Cortecs uplc t3 column

Manufactured by Waters Corporation

Sourced in United States

The CORTECS UPLC T3 column is a reversed-phase high-performance liquid chromatography (HPLC) column designed for the separation and analysis of various compounds. The column features a stationary phase made of 1.6 μm core-shell particles, which provide efficient chromatographic separation and high-resolution performance.

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Lab products found in correlation

Ultra performance liquid chromatography (uplc), by Waters Corporation (1 mentions) Synapt g2 si ion mobility mass spectrometer, by Waters Corporation (1 mentions) Masslynx 4.1 system, by Waters Corporation (1 mentions) Valuelab, by Agilent Technologies (1 mentions) Tq s mass spectrometer, by Waters Corporation (1 mentions)

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CORTECS T3 column (13 citations) CORTECS UPLC T3 (7 citations) Cortecs T3 (4 citations)

4 protocols using cortecs uplc t3 column

UPLC-MS Analysis of Compound CZX

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Qualitative analysis of CZX was performed by UPLC (Waters, Milford, MA, USA). The chromatographic separation was performed on a Waters CORTECS UPLC T3 column (2.1 × 100 mm, 1.6 μm). The mobile phase was composed of 0.1% aqueous formic acid solution (A) and acetonitrile (B). The gradient elution conditions were as follows: 0 to 2 min, 5% B; 2 to 32 min, 5% to 100% B; 32 to 33 min, 100% B; 33.5 min, 5% B; 33.5 to 35 min, 5% B. The column temperature was set at 35°C, the flow rate was 0.3 mL/min, and the injection volume was 2 μL.
Electrospray ionization (ESI) sources were used to acquire mass spectrometry data from 50 m/z to 1200 m/z in both negative and positive ionization modes. The source parameters were as follows: ion source temperature of 120°C, desolvation temperature of 400 to 500°C, capillary voltage of 2.5 to 3.0 kV, sample cone voltage of 40 V, source offset voltage of 80 V, low collision energy of 6 V, high collision energy of 15 to 45 V, and desolvation gas flow rate of 800 to 1000 L/h.

Fang T., Xu R., Sun S., He Y., Yan Y., Fu H., Luo H., Cao Y, & Tao M. (2023). Caizhixuan hair tonic regulates both apoptosis and the PI3K/Akt pathway to treat androgenetic alopecia. PLOS ONE, 18(2), e0282427.

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UPLC-Q/TOF-MS Analysis of WECP Metabolites

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The UPLC-Q/TOF-MS system was used to measure the mass of each secondary metabolites in the WECP. The dried WECP powder was reconstituted in desalinated water to obtain 1 mg/mL WECP aqueous solution. Then 2 μL was injected into a CORTECS UPLC T3 column (2.1 mm × 100 mm; 1.6 μm) (Waters Corp. Milford, MA, United States) fitted with the UPLC system (Waters Corp.). The mobile phase was a mixture of acetonitrile (A) and 0.1% (v/v) formic acid B). The elution program was set as follows: 0–2.00 min, 5% A; 2.01–32.00 min, 5%–100% A; and 32.01–35.00 min, 5% A. The flow rate was set as 0.3 mL/min. The mass of each ingredient was measured by SYNAPT G2-Si ion mobility mass spectrometry (Waters Corp.). Electrospray ionization mass spectrometry was performed in positive and negative modes. The mass-to-charge ratio (m/z) scan range was 50–1,200. Finally, the results were comparatively analyzed using SCIEX OS software.

Fu H., Li W., Weng Z., Huang Z., Liu J., Mao Q, & Ding B. (2023). Water extract of cacumen platycladi promotes hair growth through the Akt/GSK3β/β-catenin signaling pathway. Frontiers in Pharmacology, 14, 1038039.

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UPLC-TOF-MS Analysis of QB Decoction

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The main contents of QB decoction were analyzed by ultrahigh-performance liquid chromatography time of flight mass spectrometry (UPLC-TOF-MS) for quality control. The temperature was settled at 35°C and the chromatographic column used was a CORTECS UPLC T3 column (2.1 mm × 100 mm, 1.67 μm) (Waters, United States). The sample volume was 2 μl, and the flow rate was 1.0 ml/min. The mobile phases were a mixture of acetonitrile (A) and 0.1% acetic acid (B). The gradient elution program was used: 0–30.00 min, 10%–100% A; 30.01–31.00 min, 100%–10% A; 31.01–35.00 min, 10%–100% A.
The UPLC-TOF-MS used SYNAPT G2-Si ion mobility mass spectrometer (Waters, United States). The electrospray ionization (ESI) was used in positive and negative mode, and the mass-to-charge ratio (m/z) scan range was set at 50–1,000 Da. Mass spectrometry data were collected in centroid MS^E mode. The relative molecular mass accuracy was automatically calibrated using the tuned liquid transfer system from Waters. The instrument operation and data acquisition were controlled by the Waters MassLynx 4.1 system, and the compounds were identified according to the relative retention time and mass spectrometry information of each compound.

Hong J., Zhang M., He Y., Jin Y., He Q., Zhang Y., Shi X., Tian W., Wen C, & Chen J. (2022). Qinghao-Biejia Herb Pair Alleviates Pristane-Induced Lupus-Like Disease and Associated Renal and Aortic Lesions in ApoE−/− Mice. Frontiers in Pharmacology, 13, 897669.

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Quantifying c-di-GMP in Streptomyces

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Streptomyces samples were collected after being cultured for 1 day, 2 days, 3 days, or 4 days in GYM medium. The Streptomyces samples were centrifuged at 10,000 rpm and the strains were collected and frozen in liquid nitrogen, and then ground into fine powder. Then, the powder was incubated with 2 M formic acid in ice-cold water for 30 min, followed by the addition of ammonium acetate (50 mM, pH of 4.5). The supernatants were harvested by centrifugation at 5000× g for 10 min at 4 °C. The solid-phase extraction column (Welch) was prepared by adding 1 mL of methanol (MeOH) and 1 mL of 50 mM ammonium acetate (NH₄OAC) (pH of 4.5), followed by the addition of supernatant and washing of the column with 1 mL of 50 mM NH₄OAC (pH of 4.5) and 1 mL of MeOH. The c-di-GMP in the SPE column was eluted with 1 mL of a methanol/H₂O/ammonium hydroxide (20:70:10) solution. The collected liquid was blown dry with nitrogen, subsequently dissolved in 200 µL of water, and filtered through a 0.2 µm filter (Agilent ValueLab) into a collection tube. The samples were analyzed using an ACQUITY UPLC I-Class ultra-high-performance liquid chromatography (HPLC) system and a Xevo TQ-S mass spectrometer (Milford, MA, USA) using a CORTECS UPLC T3 column (Waters, 1.6 μm 2.1 × 150 mm). The c-di-GMP analysis was determined according to the method described by Makitrynskyy et al. [16 (link)].

Wang R., Zhang Z., Yu X., Song Y, & Shentu X. (2024). CdgB Regulates Morphological Differentiation and Toyocamycin Production in Streptomyces diastatochromogenes 1628. International Journal of Molecular Sciences, 25(7), 3878.

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