Total RNA from human chondrocytes and rat chondrocytes was extracted using TRIzol®. The RNA was reverse transcribed to cDNA by reverse transcription using an mRNA reverse transcription kit (CW2569, Cwbio) and miRNA reverse transcription kit (CW2141, Cwbio). Primers were designed and synthesized by Beijing Tsingke Biotechnology, and the sequences are listed in Table 1 . Real-time Quantitative PCR (RT-qPCR) was performed using fluorescence quantitative PCR kit (PIKOREAL96, Thermo). The fluorescence intensity during the reaction was recorded in real time. The gene expression was calculated by 2−ΔΔCt method compared with β-actin, U6, or 5S.
Cw2141
Manufactured by CWBIO
Sourced in China
The CW2141 is a high-precision laboratory equipment designed for a range of scientific applications. It features advanced technology to ensure accurate and reliable measurements. The core function of the CW2141 is to provide precise data collection and analysis capabilities for researchers and scientists.
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Lab products found in correlation
2 protocols using cw2141
1
Quantitative Analysis of Gene Expression
2
Quantitative miRNA expression analysis
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Total RNA was extracted from cells and reverse-transcribed into complementary DNA (cDNA) using TRIzol reagent (15596026, Thermo Fisher Scientific, MA, USA) and a reverse transcription kit (CW2141, CWBIO, Beijing, China). PCR was performed using UltraSYBR mixture (CW2601, CWBIO). U6 was used as the internal control for miRNA expression. Relative miRNA expression was determined by the 2−ΔΔCt method. The primer sequences are shown in Supplementary Table 1 .
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