Elan drc e system

The content of metal ions in AT‐MSCs was determined by inductively coupled plasma mass spectrometry (ICP‐MS). Samples were transferred by concentrated nitric acid into teflon vessels for microwave decomposition (Uniclever BMI−Z, Plazmatronics, Poland). A mixture of 3 mL nitric acid and 1 mL hydrofluoric acid (both Suprapur, Merck, Darmstadt, Germany) was used for sample decomposition. The decomposed samples were transferred to a 50 mL volumetric flask and after appropriate dilution spiked with an internal standard solution (¹⁰⁰Rh). Both the calibration solutions and the IS solution were prepared from solutions at a concentration of 1.000±0.002 g/L (Merck). Distilled and demineralized water (conductivity <0.1 μS/cm) (Millipore, Bedford, MA, USA) was used to prepare all solutions.
ICP‐MS measurements were performed using an Elan DRC‐e system (PerkinElmer, Concord, Canada) equipped with a concentric PTFE nebulizer, a cyclonic spray chamber, a high‐efficiency quartz torch, a dynamic reaction cell (DRC) for the elimination of spectral interferences and the Gilson 212 peristaltic pump. Monitored nuclides included ⁵⁷Fe, ⁶⁶Zn and ⁵⁵Mn.
Measurement conditions were: Plasma power 1 100 W, sample uptake 1.2 mL/min, nebulizer Air Flow 0.75 L/min, plasma Air Flow 11 L/min, auxiliary Air Flow 1 L/min, dwell time 50 ms per nuclide, sweeps/reading 10, total acquisition time 20 s.