Low density lipoprotein cholesterol assay kit

Manufactured by Nanjing Jiancheng

Sourced in China

The Low Density Lipoprotein Cholesterol Assay kit is a laboratory tool designed to quantify the concentration of low-density lipoprotein cholesterol in biological samples. It provides a standardized method for the analytical measurement of this specific lipid component.

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13 protocols using low density lipoprotein cholesterol assay kit

Antibody and Assay Reagents for Metabolic Studies

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GnRH antibody (ab16216) and IL-1β antibody (ab9722) were purchased from Abcam (Cambridge, UK). NF-κB p65(D14E12, Catalog number:8242) was purchased from Cell Signaling Technology, Inc (Boston, USA). Iba1 antibody (Catalog number: 10904-1-AP), GFAP antibody (Catalog number:16825-1-AP), SIRT1 antibody (Catalog number:13161-1-AP), TNF-α antibody (Catalog number:17590-1-AP), GAPDH (Catalog number:60004-1-Ig) were purchased from Proteintech Group Inc (Wuhan, China). Total cholesterol assay kit (Catalog number:A111-1-1), Triglyceride assay kit (Catalog number:A110-1-1), Low-density lipoprotein cholesterol assay kit (Catalog number:A113-1-1), High-density lipoprotein cholesterol assay kit (Catalog number:A112-1-1) and Glucose assay kit (Catalog number:F006-1-1) were purchased from Nanjing Jiancheng Bioengineering Institute (Nanjing, China). ELISA Kits of mouse MMP-2, MMP-9, TNF-α and IL-1β (The catalog numbers: EK0460, EK0466, EK0527, EK0394) were purchased from BOSTER Biological Technology.co.ltd (Wuhan, China). High-fat diet (MD12015) was purchased from Jiangsu Medison Biomedical Co., Ltd (Yangzhou, China). Elisa kit of SIRT1 was purchased from Enzyme-free Biotechnology Co., Ltd (Jiangsu, China).

Wang X., Yang J., Lu T., Zhan Z., Wei W., Lyu X., Jiang Y, & Xue X. (2020). The effect of swimming exercise and diet on the hypothalamic inflammation of ApoE-/- mice based on SIRT1-NF-κB-GnRH expression. Aging (Albany NY), 12(11), 11085-11099.

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Enzymatic Lipid Profiling Protocol

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Enzymatic biochemical kits (Nanjing Jiancheng) were used to determine serum lipids via microplate reader (TECAN): Total Cholesterol Assay kit (Cat A111-1), Triacylglycerol Assay kit (Cat A110-1), Low Density Lipoprotein Cholesterol Assay kit (Cat A113-1), and High Density Lipoprotein Cholesterol (Cat A112-1).

Qiu R., Long J., Zhou L., Ma Y., Zhao L., Liu F, & Yuan D. (2019). Yangyin Qingre Huoxue Method in Traditional Chinese Medicine Ameliorates Atherosclerosis in ApoE−/− Mice Suffering from High-Fat Diet and HSP65 Aggression. Evidence-based Complementary and Alternative Medicine : eCAM, 2019, 2531979.

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Uterine Weight and Serum Biomarkers in Rats

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The rats were weighed every week. At the end of the experiments, the rats were sacrificed by cardiac puncture under anesthesia. Their uteruses were removed and weighed. The uteruses were fixed in 10% neutral formalin then embedded in paraffin. Sections of 5 μm were cut by a slicing machine for HE staining. The blood was centrifuged at 3000 rpm at 4°C for 10 mins, and the supernatant was carefully transferred to another tube and stored at −80°C until analysis. The level of triglycerides (TG), total cholesterol (TC), high density lipoprotein (HDL), low density lipoprotein (LDL), glutathione (GSH) and superoxide dismutase (SOD) in the serum of the rats was measured by using a Triglycerides Assay Kit, Total Cholesterol Assay Kit, High-Density Lipoprotein Cholesterol Assay Kit, Low-Density Lipoprotein Cholesterol Assay Kit, Reduced Glutathione Assay Kit, and Superoxide Dismutase Assay Kit (WST-1 method) (Nanjing Jiancheng Bioengineering Institute, Nanjing, China). Absorbance (the reading at 450 nm or 560 nm) of each biochemical marker was read and compared across the groups.

Bei J., Zhang X., Wu J., Hu Z., Xu B., Lin S., Cui L., Wu T, & Zou L. (2018). Ginsenoside Rb1 does not halt osteoporotic bone loss in ovariectomized rats. PLoS ONE, 13(9), e0202885.

