Benztropine

Manufactured by Merck Group

Sourced in France, United States, Sao Tome and Principe

Benztropine is a pharmaceutical product manufactured by Merck Group. It is a synthetic compound with the primary function of acting as an anticholinergic agent. The core function of Benztropine is to block the action of the neurotransmitter acetylcholine in the central and peripheral nervous systems.

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Lab products found in correlation

Miconazole, by Merck Group (2 mentions) Glutathione agarose, by Merck Group (2 mentions) Ecgonine, by Merck Group (2 mentions) Benzocaine, by Cayman Chemical (2 mentions) Benzoylecgonine, by Cayman Chemical (2 mentions) Benztropine, by Cayman Chemical (2 mentions) Procaine, by Cayman Chemical (2 mentions) Tropine, by Cayman Chemical (2 mentions) Neurobasal a medium, by Thermo Fisher Scientific (2 mentions) Lidocaine, by Merck Group (2 mentions) Scopolamine, by Merck Group (2 mentions) 3h cocaine, by PerkinElmer (2 mentions) B 27 supplement minus antioxidants, by Thermo Fisher Scientific (2 mentions) Anisodamine, by Cayman Chemical (2 mentions) 3 p fbt hydrochloride, by Cayman Chemical (2 mentions) Cocaine hydrochloride, by Merck Group (2 mentions) Protein a g plus agarose, by Santa Cruz Biotechnology (2 mentions) B27 supplement, by Thermo Fisher Scientific (2 mentions) Benzoic acid, by Merck Group (2 mentions) B27 without vitamin a, by Thermo Fisher Scientific (1 mentions)

6 protocols using benztropine

Time-Controlled Drug Delivery in EAE Model

Cited 2 times

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Alzet miniosmotic pumps (Durect, Cupertino, CA) were used for time-controlled drug delivery. Fourteen-day pumps (rate of infusion 0.25 μL/h, 100 μL total volume) were filled with either vehicle or 500 μM tuftsin (Sigma) (23 (link)–26 (link)), 18 μg/μl benztropine (Sigma), or both tuftsin and benztropine at the same doses and incubated overnight at 37°C before use. The pumps were implanted subcutaneously in the back of the mice under anesthesia 14-days post-MOG immunization. For experiments where tuftsin was administered prior to addition of benztropine, 7-days pumps containing vehicle or tuftsin alone were implanted on Day 14 post-MOG immunization and were replaced with 7-days pumps containing either vehicle or both tuftsin and benztropine at Day 21.

Thompson K.K., Nissen J.C., Pretory A, & Tsirka S.E. (2018). Tuftsin Combines With Remyelinating Therapy and Improves Outcomes in Models of CNS Demyelinating Disease. Frontiers in Immunology, 9, 2784.

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Metabolomics of Oligodendrocyte Differentiation

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All the endogenous metabolites used in this study were from Sigma-Aldrich. Benztropine, miconazole and triiodothyronine (T3) were purchased from Sigma-Aldrich (Saint Louis, MO). All the chemicals used in this study have a purity ≥ 95%.
Rat primary optic nerve OPCs were isolated by panning (>99% A2B5⁺) and cultured in poly-d-lysine (10 µg/ml)-coated TC dishes in OPC culture media (Neurobasal Media, Invitrogen) supplemented with B27 without vitamin A (Invitrogen), 1× nonessential amino acids, 1× Glutamax, 1× anti-anti, β-mercaptoethanol and PDGFαα (50 ng/ml; Peprotech) at 37 °C with 5% CO₂. The culture medium was replaced every 48 h, and cells were collected before the confluency reached 60% to maintain a naive state. For differentiation, OPCs were plated at 1 million cells in one 10-cm Petri dish filled with differentiation media, which is identical to the culture media but with PDGF-AA at 2 ng/ml. T3 and DMSO were used as the positive control and negative control, respectively. Five replicates of cells were collected at different times (0, 3 and 6 d) for metabolomics studies.

Beyer B.A., Fang M., Sadrian B., Montenegro-Burke J.R., Plaisted W.C., Kok B.P., Saez E., Kondo T., Siuzdak G, & Lairson L.L. (2017). Metabolomics-based discovery of a metabolite that enhances oligodendrocyte maturation. Nature chemical biology, 14(1), 22-28.

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Benztropine and Oxaliplatin Effects on Cell Viability

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All cells (2 × 10⁴ per well) were seeded in 96-well plates (Sigma-Aldrich, Saint-Quentin Fallavier France) and incubated for 24 h with 7.5 to 30 μM of benztropine (Sigma-Aldrich, Saint-Quentin Fallavier France) and treated with 0 to 100 μM of oxaliplatin (Accord Healthcare Limited, Lille, France). Cell viability was assessed by a crystal violet assay, and results are expressed as the mean percentage of viable cells ± SEM versus cells not exposed to oxaliplatin (100% viability). Cellular production of ROS and reduced glutathione (GSH) were assessed by spectrofluorimetry with 2′,7′-dichlorodihydrofluorescein diacetate (H2DCFDA, #6883 Sigma-Aldrich, Saint-Quentin Fallavier France) and monochlorobimane (#69899 Sigma-Aldrich), respectively [49 (link)].

