Clone e7k2y

Manufactured by Cell Signaling Technology

Clone E7K2Y is a lab equipment product offered by Cell Signaling Technology. It serves as a core functional component, but a detailed description while maintaining an unbiased and factual approach is not available.

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Lab products found in correlation

Alexa fluor 488 goat anti rabbit igg, by Thermo Fisher Scientific (1 mentions) Axio imager 2, by Zeiss (1 mentions) Prolong diamond antifade, by Thermo Fisher Scientific (1 mentions) Antigen retrieval buffer, by Agilent Technologies (1 mentions) Alexa fluor 555 goat anti rabbit igg, by Thermo Fisher Scientific (1 mentions) Fab13501p, by R&D Systems (1 mentions) Clone 1c12, by Cell Signaling Technology (1 mentions)

Spelling variants of this product

E7K2Y (2 citations)

2 protocols using clone e7k2y

Immunostaining of DU-145 Xenograft Tissues

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Formalin-fixed paraffin-embedded DU-145 xenograft tissue sections were deparaffinized, rehydrated and subject to heat-induced antigen retrieval using antigen retrieval buffer (pH 6.0; Dako, Carpinteria, CA) in a pressure cooker. Sections were blocked with 2.5% normal horse serum for 30 min and incubated with mixture of rabbit monoclonal anti-CD133 antibodies (clone D2V8Q, diluted 1:100; Cell signaling, Danvers, MA) and mouse monoclonal anti-TRA (clone TRA-1-60 (S), diluted 1:1000; Cell signaling) or mixture of rabbit monoclonal anti-CD44 antibodies (clone E7K2Y, diluted 1:1000; Cell signaling) and mouse monoclonal anti-TRA (clone TRA-1-60 (S), diluted 1:1000; Cell signaling) for 1 h at room temperature, followed by incubation with mixture of Alexa Fluor 488 goat anti-rabbit IgG (ThermoFisher Scientific, Waltham, MA) and Alexa Fluor 555 goat anti-rabbit IgG (ThermoFisher Scientific). After nucleus visualization with DAPI, sections were mounted with Prolong Diamond antifade (ThermoFisher Scientific) and imaged on a Zeiss Axio Imager 2 (Carl Zeiss Microscopy, Jena, Germany).

White J.M., Ramos N., Saliganan A.D., Chung J.Y., Bell M., Lindquist J., Conner K., Wiesend W.N., Schopperle M., Patrick S.M., Kim S., Heath E.I., Escorcia F.E, & Viola N.T. (2023). Selective ablation of TRA-1-60+ pluripotent stem cells suppresses tumor growth of prostate cancer. Theranostics, 13(7), 2057-2071.

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Comprehensive Immunophenotyping Protocol

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Antibodies used for FACS: Human/mouse/bovine integrin alpha 6/CD49f PE-conjugated antibody (FAB13501P, R&D Systems, 1:40); PE/Cyanine7 anti-mouse CD326 (Ep-CAM) antibody (118216, BioLegend, 1:40); CD31 (PECAM-1) monoclonal antibody (390), FITC (11-0311-82, eBioscience, 1:100); CD45 monoclonal antibody (30-F11), FITC (11-0451-85, eBioscience, 1:100); TER-119 monoclonal antibody (TER-119), FITC (11-5921-82, eBioscience, 1:100). Antibodies used for immunohistochemistry: Anti-wide spectrum Cytokeratin antibody (ab9377, Abcam, 1:100); rabbit monoclonal GFP antibody (clone D5.1, Cell Signaling, 1:100); rabbit polyclonal p63 antibody (12143-1-AP, Proteintech, 1:200); mouse monoclonal p53 antibody (clone 1C12, Cell Signaling, 1:500); rabbit monoclonal HOXB13 antibody (clone D7N8O, Cell Signaling, 1:50); rabbit polyclonal AR antibody (06-680, Millipore, 1:2,000); rabbit monoclonal GATA3 antibody (clone D13C9, Cell Signaling, 1:200); rabbit monoclonal CD44 antibody (clone E7K2Y, Cell Signaling, 1:100).

Li S., Wong A., Sun H., Bhatia V., Javier G., Jana S., Wu Q., Montgomery R.B., Wright J.L., Lam H.M., Hsieh A.C., Faltas B.M., Haffner M.C, & Lee J.K. (2024). A combinatorial genetic strategy for exploring complex genotype–phenotype associations in cancer. Nature Genetics, 56(3), 371-376.

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