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Comprehensive Lipid Profile and Metabolomic Analysis

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Non-esterified fatty acids Kit (A042-2-1)/Triglycerides Assay Kit (F001-1-1)/Total cholesterol Assay Kit (F002-1-1)/High density lipoprotein cholesterol Assay Kit (F003-1-1)/Low-density lipoprotein cholesterol assay kit/(A113-1-1) from Nanjing Jiancheng Bioengineering Institute, China, was used to detect the concentration of FFA/TG/TC/HDL/LDL in the serum of patients. The serum concentration of GLU in the patient’s serum was measured using the Glucose Assay Kit (CAT: 361500) of Shanghai Rongsheng Biopharmaceutical Co., Ltd., China. The metabolomic platform of Tsinghua University in China used Liquid chromatography-mass spectrometry to quantitatively analyze various fatty acid components in the serum of all PCa patients and normal individuals.

Ha X., Wang J., Chen K., Deng Y., Zhang X., Feng J., Li X., Zhu J., Ma Y., Qiu T., Wang C., Xie J, & Zhang J. (2020). Free Fatty Acids Promote the Development of Prostate Cancer by Upregulating Peroxisome Proliferator-Activated Receptor Gamma. Cancer Management and Research, 12, 1355-1369.

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Molecular Markers in Cardiovascular Disease

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The antibodies against MiD51, Drp1, p-Drp1, ERK1/2, p-ERK1/2, p27^Kip1, Cyclin D1, VCAM-1, ICAM-1, HIF-1α and CD31 were purchased from Proteintech (Wuhan, China). Bovine Serum Albumin (BSA) and 4, 6-diamidino-2-phenylindole (DAPI) were obtained from Abcam Inc. (Cambridgeshire, UK). Synthetic oligonucleotide siMiD51-690, siMiD51-858, siMiD51-1021 and miR-107 mimics, and negative controls were purchased from Genepharma (Suzhou, China). Goat anti-Rabbit IgG (H + L) and anti-Mouse IgG (H + L) secondary antibodies were purchased from Thermo Scientific (Waltham, MA, USA). A total cholesterol assay kit, triglyceride assay kit, high-density lipoprotein cholesterol assay kit and low-density lipoprotein cholesterol assay kit were purchased from Jiancheng Bioengineering Institute (Nanjing, China).

Ren J., Liu J., Zhang J., Hu X., Cui Y., Wei X., Ma Y., Li X, & Zhao Y. (2023). Dynamin-Related Protein 1 Binding Partners MiD49 and MiD51 Increased Mitochondrial Fission In Vitro and Atherosclerosis in High-Fat-Diet-Fed ApoE-/- Mice. International Journal of Molecular Sciences, 25(1), 244.

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Cholesterol Quantification in Liver and Cells

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Plasma of total cholesterol, HDL-C, ApoA-I, and LDL-C was detected total cholesterol assay kit, high-density lipoprotein cholesterol assay kit, Apolipoprotein A-I Assay Kit, and low-density lipoprotein cholesterol assay kit (Nanjing Jiancheng Bioengineering Institute). The free cholesterol and total cholesterol in liver and cells were detected by gas chromatography–mass spectrometry (GC-MS). Liver tissues (10 mg–20 mg) and hepatocytes (1 × 10⁶) were homogenized in 1× PBS. Cholesterol was extracted at m/z = 329, 368, 458, and Internal standard (IS) was extracted at m/z = 217, 357 by GC chromatograms.

Li L., Zhong S., Li R., Liang N., Zhang L., Xia S., Xu X., Chen X., Chen S., Tao Y, & Yin H. (2022). Aldehyde dehydrogenase 2 and PARP1 interaction modulates hepatic HDL biogenesis by LXRα-mediated ABCA1 expression. JCI Insight, 7(7), e155869.

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Serum Lipid and Enzyme Profile Evaluation

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The levels of serum total cholesterol (TC), triglyceride (TG), glutamic-oxaloacetic transaminase (AST), glutamic-pyruvic transaminase (ALT), low-density lipoprotein (LDL), and high-density lipoprotein (HDL) were evaluated using the TG Triglyceride Kit (single agent GPO-PAP method, Jiancheng, Nanjing, China, A110-1-1), Total cholesterol assay kit (single agent GPO-PAP method, Jiancheng, Nanjing, China, A111-1-1), Alanine aminotransferase assay kit (Reitman Frankel assay, Jiancheng, Nanjing, China, C009-2-1), Aspartate aminotransferase assay kit (Jiancheng, Nanjing, China, C010-2-1), low-density lipoprotein cholesterol assay kit (Jiancheng, Nanjing, China, A113-1-1), and high-density lipoprotein cholesterol assay kit (Jiancheng, Nanjing, China, A112-1-1) respectively.

Yang J.W., Zou Y., Chen J., Cui C., Song J., Yang M.M., Gao J., Hu H.Q., Xia L.Q., Wang L.M., Lv X.Y., Chen L, & Hou X.G. (2023). Didymin alleviates metabolic dysfunction-associated fatty liver disease (MAFLD) via the stimulation of Sirt1-mediated lipophagy and mitochondrial biogenesis. Journal of Translational Medicine, 21, 921.

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Serum Lipid Profile Determination

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The blood samples were collected via retro‐orbital collection method, followed by coagulation at room temperature. Subsequently, the samples were centrifuged at 3500 g for 15 min to separate the serum. The concentrations of total cholesterol (T‐CHO), low‐density lipoprotein cholesterol (LDL‐C), and high‐density lipoprotein cholesterol (HDL‐C) were determined using the total cholesterol assay kit (Jiancheng, A111‐1), low‐density lipoprotein cholesterol assay kit (Jiancheng, A113‐1), and high‐density lipoprotein cholesterol assay kit (Jiancheng, A112‐1), respectively, following the manufacturer's instructions.

Tian X., Yin Z., Li Z., Wang Z., Xing Z., Liu C., Wang L., Wang C., Zhang J, & Dong L. (2023). Regeneration of Thyroid Glands in the Spleen Restores Homeostasis in Thyroidectomy Mice. Advanced Science, 11(6), 2305913.

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Molecular Mechanisms of PDGF-BB Signaling

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PDGF‐BB was purchased from PeproTech (100‐14B, Rocky Hill). GAPDH monoclonal antibodies (60004‐1‐Ig), KLF4 polyclonal antibodies (11880‐1‐AP) and STAT3 polyclonal antibodies (10253‐2‐AP) were purchased from Proteintech. Anti‐P‐STAT3 (Ser 727) Rabbit pAb (WL03346) were purchased from Wanleibio. MLCK monoclonal antibodies (ab76092), ML‐7 (ab120848), Y‐27632 dihydrochloride (ab120129) and 4,6‐diamidino‐2‐phenylindole (DAPI, ab104139) were obtained from Abcam Inc. The STAT3 inhibitor S3I‐201 was obtained from Selleck Chemicals (S1155). FITC‐conjugated α‐smooth muscle actin antibody (F3777) and Oil‐Red‐O solution (O1391) was purchased from Sigma‐Aldrich (St Louis, MO). Rhodamine conjugated goat anti‐rabbit IgG (H+L) (31670) was purchased from Thermo Scientific. Total cholesterol assay kit, Triglyceride assay kit, High‐density lipoprotein cholesterol assay kit, Low‐density lipoprotein cholesterol assay kit were purchased from Nanjing Jiancheng Bioengineering Institute (China).

Wang J., Zhang C., Li C., Zhao D., Li S., Ma L., Cui Y., Wei X., Zhao Y, & Gao Y. (2019). MicroRNA‐92a promotes vascular smooth muscle cell proliferation and migration through the ROCK/MLCK signalling pathway. Journal of Cellular and Molecular Medicine, 23(5), 3696-3710.

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Serum Lipid Measurement Protocol

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Blood lipid levels were determined by using total cholesterol assay kit, triglyceride assay kit and low-density lipoprotein cholesterol assay kit (Nanjing Jiancheng Institute of Bioengineering, China). Briefly, samples, as well as blank distill water and calibrator were mixed with working solution in proportion, then incubated at 37°C for 10 min. Samples were analyzed at wavelength 510 nm, light path 0.5 cm using automatic biochemical analyzer (Chemray240, Rayto, China) to measure the concentration of serum triglyceride (TG), total cholesterol (TC), and low-density lipoprotein (LDL).

Song Z., Zhong X., Li M., Gao P., Ning Z., Sun Z, & Song X. (2021). 1-MNA Ameliorates High Fat Diet-Induced Heart Injury by Upregulating Nrf2 Expression and Inhibiting NF-κB in vivo and in vitro. Frontiers in Cardiovascular Medicine, 8, 721814.

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