rvWF samples were run in reducing conditions using the XCell SureLock Mini electrophoresis system (Invitrogen, CA, USA) on a Novex Tris-Glycine 3-8% precast gel (Invitrogen). After blotting, the membrane was incubated with a polyclonal rabbit anti-vWF (DAKO, Glostrup, Denmark), and then with a polyclonal anti-rabbit-HRP antibody (for further details see the Online Supplementary Methods).
Polyclonal rabbit anti vwf
Manufactured by Agilent Technologies
Sourced in Denmark, Netherlands, Belgium
Polyclonal rabbit anti-vWF is a laboratory product used for the detection and analysis of von Willebrand factor (vWF) in biological samples. It is produced in rabbits and recognizes multiple epitopes on the vWF protein.
Automatically generated - may contain errors
Lab products found in correlation
Polyclonal rabbit anti mouse, by Agilent Technologies (1 mentions)
Rabbit polyclonal anti cd3, by Agilent Technologies (1 mentions)
Pannoramic scan 2 digital slide scanner, by 3DHISTECH (1 mentions)
Monoclonal anti αsma, by Agilent Technologies (1 mentions)
Rabbit polyclonal anti caspase 3, by Cell Signaling Technology (1 mentions)
4 protocols using polyclonal rabbit anti vwf
1
Western Blot Analysis of vWF
2
Antibodies and Conjugates for PUUV Detection
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
We made use of the following antibodies and conjugates: polyclonal rabbit anti- VWF, HRP labeled polyclonal goat anti-rabbit IgG and polyclonal rabbit anti-mouse (All from Dako, the Netherlands). FITC labeled monoclonal anti-CD31 (Sigma Aldrich, USA), polyclonal rabbit anti-CD41 (Perbio Science, the Netherlands), polyclonal rabbit anti-CD3 (Dako), polyclonal rabbit anti-PUUV nucleoprotein (BEI Resources, USA), monoclonal anti-PUUV glycoprotein (HY Test, Finland), monoclonal anti-ανβ3 integrin (Abcam, UK), monoclonal anti-vitronectin (Novus Bio, USA), polyclonal rabbit-anti PAI-1 (Bio Connect, the Netherlands), polyclonal rabbit anti-tissue factor (TF; Bio Connect), human serum from a recovered PUUV case described in Goeijenbier et al. (2011) (link) retrieved after informed consent and ethical board approval. Antibodies and conjugate were diluted in dilution buffer, which consisted of PBS with 0.5% bovine serum albumin, 2% NaCl and 1% normal goat serum.
3
Compatibility of microCT and IHC Staining
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
To test the compatibility of microCT imaging with subsequent immunohistochemical staining, we labelled paraffin sections of one microCT scanned specimen with the following antibodies and employing established protocols: monoclonal mouse‐anti‐EGFR [Clone 4575] (1:200 in PBS) (NeoBiotechnlogies; cat. nr. 1956‐MSM25‐P1), monoclonal rabbit‐anti‐EGF [3H11L6] (1:100 in PBS) (Thermo Fisher Scientific; cat. nr. 701538), monoclonal mouse‐anti‐Desmin [Clone D33] (1:300 in PBS) (Dako, Agilent; cat. nr. M0760), monoclonal mouse‐anti‐Vimentin [Clone V9] (1:500 in PBS) (Dako, cat. nr. M0725), monoclonal mouse‐anti‐SMA [Clone 1A4] (1:1000 in PBS) (Dako, cat. nr. M0851) and polyclonal rabbit‐anti‐vWF (1:7000 in PBS) (Dako, cat. nr. A0082). DAB‐stained sections were counterstained with haematoxylin and mounted using DPX (Fluka, Buchs, CH). Slides were imaged with a Pannoramic scan II digital slide scanner (3DHISTECH, Budapest, HU) with 20x objective magnification and a pixel size 0.5 μm.
4
Quantification of Renal Microvascular Markers
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All immunostainings of following primary antibodies: monoclonal anti-αSMA, polyclonal goat anti- vimentin and polyclonal rabbit anti-vWF (DakoCytomation, Heverlee, Belgium), monoclonal mouse anti-neutral endopeptidase, monoclonal anti-fibronectin (Lab Vision, Fremont, CA, USA), polyclonal rabbit anti-caspase 3 and rabbit monoclonal anti-PDGF receptor β (Cell Signaling Technology, Leiden, The Netherlands) were performed as extensively detailed previously. [37 (link)–39 (link), 41 (link)]
Two independent investigators (L.G. and A.P.) blind to the group origin of the rats performed all quantifications. Quantification of vWF+ peritubular capillaries were evaluated at x40 magnification in 30 fields (2.52 mm² of kidney tissue) and expressed as the number of vWF+ peritubular capillaries per field. Quantification of αSMA and vimentin immunostainings was performed using computer-assisted morphometric analysis (ImageJ-National Institute of Health, city, USA).
Two independent investigators (L.G. and A.P.) blind to the group origin of the rats performed all quantifications. Quantification of vWF+ peritubular capillaries were evaluated at x40 magnification in 30 fields (2.52 mm² of kidney tissue) and expressed as the number of vWF+ peritubular capillaries per field. Quantification of αSMA and vimentin immunostainings was performed using computer-assisted morphometric analysis (ImageJ-National Institute of Health, city, USA).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!