Anti-human primary antibodies to ERα (sc8002), GCS (sc50551), acid ceramidase (sc28486), and secondary horseradish peroxidase-conjugated antibodies for mouse (sc2005), rabbit (sc2054), and goat (sc2020) IgG were from Santa Cruz Biotechnology (Santa Cruz, CA). Anti-cleaved caspase-3 (#9661), -cleaved PARP (#5625), -p21 (#2964), -GAPDH (#97166), and - cyclinD1 (#2926) were from Cell Signaling (Danvers, MA). Anti-ERα (108398), -SPHK1 (ab77107), -SPHK2 (ab37977), -Ki67 (ab92742) were from Abcam (Cambridge, MA). Anti-β-actin (A5441) was from Sigma (St. Louis, MO). The monoclonal antibody specific to C16- and C24-of ceramide (MID 15B4) and tamoxifen citrate were purchased from Enzo Life Sciences (Farmingdale, NY). Fingolimod (FTY720) HCl, and carboplatin were purchased from Selleck Chemicals (Houston, TX) and MedChem Express (Monmouth Junction, NJ).
Fingolimod fty720 hcl
Manufactured by Selleck Chemicals
Sourced in United States
Fingolimod (FTY720) HCl is a synthetic compound used as a research reagent. It functions as a sphingosine 1-phosphate receptor modulator.
Automatically generated - may contain errors
Lab products found in correlation
Cyclin d1, by Cell Signaling Technology (1 mentions)
Tamoxifen citrate, by Enzo Life Sciences (1 mentions)
Anti β actin a5441, by Merck Group (1 mentions)
Carboplatin, by Selleck Chemicals (1 mentions)
Anti iba1 antibody, by Fujifilm (1 mentions)
Anti inos antibody, by Santa Cruz Biotechnology (1 mentions)
Vpc23019, by Avanti Polar Lipids (1 mentions)
C2 ceramide, by Merck Group (1 mentions)
S6k1 inhibitor pf 4708671, by Bio-Techne (1 mentions)
3 protocols using fingolimod fty720 hcl
1
Antibody-Based Protein Detection Assay
2
Fingolimod Modulates Microglial Activation
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Fingolimod (FTY720) HCl was purchased from Selleckchem (Houston, TX, USA) and anti-Iba1 antibody was purchased from FUJI FILM Wako Pure Chemicals (Japan). Anti-iNOS antibody was purchased from Santa Cruz Biotechnology, while anti-Mannose Receptor (CD206) antibodies were purchased from Abcam. Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204), p44/42 MAPK (Erk1/2), Phospho-MEK1/2 (Ser217/221), MEK1/2, Phospho-JNK (Thr183/Tyr185), JNK, Phospho-p38MAPK (Thr180/Tyr182), p38 MAPK, and Na/K-ATPase antibodies were purchased from Cell Signaling Technology. The S1PR1 and S1PR3 antagonist VPC 23019 was purchased from Avanti Polar Lipids (Alabaster, USA).
3
Climbing Assay for Fly Lifespan
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Climbing assays were carried out according to previously described methods (Ng et al. 2009 (link)). In general, 30 flies per group were used for the assay and the experiment was replicated with three different sets of flies. To study the effects of drugs, flies were fed with cornmeal-agar medium supplemented with 250 μM C2 Ceramide (N-acetyl-d -sphingosine, Sigma-Aldrich), 250 μM Fingolimod (FTY720), HCl (Selleckchem) or 250 μM S6K1 Inhibitor (PF-4708671, Tocris) at day 25 post-eclosion till day 50.
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