Cerles O., Gonçalves T.C., Chouzenoux S., Benoit E., Schmitt A., Bennett Saidu N.E., Kavian N., Chéreau C., Gobeaux C., Weill B., Coriat R., Nicco C, & Batteux F. (2019). Preventive action of benztropine on platinum-induced peripheral neuropathies and tumor growth. Acta Neuropathologica Communications, 7, 9.

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Dopamine Transporter Binding Assay

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Neurobasal-A medium (no glucose, no sodium pyruvate), B-27 supplement, and B-27 supplement minus antioxidants were purchased from Life Technologies. Cocaine hydrochloride, atropine, benzoic acid, benz tropine, ecgonine, lidocaine, scopolamine, and glutathione agarose were purchased from Sigma-Aldrich. Anisodamine, benzocaine, benzoylecgonine, benz tropine, 3-p-FBT (hydrochloride), procaine, and tropine were purchased from Cayman Chemical. Protein A/G PLUS-agarose was purchased from Santa Cruz Biotechnology. [³H]cocaine was purchased from PerkinElmer Life Sciences. FLAG-DAT plasmid (pcihygroflagsynhDAT) was a gift from Jonathan Javitch, Columbia University, New York, NY (Addgene plasmid #19990) (77 (link)). PDGF-TMD-GFP plasmid (pFU-no toxin-PE) was a gift from Ines Ibañez-Tallon, Rockefeller University, New York, NY (Addgene plasmid #24149) (78 (link)).

Harraz M.M., Malla A.P., Semenza E.R., Shishikura M., Singh M., Hwang Y., Kang I.G., Song Y.J., Snowman A.M., Cortés P., Karuppagounder S.S., Dawson T.M., Dawson V.L, & Snyder S.H. (2022). A high-affinity cocaine binding site associated with the brain acid soluble protein 1. Proceedings of the National Academy of Sciences of the United States of America, 119(16), e2200545119.

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Monoclonal AQP4-IgG and Compound Screening

Cited 1 time

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Purified human monoclonal recombinant AQP4-IgG rAb-53 (AQP4-IgG) was provided by Dr. Jeffrey Bennett (Univ. Colorado Denver) as described [45 (link)]. Control human IgG (control-IgG) was purchased from Pierce Biotechnology (Rockford, IL, USA). Human complement (HC) was purchased from Innovative Research (Novil, MI, USA). Test drugs included clobetasol, miconazole, benztropine, clemastine, fumarate, retinoic acid and citicolone (Sigma-Aldrich, St. Louis, MO, USA), enprofylline, olesoxime and quetiapine fumarate (Santa Cruz Biotechnology, Dallas, TX, USA), GC-1 and quercetin (Tocris Bioscience, Bristol, UK), fasudil (Tszchem, Lexington, MA, USA), and Y-27632 (BD Biosciences, San Jose, CA, USA); Triiodothyronine (T3, Calbiochem, Billerica, MA, USA) was used as positive control. Drugs were dissolved in 2.5 % DMSO + 2 % solutole in PBS. Unless otherwise specified all other chemicals and media were purchased from Sigma-Aldrich.

Yao X., Su T, & Verkman A.S. (2016). Clobetasol promotes remyelination in a mouse model of neuromyelitis optica. Acta Neuropathologica Communications, 4, 42.

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Neuronal Cell Culture Reagents

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Neurobasal-A medium (no glucose, no sodium pyruvate), B-27 supplement and B-27 supplement minus antioxidants were purchased from Life Technologies (Grand Island, NY). Cocaine hydrochloride, atropine, benzoic acid, benz tropine, ecgonine, lidocaine, scopolamine and glutathione agarose were purchased from Sigma-Aldrich (St. Louis, MO). Anisodamine, benzocaine, benzoylecgonine, benz tropine, 3-p-FBT (hydrochloride), procaine, and tropine were purchased from Cayman Chemical (Ann Arbor, Michigan). Protein A/G plus-agarose was purchased from Santa Cruz Biotechnology (Paso Robles, CA). [ 3 H]cocaine was purchased from PerkinElmer Life Sciences (Boston, MA). FLAG-DAT plasmid (pcihygroflagsynhDAT) was a gift from Jonathan Javitch, Columbia University (Addgene plasmid # 19990) 28 . PDGF-TMD-GFP plasmid (pFU-no toxin-PE) was a gift from Ines Ibanez-Tallon (Addgene plasmid # 24149) 29 .

Harraz M.M., Malla A.P., Semenza E.R., Shishikura M., Hwang Y., Kang I.G., Song Y.J., Snowman A.M., Cortés P., & Snyder S.H. (2020). Cocaine receptor identified as BASP1.

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