Leupeptin, pepstain, aprotinin, phenylmethylsulfonyl fluoride (PMSF), dithiothreitol (DTT), Triton X-100 (TX100), sodium dodecyl sulfate (SDS), propidium iodide and other chemicals, as well as antibodies to beta-actin, Iba-1, and GFAP were purchased from Sigma (St. Louis, MO). Antibodies to Hsp40, Hsp70, Hsp90, Grp78, Grp94, calnexin, and PDI were purchased from Stressgen Biotechnologies, Inc. (San Diego, CA). Active caspase 3 and HSF1 antibodies were purchased from Cell Signaling Tech (Danvers, MA). Secondary antibodies were purchased from Jackson ImmunoResearch Labs (West Grove, PA).
Grp94
Manufactured by Stressgen
GRP94 is a laboratory equipment product that serves as a molecular chaperone protein. It plays a key role in the folding and stabilization of proteins within the endoplasmic reticulum of cells.
Automatically generated - may contain errors
Lab products found in correlation
Grp78, by Stressgen (2 mentions)
Pepstain, by Merck Group (1 mentions)
Propidium iodide, by Merck Group (1 mentions)
Sodium dodecyl sulfate (sds), by Merck Group (1 mentions)
Aprotinin, by Merck Group (1 mentions)
Leupeptin, by Merck Group (1 mentions)
Calnexin, by Stressgen (1 mentions)
Hsp70, by Stressgen (1 mentions)
Hsp90, by Stressgen (1 mentions)
Quantity one system, by Bio-Rad (1 mentions)
β actin, by Merck Group (1 mentions)
Carboxyfluorescein succinimidyl ester (cfse), by BioLegend (1 mentions)
Phosphotyrosine 4g10, by Merck Group (1 mentions)
Phospho syk tyr525 526, by Cell Signaling Technology (1 mentions)
Phospho akt ser473, by Thermo Fisher Scientific (1 mentions)
Phospho erk1 2 thr202 tyr204, by Cell Signaling Technology (1 mentions)
Erk1 2, by Cell Signaling Technology (1 mentions)
Lipopolysaccharides (lps), by Merck Group (1 mentions)
3 protocols using grp94
1
Protein Extraction and Detection
2
Protein Extraction and Western Blot Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Lysates of tissues from individual mice were extracted by 3 freeze-thaw cycles followed by homogenization in ice-cold radioimmunoprecipitation assay buffer (50 mmol/l Tris-Cl, 150 mmol/l NaCl, 1% NP-40, 0.5% sodium deoxycholate, and 0.1% SDS) containing cocktails of proteinase inhibitors and phosphatase inhibitors (Roche), and centrifuged for 15 min at 13,000 g. Proteins were separated by 8% or 12% SDS-PAGE, transferred to nitrocellulose membrane (Pall), and subjected to Western blotting10 (link). Primary antibodies included GRP78, GRP94, CNX and CRT (1:2,000; Stressgen), and β-actin (1:5,000; Sigma). Western blotting was repeated two to four times and signal intensities were measured using the Quantity One system (Bio-Rad).
3
Polyclonal Antibody Generation and Reagent Sources
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