Pc g5 tdt

Manufactured by Jackson ImmunoResearch

PC::G5-tdT is a fluorescent protein product offered by Jackson ImmunoResearch. It is a tandem dimer of the red fluorescent protein tdTomato, which can be used for various biological applications requiring a bright red fluorescent signal.

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Lab products found in correlation

Prrx1 cre, by Jackson ImmunoResearch (1 mentions) Piezo2 egfp ires cre, by Jackson ImmunoResearch (1 mentions) Rosa26lsl yfp, by Jackson ImmunoResearch (1 mentions) S100a8 cre, by Jackson ImmunoResearch (1 mentions)

2 protocols using pc g5 tdt

Piezo Mechanosensitive Ion Channels in Mice

Cited 2 times

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All animal experiments were carried out according to protocols approved by the Harvard Medical School Institutional Animal Care and Use Committee. Mice described in the literature and purchased from the Jackson Laboratories: Piezo1^P1-tdT (stock# 029214) (Ranade et al., 2014 (link)), Piezo2-EGFP-IRES-Cre (Piezo2^{tm1.1(cre)Apat}, stock# 027719) (Woo et al., 2014 (link)), Piezo1^f/f (stock# 029213) (Cahalan et al., 2015 (link)), Piezo2^f/f (stock# 027720) (Woo et al., 2014 (link)), Prrx1-Cre (stock# 005584) (Logan et al., 2002 (link)), Sp7-GFP::Cre (stock# 006361) (Rodda and McMahon, 2006 (link)), Polr2a^{Tn(pb-CAG-GCaMP5g,-tdTomato)Tvrd} (PC::G5-tdT, stock# 024477) (Gee et al., 2014 (link)), Ai9 (Rosa26-TdTomato, stock# 007909). For embryos or neonatal mice, both male and female were used in the analyses as sex could not be clearly identified in embryos or neonatal mice. Sex-matched littermate mice were compared in postnatal mice at day 21. Three or more littermate groups were examined and representative images are shown.

Zhou T., Gao B., Fan Y., Liu Y., Feng S., Cong Q., Zhang X., Zhou Y., Yadav P.S., Lin J., Wu N., Zhao L., Huang D., Zhou S., Su P, & Yang Y. (2020). Piezo1/2 mediate mechanotransduction essential for bone formation through concerted activation of NFAT-YAP1-ß-catenin. eLife, 9, e52779.

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Genetically Engineered Mouse Models

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All in vivo mouse experimental procedures were performed under the Sloan Kettering Institute (SKI) Institutional Animal Care and Utilization Committee (IACUC) - approved protocol 07-06-008. Mice were housed in specific pathogen-free animal facilities. Only female mice were used in this study. Littermates were used in all experiments, when possible, otherwise age-matched cage mates were used. Itgax-Cre, S100a4-Cre, S100a8-Cre, Rosa26^LSL-YFP, Cdh1^mCFP and GCaMP5 (also known as PC-G5-tdT) mice were obtained from Jackson Laboratories. Gzmc^tdT-T2A-iCre mice were generated in the Li lab^{32 (link)}. IL-15^2A-eGFP mice were previously described^{28 (link)}. Il15^fl mice were generated in the Ikuta lab and will be reported elsewhere. Briefly, the Il15^fl allele was established by flanking exon 5 with two loxP sequences by standard homologous recombination in embryonic stem cells (schematic provided in Extended Data Fig. 6c).

Kansler E.R., Dadi S., Krishna C., Nixon B.G., Stamatiades E.G., Liu M., Kuo F., Zhang J., Zhang X., Capistrano K., Blum K.A., Weiss K., Kedl R.M., Cui G., Ikuta K., Chan T.A., Leslie C.S., Hakimi A.A, & Li M.O. (2022). Cytotoxic Innate Lymphoid Cells Sense Cancer Cell-Expressed Interleukin-15 to Suppress Human and Murine Malignancies. Nature immunology, 23(6), 904-915.

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