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4 protocols using ikzf1 antibody

1

Antibody Characterization for PPAR, IKZF, CRBN

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The PPARα antibody was obtained from Santa Cruz Biotechnology, Dallas, TX, USA (Cat# SC-398394). The PPARβ/δ (NBP2-22468) and PPARγ (NBP2-22106) antibodies were obtained from Novus Biologicals, LLC. (Littleton, CO, USA). The IKZF1 antibody was purchased from Santa Cruz Biotechnology, Inc. (Dallas, TX, USA). The IKZF3 antibody was purchased from Novus. The CRBN antibody was obtained from Sigma-Aldrich (St. Louis, MO, USA). The Caspase 3 antibody, EZH2 antibody (#4905S), and H3K27me3 antibody (#9733T) were purchased from Cell Signaling Technology (Danvers, MA, USA).
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2

Antibody Procurement for PPARs and IKZFs

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PPARα antibody was obtained from Santa Cruz Biotechnology (Cat# SC-398394). PPARβ/δ (NBP2-22468) and PPARγ (NBP2-22106) antibodies were obtained from Novus Biologicals, LLC (Littleton, CO). IKZF1 antibody was purchased from Santa Cruz Biotechnology, Inc. (Dallas, TX, USA). IKZF3 antibody was purchased from Novus. CRBN antibody was obtained from Sigma-Aldrich (St. Louis, MO).
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3

Comprehensive Antibody Panel for Nuclear Receptors

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PPARα antibody was obtained from Santa Cruz (Cat#: SC-398394) and Abcam (Cat#: ab227074). PPAR-β/δ antibody was purchased from Novus Biologicals LLC (Cat#: NBP2-22468) and ABclonal (Cat#: A5656). PPARγ antibody was purchased from Novus Biologicals LLC (Cat#: NBP2-22106) and Thermo Fisher (Cat#: 16643-1-AP). IKZF1 antibody was purchased from Santa Cruz Biotechnology, Inc. (Dallas, TX, USA) and Novus Biologicals (Cat#: NBP1-98314). IKZF3 antibody was purchased from Novus (Cat#: NBP2-46048). CRBN antibody was obtained from Sigma-Aldrich (St. Louis, MO, USA). EZH2 antibody (Cat#4905S) and H3K27me3 antibody (Cat #9733T) were purchased form Cell Signaling Technology.
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4

Evaluating PIM and IKZF Inhibitors in MM

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CX6528, SGI1776, and lenalidomide were purchased from Selleckchem (Houston, TX). SGI1776 used for in vivo studies was provided by Tolero Pharmaceuticals, Inc (Lehi, Utah). Cycloheximide, bortezomib, and MG132 were purchased from Sigma-Aldrich (St. Louis, MO). Antibodies against PIM1, PIM2, PIM3 and ubiquitin were purchased from Abcam (Cambridge, MA). Antibodies against c-Myc, IRF4, pS6, and PARP were purchased from Cell Signaling (Danvers, MA). Annexin V and caspase 3 antibodies were purchased from BD Biosciences (San Jose, CA). IKZF1 antibody was purchased from Santa Cruz Biotechnology, Inc (Dallas, TX). IKZF3 antibody was purchased from Novus Biologicals LLC (Littleton, CO). Cereblon antibody was obtained from Sigma-Aldrich (St. Louis, MO).
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