Probetec et chlamydia trachomatis and neisseria gonorrhoeae amplified dna assay

Acquiring an incident STI was defined as a positive laboratory test result for a new Chlamydia, Gonorrhea, or Trichomonas infection at the baseline, 6-month, or 12-month assessments. Participants provided 2 vaginal swabs at each of the 3 assessments. One swab was evaluated for Neisseria gonorrhoeae (GC) and Chlamydia trachomatis (CT) using the Becton Dickinson ProbeTec ET Chlamydia trachomatis and Neisseria gonorrhoeae Amplified DNA Assay (Sparks, MD). A second vaginal swab was tested for Trichomonas vaginalis (TV) using Taq-Man PCR. The Caliendo Laboratory developed and validated this test which employs a homogenous kinetic PCR to amplify and detect a conserved part of a repeated DNA fragment of TV. All assays were conducted at the Emory University, Department of Pathology, Caliendo Research Laboratory. Women testing positive were provided directly observable single-dose treatment, received risk-reduction counseling per CDC recommendations, and were encouraged to refer sex partners for treatment. The county health department was notified of reportable STIs.

Alcohol use was assessed by asking participants the frequency with which they consumed alcohol during the past 30 days at baseline. Alcohol use was categorized as women who consumed alcohol versus women who abstained from alcohol.
Incident STI infection was defined as a positive laboratory test result for a new chlamydia, gonorrhea, or trichomonas infection. Participants provided two vaginal swabs at each of the three assessments. One swab was evaluated for Neisseria gonorrhoeae (GC) and Chlamydia trachomatis (CT) using the Becton–Dickinson ProbeTec ET Chlamydia trachomatis and Neisseria gonorrhoeae Amplified DNA Assay (Sparks, MD). A second vaginal swab was tested for Trichomonas vaginalis (TV) using Taq-Man polymerase chain reaction (PCR). The Caliendo Laboratory developed and validated this test which employs a homogenous kinetic PCR to amplify and detect a conserved part of a repeated DNA fragment of TV. All assays were conducted at the Emory University, Department of Pathology, Caliendo Research Laboratory. Women testing positive were provided directly observable singledose treatment, received risk-reduction counseling per CDC recommendations, and were encouraged to refer sex partners for treatment. The county health department was notified of reportable STIs.