Rabbit anti ve cadherin

The heart sections were incubated in blocking solution for 1 h at room temperature and then incubated at 4 °C overnight with one of the following antibodies: rabbit anti-VE-cadherin (1:200; Invitrogen Cat. #36-1900), goat anti-CD31 (1:200; R&D Cat. #AF3628), rabbit anti-zonula occludens-1 (1:200; Invitrogen Cat. #61-7300), rabbit anti-occludin (1:200; Invitrogen Cat. #71-1500), mouse anti-NG2 (1:100; Millipore Cat. #AB5320), rabbit anti-VCAM-1 (1:200; Santa Cruz Cat. #sc-13160), rabbit anti-MPO (1:50; Abcam Cat. #ab9535), mouse anti-CD68 (1:100; Abcam Cat. #ab955), rabbit anti-iNOS (1:200; Abcam Cat. #ab15323), rabbit anti-CD206 (1:100; Abcam Cat. #ab64693), or mouse anti-cTnT (1:200; Abcam Cat. #ab8295). Confocal images were captured at room temperature with ZEN software on an upright confocal microscope (LSM 700; Carl Zeiss) using the predefined ZEN software configurations for Alexa Fluor 546, Alexa Fluor 488, and DAPI.

The following is a list of the primary antibodies used and their respective dilutions were: mouse anti-YAP, 1:200 (Santa Cruz, cat. no. sc-271134); mouse anti-CK20, 1:100 (Dako, cat. no. M7019); rabbit anti-VE-cadherin, 1:2000 (Invitrogen, cat. no. PA5-19612), rabbit anti-pMLC P-Myosin Light Chain 2 (Thr18/Ser19), 1:200 (Cell Signaling cat. no. 3674), rabbit anti-. Ezrin/Radixin/Moesin, 1:100 (Cell Signaling cat. no. 3142), rabbit anti-phospho Ezrin (Thr567)/ Radixin (Thr564)/ Moesin (Thr558), 1:100 (Cell Signaling cat. no. 3141). The secondary antibodies used were: goat anti-mouse Alexa Fluor 488 (Thermo Fisher Scientific, cat. no. A-11029); donkey anti-rabbit Alexa Fluor 488 (Thermo Fisher Scientific, cat. no. A-21206), goat anti-rabbit Alexa Fluor 555 (Thermo Fisher Scientific, cat. no. A-21429) and goat anti-mouse Alexa Fluor 405 (Abcam, cat. no. ab175660). All the secondary antibodies were used at a dilution of 1:400. To label F-actin, phalloidin Atto 488 (Sigma-Aldrich cat. no. 49409) was used at 1:500 and phalloidin Alexa Fluor-647 (Thermo Fisher Scientific, cat. no. A22287) at 1:400. Hoechst (Thermo Fisher Scientific, cat. no. 33342) was used to label nuclei.