Hepa1-6 (ATCC, Manassas, VA) hepatocytes were maintained in DMEM (Invitrogen/Life Technologies, Grand Island, NY) supplemented with 10% FBS and 1% penicillin/streptomycin. Murine CT26 colonic cells (ATCC) were maintained in RPMI media (Invitrogen) supplemented with 20% FBS and 1% penicillin/streptomycin. Human Caco2 colonic cells (ATCC) were maintained in EMEM (ATCC) supplemented with 20% FBS and 1% penicillin/streptomycin. Primary mouse small intestine epithelial cells were purchased from Cell Biologics, Inc (Chicago, IL) and cultured in epithelial cell medium containing 0.1% EGF, 0.1% insulin-transferrin sodium selenite, 0.1% hydrocortisone, 5% FBS, 1% L-Glutamine, and a 1% antibiotic-antimycotic solution on gelatin (Sigma-Aldrich, St. Louis, MO)-coated plates. GW4064 was purchased from Sigma-Aldrich.
Human caco2 colonic cells
Manufactured by American Type Culture Collection
Caco-2 cells are a human colonic adenocarcinoma cell line that spontaneously differentiate in culture to form a monolayer of polarized cells resembling the intestinal epithelium. These cells are widely used as an in vitro model for studying intestinal absorption, transport, and metabolism of drugs and other compounds.
Automatically generated - may contain errors
Lab products found in correlation
Gw4064, by Merck Group (2 mentions)
Rpmi media, by Thermo Fisher Scientific (2 mentions)
Hepa1 6, by American Type Culture Collection (2 mentions)
Murine ct26 colonic cells, by American Type Culture Collection (2 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (2 mentions)
Primary mouse small intestine epithelial cells, by Cell Biologics (2 mentions)
Antibiotic antimycotic solution, by Merck Group (1 mentions)
2 protocols using human caco2 colonic cells
1
Cultivation of Diverse Cell Lines
2
Cell Culture of Hepatic and Intestinal Cells
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Hepa1-6 (ATCC, Manassas, VA, USA) hepatocytes were maintained in DMEM (Invitrogen/Life Technologies, Grand Island, NY, USA) supplemented with 10% foetal bovine serum (FBS) and 1% penicillin/streptomycin. Murine CT26 colonic cells (ATCC) were maintained in RPMI media (Invitrogen) supplemented with 20% FBS and 1% penicillin/streptomycin. Human Caco2 colonic cells (ATCC) were maintained in EMEM (ATCC) supplemented with 20% FBS and 1% penicillin/streptomycin. Primary mouse small intestine epithelial cells were purchased from Cell Biologics Inc. (Chicago, IL, USA) and cultured in epithelial cell medium containing 0.1% epidermal growth factor, 0.1% insulin-transferrin sodium selenite, 0.1% hydrocortisone, 5% FBS, 1% L-glutamine and a 1% antibiotic-antimycotic solution on gelatin (Sigma-Aldrich, St Louis, MO, USA)-coated plates. GW4064 was purchased from Sigma-Aldrich.
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