Between 0800 and 1100, rats were placed into custom, rectangular conditioning chambers (20”W x 10”D x 12”H; Context A) with a shock grid floor (Coulbourn Instruments, Allentown, PA) housed inside individual sound-attenuating cabinets illuminated by red lights. Rats were transported to Context A in their home cages. Once in Context A, rats were allowed to explore the context for 3 min, followed by 4 presentations of a 10 s, 2 KHz auditory CS that coterminated with a 1 s, 0.8mA foot shock US. Presentations of the auditory stimuli CS with an associated foot shock US were given on a 1-minute ITI. Auditory CS and foot shock US were delivered through Coulbourn tone generators and shock scramblers controlled via Noldus EthoVision XT software (Tacoma, WA) through a custom interface. All rats remained in the conditioning chamber for 1 min after the last shock before being transported back to the housing room in their home cages. Chambers were cleaned with water and dried between rats. All behavioral tests were recorded with overhead cameras for later scoring of freezing.
Shock grid floor
Manufactured by Harvard Apparatus
The Shock Grid Floor is a laboratory equipment designed to provide a controlled electrical stimulation to small animals in a contained environment. It consists of a grid of metal bars or wires that can deliver a mild electric current, allowing researchers to study behavioral responses or physiological reactions to the stimuli.
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Lab products found in correlation
2 protocols using shock grid floor
1
Auditory Cue Conditioning in Rats
2
Cue and Contextual Fear Conditioning
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At 3 months post-surgery, animals were trained and tested for cue and contextual fear conditioning [19 (link)]. Animals were first habituated for 10 min to a cage equipped with a shock grid floor (Coulbourn Instruments). At 24 hr after habituation, animals were trained by being placed in the apparatus for 120 s, and then a 30 s tone (75 dB, 2.8 kHz) was delivered that co-terminated with a 1 mA, 1 s foot shock. Animals remained in the apparatus for 60 s after the foot shock. At 24 hr and 1 month after training, animals were placed in the apparatus and freezing was measured for 5 min to assess contextual fear conditioning. Cue fear conditioning was evaluated 1 hr after assessment of contextual fear by placing the animals in an altered chamber. The tone (75 DB, 2.8 kHz) was played for 60 s and freezing was measured. Freezing behavior was quantified by video analysis (FreezeFrame 3.32, Coulbourn Instruments). Shock threshold was assessed 1 day after final cue and contextual fear conditioning testing (4 months post-surgery). Animals received a 1 s foot shock every 30 s in increments of 0.02 mA beginning at 0.1 mA. Minimum shock intensity to elicit a flinch, jump, or vocalization was measured.
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