VP64 and VP160 sequences containing 4 or 10 copies of the nucleotides encoding VP16 minimal transactivator sequence DALDDFDLDML18 (link) (Table S3 ) were synthesized (GenScript) and subcloned in the N-terminus of dCas9 in the pCI-neo vector (Promega). The dCas9. VP64-p65-Rta (VPR) construct19 (link) which served as the template for the various PCR amplifications was purchased from Addgene (#63,798; Cambridge, MA, USA). All VP-based constructs were subcloned into the pCI/neo expression vector (Promega) for uniformity. The Cas-effector with synthetic activation motif (SAM) targeting the mouse TIMP1 gene was purchased from Santa Cruz Biotechnology (sc-423402-ACT, Santa Cruz, CA, USA).
Dcas9 vp64 p65 rta construct19
Manufactured by Addgene
Sourced in United States
The DCas9. VP64-p65-Rta (VPR) construct is a gene editing tool that combines a deactivated Cas9 (dCas9) protein with a tripartite activation domain consisting of VP64, p65, and Rta. This construct is intended to provide robust transcriptional activation of target genes when directed to specific genomic loci by guide RNA.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using dcas9 vp64 p65 rta construct19
1
Synthetic Transcriptional Activators for CRISPR Modulation
2
Synthetic Transcriptional Activators for CRISPR Modulation
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VP64 and VP160 sequences containing 4 or 10 copies of the nucleotides encoding VP16 minimal transactivator sequence DALDDFDLDML18 (link) (Table S3 ) were synthesized (GenScript) and subcloned in the N-terminus of dCas9 in the pCI-neo vector (Promega). The dCas9. VP64-p65-Rta (VPR) construct19 (link) which served as the template for the various PCR amplifications was purchased from Addgene (#63,798; Cambridge, MA, USA). All VP-based constructs were subcloned into the pCI/neo expression vector (Promega) for uniformity. The Cas-effector with synthetic activation motif (SAM) targeting the mouse TIMP1 gene was purchased from Santa Cruz Biotechnology (sc-423402-ACT, Santa Cruz, CA, USA).
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