Mrpl44

Manufactured by Proteintech

MRPL44 is a nucleic acid-binding protein that is involved in the translation of mitochondrial ribosomes. It is a component of the large subunit of the mitochondrial ribosome and plays a role in the regulation of mitochondrial protein synthesis.

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Lab products found in correlation

Phospho s6 ribosomal protein, by Cell Signaling Technology (1 mentions) Bolt bis tris plus gel, by Thermo Fisher Scientific (1 mentions) Chemidoc touch, by Bio-Rad (1 mentions) P eif2α, by Thermo Fisher Scientific (1 mentions) Irdye 800cw goat anti rabbit igg, by LI COR (1 mentions) Irdye 680lt goat anti mouse igg, by LI COR (1 mentions) Odyssey blocking buffer, by LI COR (1 mentions) Odyssey infrared imaging system, by LI COR (1 mentions) Mrps27, by Proteintech (1 mentions)

2 protocols using mrpl44

Western Blot Analysis of Stress Markers

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HeLa cells or 293T cells were seeded in 12-well plate (2 × 10⁵ cells/ml) 24 hours before each experiment. Immediately after the indicated treatments, cells were lysed using 120 μL of 2 x loading buffer (50 mM Tris pH 6.8, 10% glycerol, 2% SDS, 0.05% bromophenol blue and 100 mM DTT), boiled at 95°C for 5 minutes and sonicated. 10 μL of extract were loaded on a 4%–12% Bolt Bis-Tris Plus gel (ThermoFisher Scientific).
Immunoblots were visualized using the Biorad ChemiDoc™ touch imaging system (Biorad).
The following antibodies were used: CHOP (Thermo Fisher Scientific, MA1-250, 1/500 dilution), p-eIF2α (ThermoFisher Scientific, 44-728G, 1/1000 dilution), tubulin (Sigma-Aldrich, T5168, 1/2000 dilution), MRPL44 (Proteintech, 16394-1-AP, 1/1000 dilution), MRPL28 (Abcam, ab126719, 1/1000 dilution), RPL4 (Proteintech, 11302-1-AP, 1/1000 dilution), RPS6 (Cell Signaling, 2217S, 1/1000 dilution), HSPC014 (Abcam, ab170865, 1/1000 dilution) phospho-S6 ribosomal protein (Cell Signaling, 4856S, 1/1000 dilution) and ATF4 (Santa Cruz Biotechnology, sc-390063, 1/1000 dilution).

Schneider K., Nelson G.M., Watson J.L., Morf J., Dalglish M., Luh L.M., Weber A, & Bertolotti A. (2020). Protein Stability Buffers the Cost of Translation Attenuation following eIF2α Phosphorylation. Cell Reports, 32(11), 108154.

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Mitochondrial Protein Detection via Western Blot

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Specific proteins were detected using rabbit polyclonal antibodies against: MRPL11, MRPS15, porin, NDUFA9 (a subunit of Complex I), Complex II (SDHA), COX1, COXII, COXIV and Complex V subunit α (Abcam diluted 1:1000), AUH (Sigma, diluted 1:500) and MRPS27 (Proteintech, diluted 1:500) and mouse monoclonal antibodies against: MRPS35 (Sigma, diluted 1:1000), MRPL44 (Proteintech, diluted 1:1000), MRPL12 (Abnova, diluted 1:1000) in Odyssey Blocking Buffer (Li-Cor). IR Dye 800CW Goat Anti-Rabbit IgG or IRDye 680LT Goat Anti-Mouse IgG (Li-Cor) secondary antibodies were used and the immunoblots were visualized using an Odyssey Infrared Imaging System (Li-Cor).

Richman T.R., Davies S.M., Shearwood A.M., Ermer J.A., Scott L.H., Hibbs M.E., Rackham O, & Filipovska A. (2014). A bifunctional protein regulates mitochondrial protein synthesis. Nucleic Acids Research, 42(9), 5483-5494.

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