Usb2000 flg

Using a scanning electron microscope (SEM, Hitachi S‐3000N), the MNPs’ morphology and the hybrid films’ nanostructures were examined. An optical microscope (Olympus, BX51) fitted with a fiber‐optic (Ocean Optics, USB2000‐FLG) spectrometer was used to detect reflection spectra. The optical microscope was used to study the stained sample pieces (OLYMPUS BX51). ImageJ software was used to implement quantitative analysis of photographs.

The microscopic morphology of the colloidal crystal template, PLGA solution infused colloidal crystal template, PLGA scaffold with double layers of inverse opal structure, and the liquid paraffin infused inverse opal scaffolds were determined via SEM. The reflection spectra of the template, PLGA IO, and liquid paraffin infused PLGA IO, and the dynamic variation of the reflection spectra during the stretching of PLGA inverse opal scaffold and the hybrid patch was measured using a fiber optic spectrometer (Ocean Optics, USB2000‐FLG). To measure the mechanical properties, rectangular PLGA scaffold, PLGA inverse opal scaffold, and hybrid patch with the length, width, and thickness of 50, 30 mm and 200 µm were prepared. The maximum tensile stress and strain were recorded by the mechanical testing machine. To measure the tensile strength on the porcine skin, the rectangular PNH, hybrid patch without inverse opal structure, and hybrid patch with the length, width, and thickness of 20, 10 mm and 200 µm were fabricated. The tensile strain–stress curves, maximum tensile strength, and strain were recorded by using the mechanical testing machine.

The microscopic structures of the structural color hydrogels were magnified and scanned by a scanning electron microscope (Hitachi S‐3000N). A fiber‐optic spectrometer (Ocean Optics, USB2000‐FLG) was employed to measure the reflection spectra and test the optical properties of the functional hydrogel. As for the electrical performance measurement, a digital multimeter (KEITHLEY, USA) was utilized to record the resistance changes of the hydrogel during the bending process. The relationship between bending angle and stress was measured by MicroTester G2 (CellScale Biomaterials Testing, Waterloo, Canada) under the bending test mode. Fluorescent images of cardiomyocytes were obtained by a Zeiss LSM700 laser‐scanning microscope (Zeiss, Heidenheim, Germany). Optical images were derived by a CCD camera (Media Cybernetics Evolution MP5.0) equipped microscope (Olympus BX51). The mechanical properties were tested through a universal testing machine (Suns, UMT2000). The MTT assay results were scanned by a microplate reader whose model was SYNERGY|HTX.