The cytotoxicity of LNCaP and VCaP cells to agents was determined by the FITC-Annexin V/ PE-PI apoptosis detection kit (Becton and Dickinson Co., USA). Briefly, agents treated tumor cells were resuspended and stained with Annexin V/PI staining solution for 15 min at room temperature. Then cells apoptosis was analyzed on a C6 flow cytometer (Becton and Dickinson Co., USA). Each experiment was repeated for three independent times.
Fitc annexin 5 pe pi apoptosis detection kit
Manufactured by BD
Sourced in United States
The BD FITC-Annexin V/PE-PI Apoptosis Detection Kit is a laboratory reagent used for the detection and quantification of apoptosis in cells. The kit utilizes Annexin V, a calcium-dependent phospholipid-binding protein, conjugated to the fluorescent dye FITC, and propidium iodide (PI), a DNA-binding dye, to identify and differentiate between early apoptotic, late apoptotic, and necrotic cells.
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8 protocols using fitc annexin 5 pe pi apoptosis detection kit
1
Evaluating Apoptosis in Prostate Cancer Cells
2
Apoptosis Induction in Glioma Cells
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Cell apoptosis of LN229/T98G cells induced by TMZ or inhibitors was determined using the FITC-Annexin V/PE-PI apoptosis detection kit (Becton Dickinson, USA) according to the guidance of manufacturer. Briefly, LN229 and T98G cells were treated with TMZ (1 μg/ml) for 48 h. Then cells were stained with FITC-Annexin V/PE-PI staining solution for 20 min at room temperature. After that, cell apoptosis was detected by a C6 flow cytometer (Becton Dickinson, USA).
3
Apoptosis Quantification in Gastric Cancer
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Cell apoptosis was determined using the FITC-Annexin V/PE-PI apoptosis detection kit (Becton Dickinson, USA) according to the guidance of manufacturer. Briefly, SGC-7901 and BGC-823 cells were treated with 5-FU (1 µg/ml) or PTX (2 µg/ml) for 48 h. Then cells were stained with FITC-Annexin V/PE-PI staining solution for 15 min. Cell apoptosis was detected by a C6 flow cytometer (Becton Dickinson, USA).
4
Cytotoxicity Quantification of Prostate Cancer Cells
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The cytotoxicity of LNCaP and VCaP cells to agents was determined by the FITC-Annexin V/ PE-PI apoptosis detection kit (Becton and Dickinson Co., USA). Brie y, agents treated tumor cells were resuspended and stained with Annexin V/PI staining solution for 15 min at room temperature. Then cells apoptosis was analyzed on a C6 ow cytometer (Becton and Dickinson Co., USA). Each experiment was repeated for three independent times.
5
Apoptosis Analysis of Cancer Cells
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The cytotoxicity of A549 or PC-9 cells to chemotherapy or inhibitor was analyzed using the FITC-Annexin V/ PE-PI apoptosis detection kit (BD, NJ, USA). Briefly, agents treated tumor cells were resuspended and stained with FITC-Annexin V and PE-PI staining solution for 15 min. Then cells apoptosis was detected by flow cytometry on a C6 flow cytometer (BD, NJ, USA). Each experiment was repeated for three independent times.
6
Cytotoxicity Assessment of Sor and Sun
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Cytotoxicity of Sor and Sun to tumor cells was analyzed using the FITC-Annexin V/PE-PI apoptosis detection kit (BD, NJ, USA). Renca cells were treated with Sun (10 μM) or Sor (5 μM) for 48 hours. A498 were treated with Sor (20 μM) or Sun (15 μM) for 48 hours. Then tumor cells were collected and resuspended with 100 μl staining buffer after chemotherapy treatment. 5 μl FITC-Annexin V staining solution and 2 μl PE-PI staining solution were added into the cells. After incubation of 15 minutes at room temperature, the cells were washed with PBS and detected by flow cytometry on a C6 flow cytometer (BD, NJ, USA). Each experiment was performed in triplicate.
7
Apoptosis Analysis of Osteosarcoma Cells
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Cytotoxicity analysis was performed using a FITC-Annexin V/PE-PI apoptosis detection kit (BD, NJ, USA) according to the manufacturer’s instructions. Briefly, after treatment with 75 mM MTX or 40 μM CIS for 48 h, osteosarcoma cells were stained with FITC-Annexin V and PE-PI staining solution. Apoptosis was detected on a C6 flow cytometer (BD, NJ, USA). Each experiment was repeated independently in triplicate.
8
Apoptosis Detection in Osteosarcoma Cells
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Cytotoxicity analysis was performed using the FITC-Annexin V/ PE-PI apoptosis detection kit (BD, NJ, USA) under the manufacturer's instructions. Brie y, after treatment with 75 mM MTX or 40 μM CIS for 48 hours, osteosarcoma cells were stained with FITC-Annexin V and PE-PI staining solution. Apoptosis was detected on a C6 ow cytometer (BD, NJ, USA). Each experiment was repeated independently in triplicate.
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