Rabbit anti crth2

Paraffin-embedded sections of human colon from CD patients and controls were cut (5 μm) and deparaffinized. For immunohistochemistry, sections were microwaved for 2 × 5-min cycles in 10 mM citrate buffer, and processed by ABC method according to the manufacturer’s protocol (Vectastain ABC kit; Vector Laboratories, Burlingname, CA, USA). Sections were incubated with rabbit anti-CRTH2 (1:200; Acris Antibodies, Herford, Germany) ^{11 (link)}, visualized with 3-3′-diaminobenzidine (DAB) and counterstained with hematoxylin. CD4⁺ T cells were stained with a monoclonal mouse anti-human CD4 (clone 4B12; dilution 1:20; Labvision, Fremont, USA) as recommended by the suppliers. Images were taken with a high resolution digital camera (Olympus DP 50) and analyzed by Cell^A imaging software (Olympus, Vienna, Austria). Only contrast and brightness of images were adjusted. Sirius Red (Direct Red 80®, Sigma) was used to stain eosinophils in deparaffinized sections.

Paraffin-embedded sections of human colon from UC patients and controls were cut (10 μm) and deparaffinized, microwaved for 2 × 5 min cycles in 10 mM citrate buffer and then processed by ABC method (Vectastain ABC kit; Vector Laboratories, Burlingname, CA, USA) according to the manufacturer’s protocol. Sections were incubated with rabbit anti-CRTH2 (1:200; Acris, Herford, Germany) (34 (link)) or rabbit anti-DP1 (1:100; Cayman Chemicals, Ann Arbor, MI, USA) (9 (link)) visualized with 3-3′-diaminobenzidine and counterstained with haematoxylin. The specificity of the Abs was tested by omitting the primary Ab and by incubating with the respective blocking peptide provided by the manufacturers (see supplemental material S3). Images were taken with a high-resolution digital camera (Olympus DP 50), processed and analyzed by Cell^A imaging software (Olympus, Vienna, Austria). Only contrast and brightness of images were adjusted.