Unless otherwise stated, all reactions were performed under an inert atmosphere of nitrogen. UV-vis spectra were recorded on an Agilent 8453 UV-vis spectrometer equipped with an Agilent 89090A thermostat (±0.1 °C) at 25 °C. IR spectra were recorded on a Spectrum Spotlight 200 FT-IR microscope. Mass spectra were recorded on a Bruker APEX IV FT-ICR mass spectrometer (ESI) or AB Sciex MALDI-TOF mass spectrometer. Simulated mass spectra were obtained from the website http://www.chemcalc.org . NMR spectra were recorded on a Bruker ARX400 400 MHz or AVANCE III 500 MHz NMR spectrophotometer. The HeLa cells were obtained from Peking University Health Science Center. For the optical measurements in liquid solution, spectroscopic-grade dimethyl sulfoxide was used as purchased from Alfa-Aesar. Anhydrous 1,2,4-trichlorobenzene (TCB), diisobutyl aluminium hydride (DIBAL-H), sarcosine, and polyformaldehyde were purchased from J&K Scientific and used as received. Anhydrous CH2Cl2 was distilled from calcium hydride and H2O was obtained from Milli-Q Integral.
Maldi tof mass spectrometer
Manufactured by AB Sciex
Sourced in United States
The MALDI-TOF (Matrix-Assisted Laser Desorption/Ionization Time-of-Flight) mass spectrometer is an analytical instrument used for the identification and characterization of molecules. It utilizes a laser to ionize and vaporize samples, and a time-of-flight analyzer to measure the mass-to-charge ratio of the ionized particles.
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3 protocols using maldi tof mass spectrometer
1
Spectroscopic Characterization of Organic Compounds
2
MALDI-TOF Mass Spectrometry of Bacterial Antagonists
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The methanol extracts of the culture filtrates of the four antagonistic bacteria were analyzed using the 5800 matrix-assisted laser desorption ionization–time-of-flight (MALDI-TOF) mass spectrometer (AB SCIEX, Redwood, CA, USA) operated in the positive reflectron mode; α-cyano-4-hydroxycinnamic acid was used as the matrix (prepared by dissolving 5 mg in 1 ml of 50:50 acetonitrile: water containing 0.1% trifluoroacetic acid) (Velho et al., 2011 (link)).
3
Agar Hydrolysis via Thin-Layer Chromatography
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Thin-layer chromatography (TLC) was performed to identify hydrolysis products of AgaDL6 towards agar [25] . After incubating purified AgaDL6 with 1% agar at different times at optimum conditions, the hydrolysis reaction was stopped by heating in a boiling water bath for 10 min. The reaction mixture was spotted on silica gel 60 TLC plates (Merck, Germany). The plates were developed with n-butanol-acetic acid-water (2:1:1 by volume) solution. Spots were visualized by spraying with 20% H 2 SO 4 in methanol and heating at 120°C for 2 min. Neoagarobiose (NA2), neoagarotetraose (NA4), neoagarohexaose (NA6), neoagarooctaose (NA8) and neoagarodecaose (NA10) were used as standards. The molecular mass of the enzymatic products was determined using a matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometer (AB Sciex, USA). The matrix for all samples was 2,5-dihydroxybenzoic acid (DHB) (10 mg/ml DHB in 30/70 acetonitrile/water with 0.1% TFA final concentration) and the ratio of the sample and matrix was 1:1. The measured data were analyzed and exported using Data Explorer Software 4.11 (AB Sciex).
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