Thermo scientific spectrometer helios episilon

The protein concentrations of the samples were determined using the Bradford Assay—Sigma [22 ]. Bovine serum albumin (BSA- Fisher Scientific) standards of known protein concentration varying from 0 to 2 mg/ml were added to Bradford reagent in disposable cuvettes (Fisher Scientific) and the absorbance was measured spectrophotometrically at 595nm in a Thermo Scientific Spectrometer Helios Episilon (Fisher Scientific). The absorbance of the known protein standards were plotted using a linear regression. The mitochondrial fractions were diluted 1 μl in 100 μl Tris Buffer and added to Bradford reagent before the absorbance was measured. The absorbance was plotted against the BSA standard curve to determine the estimated protein concentration (mg/ml).

The protein concentrations of the samples were determined using the Bradford Assay - Sigma [64 ]. Bovine serum albumin (BSA- Fisher Scientific) standards of known protein concentration varying from 0 to 2mg/ml were added to Bradford reagent in disposable cuvettes (Fisher Scientific) and the absorbance was measured spectrophotometrically at 595 nm in a Thermo Scientific Spectrometer Helios Episilon (Fisher Scientific). The absorbance of the known protein standards was plotted using linear regression. The mitochondrial fractions were diluted 1 μl in 100 μl Tris Buffer and added to Bradford reagent before the absorbance was measured. The absorbance was plotted against the BSA standard curve to determine the estimated protein concentration (mg/ml).