Bromophenol blue

A human high metastatic large cell lung cancer cell line (L9981) and low metastatic large cell lung cancer cell line (NL9980) were established from a human lung large cell carcinoma cell line (WCQH‐9801) in our laboratory.48 Cell lines were cultured in RMPI 1640 medium containing 10% fetal bovine serum (Gibco, Grand Island, NY, USA) at 37°C with 5% CO₂ incubator. Cy2, Cy3, and Cy5 were purchased from GE Healthcare (Piscataway, NJ, USA). Immobiline pH‐gradient (IPG) DryStrips (pH 3–10,24 cm), IPG buffer (pH 3–10), DryStrip cover fluid, thiourea, urea, dithiothreitol (DTT) , Pharmalyte (pH 3‐10), bromophenol blue, Commassie Brilliant Blue G‐250, Tris base, sodium dodecyl sulfate (SDS), and molecular weight marker were purchased from Amersham Biosciences (Uppsala, Sweden).
Modified trypsin (sequencing grade) was obtained from Promega (Madison, IL, USA). Sucrose and mannitol were purchased from Sango (Shanghai, China). All analytical grade chemicals and biochemicals were prepared with Milli‐Q deionized water (Millipore, Bedford, MA, USA).
Primary anti‐fumarate hydratase (sc‐27995), PRDX3 (sc‐23969), HSP75 (TR1) (sc‐13557), ERAB (sc‐58525), PCNA (sc‐56), and cytochrome c (SC‐13560) antibodies were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA), and anti‐PHB (#2426) and COX IV (#4844) antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA).