5‐ethynyl‐20‐deoxyuridine (EdU) incorporation assay kit (Ribobio, Guangzhou, China) was used according to manufacturer's instruction. 1× 104 cells per well were seeded in a 96‐well plate and incubated overnight and then replaced by 0, 30, 60 μmol L−1 MPA, respectively. After 48‐h incubation, 50‐μmol L−1 EdU was added for 4 hour before fixation, permeabilization, and staining. Finally, cell nuclei were stained with 1 × Hoechst nuclear dye for 30 min and then detected by fluorescence microscopy.
5 ethynyl 20 deoxyuridine edu incorporation assay kit
Manufactured by RiboBio
Sourced in China
The 5‐ethynyl‐20‐deoxyuridine (EdU) incorporation assay kit is a laboratory tool used to detect and quantify DNA synthesis in cells. The kit provides a method to label and detect proliferating cells by incorporating the thymidine analog EdU into the DNA during DNA replication.
Automatically generated - may contain errors
Lab products found in correlation
3 protocols using 5 ethynyl 20 deoxyuridine edu incorporation assay kit
1
EdU Proliferation Assay in Cells
2
Cell Viability, Proliferation, and Cell Cycle Assays
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cell viability was determined using the colony formation assay, 3‐(4, 5‐dimethylthiazol‐2‐yl)‐2, 5‐diphenyltetrazolium bromide (MTT) assay (Sigma, St. Louis, MO, USA) and Celltiter‐Glo assay (Promega, Madison, WI, USA). The 5‐ethynyl‐20‐deoxyuridine (EdU) incorporation assay kit (RiboBio) was used to detect nuclear replication according to the manufacturer’s instructions. Propidium iodide (PI) was utilized to stain ethanol‐treated cells. Next, the cell cycle distribution was detected using a FACSCalibur flow cytometer (BD Biosciences, Franklin Lakes, NJ, USA).
3
Cytotoxicity and Apoptosis Evaluation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Xn was obtained from Sigma-Aldrich; Merck KGaA (St. Louis, MO, USA). CellTiter 96® AQueous One Solution Cell Proliferation Assay kit was purchased from Promega Corporation (Madison, WI, USA). The 5-ethynyl-20-deoxyuridine (EdU) incorporation assay kit was purchased from RiboBio (Guangzhou China), the FITC Annexin V Apoptosis Detection Kit I was obtained from BD Pharmingen (BD Biosciences, Franklin Lakes, NJ, USA). Antibodies against Bcl-2 (rabbit polyclonal antibody, dilution 1:1,000; cat. no. ab194583), Bax (rabbit monoclonal antibody, dilution 1:1,000; cat. no. ab32503), p-IκBα (rabbit monoclonal antibody, dilution 1:1,000; cat. no. ab133462), IκBα (rabbit monoclonal antibody, dilution 1:1,000; cat. no. ab32518), p65 (rabbit polyclonal antibody, dilution 1:1,000; cat. no. ab16502), histone H3 (rabbit polyclonal antibody, dilution 1:1,000; cat. no. ab1791) and GAPDH (rabbit polyclonal antibody, dilution 1:1,000; cat. no. ab9485) were obtained from Abcam (Cambridge, UK). ROS and superoxide dismutase (SOD) assay kits were purchased from Beyotime Institute of Biotechnology, Ltd. (Shanghai, China).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!