Synthetic human aβ1 42

Aβ1-42 solution was prepared as previously described [26 (link)]. Briefly, synthetic human Aβ1-42 (Peptide Institute, Osaka, Japan) was dissolved to 1 mM in 100% 1,1,1,3,3,3-hexafluoro-2-propanol (HFIP). The HFIP was dried and resuspended to a concentration of 5 mM in DMSO. To form oligomers, amyloid peptide was diluted to a final concentration of 100 μM with Ham’s F-12, incubated at 4°C for 24 h, and then immediately added to cultures at a final concentration of 5 μM.

Soluble oligomeric amyloid β_1–42 (oAβ_1–42) was prepared as described previously [11] (link). Briefly, synthetic human Aβ_1–42 (Peptide Institute, Osaka, Japan) was dissolved in 100% 1,1,1,3,3,3-hexafluoro-2-propanol at a concentration of 1 mM. This solution was completely dried by the vacuum desiccator. The obtained film was resuspended in dimethyl sulfoxide to a concentration of 5 mM, and diluted with Dulbecco's Modified Eagle Medium/F12 (Invitrogen, Carlsbad, CA, USA) at a concentration of 100 µM. This solution was incubated at 4°C for 24 h to obtain oAβ_1–42. A final concentration of 5 µM oAβ_1–42 was used in all experiments.