously described [9] (link), [15] (link). Liver sections from fixed paraffin blocks were immunohistochemically stained according to standard procedures using anti-mouse F4/80 and anti-human CD68 (Serotec), respectively [21] (link). CD68 and H&E pictures were analysed by quantifying the area fraction using imaging software in a blinded fashion (ImageJ).

Wehr A., Baeck C., Ulmer F., Gassler N., Hittatiya K., Luedde T., Neumann U.P., Trautwein C, & Tacke F. (2014). Pharmacological Inhibition of the Chemokine CXCL16 Diminishes Liver Macrophage Infiltration and Steatohepatitis in Chronic Hepatic Injury. PLoS ONE, 9(11), e112327.

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Quantifying Necrotic Areas via H&E Analysis

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Conventional hematoxylin-eosin (H&E) was performed according to established protocols, and necrotic areas were quantified by image analysis [7 (link)]. Alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activities were measured (UV test at 37°C) in serum (Roche Modular pre-analytics system, Rotkreuz, Switzerland).

Puengel T., Krenkel O., Kohlhepp M., Lefebvre E., Luedde T., Trautwein C, & Tacke F. (2017). Differential impact of the dual CCR2/CCR5 inhibitor cenicriviroc on migration of monocyte and lymphocyte subsets in acute liver injury. PLoS ONE, 12(9), e0184694.

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Liver Biochemical and Histological Analyses

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Aspartate aminotransferase and ALT enzyme activities were measured (UV test at 37°C) in serum (Roche Modular pre-analytics system; Roche Diagnostics, Rotkreuz, Switzerland). The hepatic hydroxyproline content (reflecting total intrahepatic collagen) was measured as described before.⁸ (link) Conventional hematoxylin and eosin, Oil red O, and Sirius Red staining were performed according to established protocols.⁴⁴ (link) Liver sections from paraffin were immunohistochemically stained for CD45, F4/80, or αSMA, as published previously.²⁷ (link)

Bartneck M., Koppe C., Fech V., Warzecha K.T., Kohlhepp M., Huss S., Weiskirchen R., Trautwein C., Luedde T, & Tacke F. (2020). Roles of CCR2 and CCR5 for Hepatic Macrophage Polarization in Mice With Liver Parenchymal Cell-Specific NEMO Deletion. Cellular and Molecular Gastroenterology and Hepatology, 11(2), 327-347.

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Acute acetaminophen-induced liver injury

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C57BL/6J mice were housed under specific pathogen free conditions (Janvier labs, Le Genedt-Saint-Isle, France) and were divided into 3 groups (each n = 8): (1) acute liver injury sacrified at 12 h (APAP12), (2) acute liver injury sacrified at 24 h (APAP24), (3) an untreated control group (CTRL). Acute liver injury was induced as described in detail before^{10 (link),11 (link)}. Briefly, after a fasting period of 12 h, the animals received 250 mg/kg of APAP (Actavis Deutschland GmBh& CO. Kg, Langenfeld, Germany) by a single i.v. injection into a tail vein. After sacrifice, diaphragm was removed quickly and further processed (see below). Alanine aminotransferase (ALT) and aspartate aminotransferase activity (UV test at 37 °C) was measured in serum (Roche modular preanalytics system; Roche Diagnostics International AG, Rotkreuz, Switzerland).

Bruells C.S., Duschner P., Marx G., Gayan-Ramirez G., Frank N., Breuer T., Krenkel O., Tacke F, & Mossanen J.C. (2021). Acute liver injury following acetaminophen administration does not activate atrophic pathways in the mouse diaphragm. Scientific Reports, 11, 6302.

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Serum Enzyme Activity Measurement

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Alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activities were measured in murine serum by standard methods (UV test at 37 °C, central diagnostic laboratory Aachen University Hospital) using an automated Roche Modular preanalytics system (Hoffmann-La Roche).

Sonntag R., Penners C., Kohlhepp M., Haas U., Lambertz D., Kroh A., Cramer T., Ticconi F., Costa I.G., Tacke F., Gassler N., Trautwein C, & Liedtke C. (2021). Cyclin E1 in Murine and Human Liver Cancer: A Promising Target for Therapeutic Intervention during Tumour Progression. Cancers, 13(22), 5680.

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APAP-Induced Acute Liver Injury Model

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Acute liver injury was induced as described before.7 (link) Mice were fasted for 12 hours before receiving 250 mg/kg of APAP (Actavis Deutschland GmbH & Co. KG, Langenfeld, Germany) by a single i.v. injection. Mice were euthanized after 3, 6, and 12 hours. Alanine aminotransferase activity was measured in blood serum (Roche Modular preanalytics system; Roche Diagnostics International AG, Rotkreuz, Switzerland).

Heymann F., Mossanen J.C., Peiseler M., Niemietz P.M., Araujo David B., Krenkel O., Liepelt A., Batista Carneiro M., Kohlhepp M.S., Kubes P, & Tacke F. (2023). Hepatic C-X-C chemokine receptor type 6–expressing innate lymphocytes limit detrimental myeloid hyperactivation in acute liver injury. Hepatology Communications, 7(4), e0102.

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