Qiasymphony automated extraction system

Manufactured by Qiagen

Sourced in Germany

The QIAsymphony automated extraction system is a laboratory equipment designed for automated nucleic acid purification. It performs sample preparation and extraction of DNA, RNA, and other biomolecules from a variety of sample types. The system is capable of processing multiple samples simultaneously and provides consistent, high-quality results.

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Lab products found in correlation

Tissuelyser, by Qiagen (1 mentions) Rnase inhibitor, by Promega (1 mentions) Dsp virus pathogen mini kit, by Qiagen (1 mentions) Ez1 dna tissue kit, by Qiagen (1 mentions) Nexus copy number software v7, by BioDiscovery (1 mentions)

Spelling variants of this product

QIAsymphony (189 citations) QIAsymphony system (15 citations) QIAsymphony automated system (4 citations)

2 protocols using qiasymphony automated extraction system

Comprehensive Tissue Sampling and Analysis

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Samples (25 mg of tissue in 1 mL Phosphate buffered saline solution) from lungs, liver, kidneys, spleen, mediastinal lymphnodes, placenta, foetus and intestine content of the two animals were homogenated by Tissue Lyser (Qiagen GmbH—Hilden, Germany). Each organ was analyzed in triplicate, by sampling three different parts of the whole tissue, each one in turn analyzed in triplicate. In particular for the lungs we analyzed three samples from the left cranial lobe. Nucleic acid extraction was carried out from 200 µL of each organ homogenate by using QIAsymphony automated extraction system (Qiagen GmbH, Hilden, Germany) with the DSP Virus/Pathogen Mini kit (Qiagen GmbH, Hilden, Germany) according to the manufacturer’s instructions. Nucleic acids were eluted in 60 µL of elution buffer containing 40 unit/µL RNase inhibitor (Promega Corporation, Madison, Wisconsin, USA) and immediately analyzed by Real-time PCR.

Amoroso M.G., Lucifora G., Degli Uberti B., Serra F., De Luca G., Borriello G., De Domenico A., Brandi S., Cuomo M.C., Bove F., Riccardi M.G., Galiero G, & Fusco G. (2020). Fatal Interstitial Pneumonia Associated with Bovine Coronavirus in Cows from Southern Italy. Viruses, 12(11), 1331.

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Fetal and Parental DNA Profiling

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Following informed consent from the parents, DNA was extracted from fetal tissue using the EZ1 DNA Tissue Kit (Qiagen). DNA from the parents was isolated from peripheral blood leucocytes using a magnetic bead method and the QIAsymphony automated extraction system (Qiagen). Samples from I-1, I-2 and II-2 were run on CytoSNP-12v2 arrays (Illumina) and analysed in Nexus Copy Number software v7.5 (BioDiscovery, Hawthorne, CA, USA).

Pagnamenta A.T., Howard M.F., Wisniewski E., Popitsch N., Knight S.J., Keays D.A., Quaghebeur G., Cox H., Cox P., Balla T., Taylor J.C, & Kini U. (2015). Germline recessive mutations in PI4KA are associated with perisylvian polymicrogyria, cerebellar hypoplasia and arthrogryposis. Human Molecular Genetics, 24(13), 3732-3741.

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